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Questions related to RNA Extraction
Has anyone had success with isolating RNA from FFPE sections that was sufficient in quantity and quality to make cDNA for RT-PCR? If so, what kit/protocol did you use for the RNA isolation, how...
16 June 2017 7,923 1 View
Hi all! :) I want to extract RNA from zebrafish embryos, 3dpf. My limitation will be the number of fish needed to gain enough material after (1) extracting total RNA (2) doing poly A...
15 June 2017 5,471 7 View
Hi, I would like to extract RNA from biofluids, and we have a home-made protocol that I got from a collaborator, that uses Zymo spin columns. However, we have bunches of miRNeasy micro minelute...
14 June 2017 8,512 1 View
If yes then how long can you keep such tissues in -80C without the getting degraded?
13 June 2017 992 1 View
Badly need some help as I did not find any exact protocol for my experiment anywhere, I am working with human platelet to find out the effect of charcoal on the expression of platelet glycoprotein...
12 June 2017 6,259 3 View
hello do Whatman® FTA® cards preserve RNA of a sample? for example, I am using Whatman® FTA® cards to collect Blood and saliva samples and kept them for 1 month at room temperature.. is RNA preserved?
04 June 2017 7,471 1 View
Since heparin inhibits reverse transcriptase, heparinase I treatments prior to qRT-PCRs are recommended. NGS also uses reverse transcriptase, so I was wondering whether anyone has tried doing a...
31 May 2017 533 1 View
We usually use this kit which has a qia shredder column to lyse the cell before transferring the lysate onto another column for further RNA purification. However we have run out of this qia...
23 May 2017 1,013 3 View
Hi! I am need to isolate mRNA from cells for MS analysis. I used Trizol/Chloroform RNA extraction and then one kit for poly(A) selection for the mRNA isolation but I am looking for other methods...
22 May 2017 3,232 1 View
I am dissecting the Hippocampus in cold PBS and homogenizing directly in TRIZOL, but despite that I am getting RQN close to 4.
18 May 2017 5,148 6 View
Taking 5 ml blood i should get a good yield of DNA as the birds have nucleated rbc can anyone please tell me a proper phenol choloroform method to solve it
18 May 2017 10,099 2 View
I have extracted RNA from avian blood using TRIzol from ambion. After a 3M sodium acetate / ethanol precipitation, the RIN number reads >8 on the bioanalyzer. I want to treat the RNA with TURBO...
18 May 2017 3,312 5 View
Hi AllI am using Trizol LS reagent for RNA and DNA extraction from Grape-vine leaf tissue, but I couldn't find any RNA and DNA. I found big pellet and a bit it is hard to dissolve. I check the...
16 May 2017 5,531 3 View
I am looking at alternative options for quantifying bacterial viability in addition to colony plate counting. I have a mixed species bacterial culture that may also contain epithelial cells. I...
14 May 2017 7,716 5 View
I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...
04 May 2017 6,197 4 View
The upper aqueous phase appears in the bottom of the tube.
02 May 2017 8,893 8 View
Has any one tried RNA extraction from ,72 hours old bone marrow sample EDTA blood
01 May 2017 6,080 5 View
Hello everyone, I needed to know if anyone knows this fact, or has had trouble expressing exogenous genes in Arabidopsis using the 35S CaMV promoter (2X35S more accurately) without putting a...
30 April 2017 8,182 5 View
Hi, I was wondering, if it is possible to extract total RNA and DNA from insect which have been fixed or stored in 70% ethanol at RT and some specimen at 4 degree. The isolated total RNA would be...
29 April 2017 4,839 3 View
I'm having problems with my RNA extraction with Trizol. Basically, I'm growing my plants in semi-sterile pouches (basically a plastic bag with a filter paper that allows the shoot to go up and...
26 April 2017 1,089 6 View
I extracted total RNA from frozen PBMCs using Norgen's total RNA extraction kit so that I could retain microRNAs for a small RNA sequencing experiment. The tapestation trace is bizarre! The...
26 April 2017 2,926 3 View
Hello All, I'm wondering what's the best plant total RNA extraction kit to use, if the down stream assay will be qPCR? Also this is for corn leaves and roots. I've been using the Spectrum (TM)...
25 April 2017 880 2 View
I want to preserve breast tissues for RNA extraction later ( after 2-4 months). is it possible preserve those tissues at -80 or at -20 without preservative reagents ( such as RNA later or...
23 April 2017 9,290 4 View
I am working on the next generation sequencing of EAV from horse semen samples. As my attempts of isolating virus on RK13 cell cultures were unsuccessful I have decided to use RNA material...
19 April 2017 2,339 3 View