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Questions related to RNA Extraction
Taking 5 ml blood i should get a good yield of DNA as the birds have nucleated rbc can anyone please tell me a proper phenol choloroform method to solve it
18 May 2017 10,044 2 View
I have extracted RNA from avian blood using TRIzol from ambion. After a 3M sodium acetate / ethanol precipitation, the RIN number reads >8 on the bioanalyzer. I want to treat the RNA with TURBO...
18 May 2017 3,245 5 View
Hi AllI am using Trizol LS reagent for RNA and DNA extraction from Grape-vine leaf tissue, but I couldn't find any RNA and DNA. I found big pellet and a bit it is hard to dissolve. I check the...
16 May 2017 5,492 3 View
I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...
04 May 2017 6,174 4 View
The upper aqueous phase appears in the bottom of the tube.
02 May 2017 8,854 8 View
Has any one tried RNA extraction from ,72 hours old bone marrow sample EDTA blood
01 May 2017 6,032 5 View
Hi, I was wondering, if it is possible to extract total RNA and DNA from insect which have been fixed or stored in 70% ethanol at RT and some specimen at 4 degree. The isolated total RNA would be...
29 April 2017 4,810 3 View
I'm having problems with my RNA extraction with Trizol. Basically, I'm growing my plants in semi-sterile pouches (basically a plastic bag with a filter paper that allows the shoot to go up and...
26 April 2017 1,050 6 View
I extracted total RNA from frozen PBMCs using Norgen's total RNA extraction kit so that I could retain microRNAs for a small RNA sequencing experiment. The tapestation trace is bizarre! The...
26 April 2017 2,872 3 View
Hello All, I'm wondering what's the best plant total RNA extraction kit to use, if the down stream assay will be qPCR? Also this is for corn leaves and roots. I've been using the Spectrum (TM)...
25 April 2017 821 2 View
I want to preserve breast tissues for RNA extraction later ( after 2-4 months). is it possible preserve those tissues at -80 or at -20 without preservative reagents ( such as RNA later or...
23 April 2017 9,238 4 View
I am working on the next generation sequencing of EAV from horse semen samples. As my attempts of isolating virus on RK13 cell cultures were unsuccessful I have decided to use RNA material...
19 April 2017 2,289 3 View
I'm planning to extract the RNA from my bacterial cultures , but the reagents for the Reverse Transcriptase haven't arrived yet. How long could I keep the RNA frozen?
19 April 2017 2,768 6 View
Hello everyone. I have some problems with a RNA extraction. I used the trizol reagent to extract RNA from tissues of leaves and roots under drought stress. When I dissolve the RNA in water, RNA...
16 April 2017 9,576 3 View
I want to check expression of certain genes of bacteria. But bacterial preparation with different treatment is taking long time. This is why i want to store bacterial pellet after centrifugation...
14 April 2017 3,182 3 View
Dear all, is there anyone which experienced to homogenate tissues in trizol, but did not proceed immediately with RNA extraction? I mean, is it possible to homogenate in trizol and store your...
13 April 2017 6,290 3 View
Hi all, is there any protocol to purify high quality RNA from rodent samples? I checked quite few articles, but the RNA quality does not meet the highly pure criteria.
10 April 2017 1,739 2 View
I extracted RNA from my cells cultured in soft agar culture system by TRIZOL, but unfortunately pollution in RNA yield is high so I couldn't detect genes expression by real time PCR. Any one has...
08 April 2017 1,730 3 View
Hi everyone! Fungi total RNA was extracted by mirVana miRNA isolation kit using total RNA extraction protocol, but the RNA profile shows a large portion of small RNA, which seems unlike a...
07 April 2017 2,335 4 View
I have been isolating RNA from CD14 monocytes purified from PBMCs but the yield has been low. I've read that DNase treatment can result in lower RNA yields, but I want to ensure that these results...
06 April 2017 4,132 4 View
We are trying to detect gene amplification in canine tissue sections (histomorphology is important). The amplified region is about 60 kb in length. What is the best way to prepare the probe,...
04 April 2017 8,893 2 View
Dear researchers, I have attempted to do a gene expression study. The first step was isolate the RNA. I have checked the RNA yield using Nanodrop, which had the following nucleic Acid...
31 March 2017 8,021 3 View
I am trying to isolate a mRNA (1400 bases) from Dog placenta with trizol reagent. Every time we are getting degraded RNA. We have stored the 100mg sample in 300ul trizol reagent and stored at -80....
30 March 2017 1,303 2 View
I am trying to isolate DNA from the conidia of fungus using SDS. The conidia is directly isolated from plant (not cultured) and seems to have tough cell wall and somehow i managed to crush to...
30 March 2017 6,692 3 View