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Questions related to RNA Extraction
The micro organism is anaplasma ovis, and it can't be cultured. I have purified anaplasma ovis stored in liquid nitrogen using glycerol stock. Kindly share the protocol and guide which kit give...
07 November 2017 3,676 2 View
Does anyone here can provide me some background for generating the transgenic line in zebrafish? I wish to generating GFP specific expression in microglia by inserting EGFP marker in...
11 October 2017 8,815 1 View
Hi everyone, I would like to do correlation analysis qPCR (2^(-delta delta Cq)) vs RNA-seq data in terms of fold change. In my qPCR, I have two conditions and one housekeeping genes to be...
19 September 2017 1,961 4 View
I have frozen samples lysed in RLT buffer and I would like to use the Zymo kit to isolate RNA. May this affect the yield/purity of the extracted RNA? In principle, the composition of the RLT...
14 September 2017 2,853 2 View
Can anyone help me to identify these Antarctic serpulids at least until genus? I know the photos are not good but if anyone has any suggestion to have an idea, it will be great. Many thanks! Blanca
12 September 2017 9,182 0 View
I could not find any published ones, so I hope to find some unpublished ones somewhere.
24 August 2017 2,800 0 View
Can anyone kindly tell me what amount of rice seedling leaves and root is required for isolation of good quantity of RNA ? Should I weigh the freshly chopped samples before homogenizing with lN2...
25 July 2017 432 8 View
hi i want to extract RNA from white blood cells for Real Time PCR and i need first increase white cells. i isolated buffy coat and cultured it with PHA for 72 h and then extracted RNA. but RNA...
23 July 2017 2,704 2 View
I was extracting RNA from honey bee midguts for qPCR using Trizol and my A260/280 values prior to cDNA generation are all around 2.5; my collaborator suspects Trizol contamination. Do you agree...
20 July 2017 601 3 View
I try already 2 protocols but there are not good enough, the nanodropo and the electrophoresis gel does not give us good results!
18 July 2017 8,686 3 View
I plan on isolating total RNA from exosomes of serum and plasma and would want to use a column based kit, since Qiagen miRNEasy kit is not fetching great results. Can I use RNeasy mini kit to...
18 July 2017 4,102 3 View
Hi, i would like to know if anyone tried the Direct-zol RNA extraction kit by Zymo Research. They claim it to be superfast, have a good yield and be able to purify also small RNAs. I'd use it on...
17 July 2017 10,043 2 View
Sampling methods for plant RNA study were different from the previous studies related the gene expression. Some researcher was collected the youngest leaf. Another researcher was collected fully...
13 July 2017 4,965 5 View
We used FNA paraffin-embedded hepatic tissues from our study population to evaluate some gene expression. But already we got week results in RNA extraction and also in RT-PCR. We extracted RNA by...
12 July 2017 2,120 3 View
We had the following A human blood sample had RNA concentration of 142,85 ng/ul, we used 3,43 ul in 20 ul (with a mass of 490 ng) using the iscript kit. Nanodrop reading for this blood sample...
12 July 2017 9,325 3 View
Has somebody know a protocol to simultaneusly perform Edu and in situ fluorescent hybridization to detect mRNA expression?
09 July 2017 7,370 0 View
I am doing an rna extraction from tomato leafs, using the Trizol protocol. But when I add chloroform, and centrifuged the samples, the hidrofilic portion gets a pink coloring. And the color keeps...
04 July 2017 6,808 6 View
Hi, I am working with cultured cells and I need to send some samples abroad for RNA extraction. I do not have Trizol reagent or RNAlater as yet, I ordered but I won't get them in time to ship my...
03 July 2017 5,292 4 View
I'm currently working with an organism which has no established endogenous control mirna that I could use to normalize qpcr data. I'm thinking about instead using an exogeous control mirna such as...
28 June 2017 1,758 3 View
My aim is to measure microRNA using Luminex from archived samples, so basically formalin fixed paraffin embedded, and I don't know if I can extract good quality (and stable) RNA from this kind of...
26 June 2017 4,299 2 View
I have done my qRT-PCR and the results showed that down regulation of my target gene compared to the untreated control. But the protein expression showed up regulation (>1) if compared to the...
26 June 2017 922 0 View
I'm having problems with qpcr. This primer have been tested in another lab and it worked fine. It has late amplification (like 30 cycles) but in my samples I get amplification in like 45 cycles....
21 June 2017 2,420 2 View
The PBMCs were suspended in RNALter and then stored at -80oc. Now I want to retrieve the PBMCs and then extract the RNA (inc the microRNAs) - does anyone have advice about centrifuge speeds/...
20 June 2017 6,227 3 View
Hi all, So I want to freeze zebrafish embryos to later (1 week) extract RNA with trizol + RNeasy kit an perform RNA-seq. Would you freeze the embryos in TRIzol or in RNAlater? Does to freezing...
19 June 2017 4,330 4 View