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Questions related to RNA Extraction
Hello everybody, I have a question about the conservation of fish samples for a RNA extraction.More precisely, I have some juveniles and I need to extract good quality RNA from head and gonads. To...
24 November 2016 5,879 3 View
Should I keep repeating the extraction process. I am using Easy-REdBYF total RNA extraction kit. Are there any tips on improving conc.? 260/230 value is between 1.5 & 1.9.
24 November 2016 2,170 7 View
I am using Sigma DNase I (AMPD1) where I add 1 ul of each component(DNAse1, buffer and stop solution) to 8 ul of RNA in water (below is the link to the...
22 November 2016 3,327 2 View
Is there a lab researcher to do a RNA extraction from white blood cells to make a cDNA sequencing possible? I am trying to find out whether some unknown variants might cause changed protein function.
21 November 2016 4,630 2 View
Usually we do it with 6 embryos. Now for a lot of reasons we need to do RNA isolation and even Western blot with only a single embryo. Please kindly guide me with your notes. Thanks
16 November 2016 785 4 View
I've recently done an RNA extraction from mouse liver progenitor cells- on running my RNA samples on a gel, I noticed a distinct band above the 28S. Any ideas on what it may be? I don't think it's...
15 November 2016 8,961 2 View
There are papers that suggest that gDNA can be isolated from paxgene blood RNA tube. I am using Paxgene RNA extraction kit for extracting RNA. We are interested in isolating DNA also for some...
15 November 2016 1,954 3 View
Can you help me for RNA extraction from saliva sample? QUAgene company has a kit for RNA extraction from saliva but it is very expensive. I want another kit or method for RNA extraction from saliva .
14 November 2016 2,062 3 View
Hello. I am trying to optimize the culture conditions for a bacterial isolate that produces pigment on nutrient agar but not in nutrient broth. I have tried to increase the incubation time for...
09 November 2016 1,120 7 View
I am working with low (and expensive) amounts of tissue. Following a TRIzol extraction, I have got poor quality RNA and a low amount. I think that the main issue would be following the isopropanol...
05 November 2016 9,522 3 View
I am doing an IVT of guide DNA sequences. My template DNA is ~120 bp, but after the T7 promoter, there are only ~100bp. But after I do the IVT and run on a gel, I get two separate bands. I am...
04 November 2016 1,572 5 View
Extraction of RNA for detection of dengue and zika virus using RT-PCR, as what I know, RNA degrade easily. Is it necessary for us to extract from fresh sample/sample that kept in -80*C?
02 November 2016 4,945 2 View
Hi all, are LB broth and Nutrient broth minimal or complex media? I am following the QIAGEN protocol for the RNA extraction from bacteria and the protocol for the preparation of lysates is...
02 November 2016 6,196 3 View
I am using RNeasy plus mini kit for extraction of RNA from E. coli. I am following the procedure as written but when I run my eluted sample on the agarose gel to see the RNA integrity (which...
01 November 2016 5,472 7 View
I am using laser capture microdissection to remove a few cells (Between 300-700) per tube, and I was wondering how I can condition a silica column to get good yields of RNA from the lysate? I have...
31 October 2016 7,266 5 View
Hi, so there's my problem. I have done a RNA extraction, ran a gel with it and dosed it. The picture of the gel is after my message and for all my samples, the concentration was between 1250-1550...
28 October 2016 4,505 4 View
I have isolated RNA from keratinocyte cells using Trireagent from SigmaAldrich. With varying concentration, low 260/230, and a spectra that matches phenol contamination, I suspect.......
20 October 2016 6,964 2 View
Now i´m working in RNA isolation of the half part or all the maxilar palate mucosa of BALB/c mouse, with the aim to measure the gene expression of different cytokines with RT-qPCR. But my problem...
11 October 2016 6,314 5 View
Dear Scientists & Researchers, I am going to amplify a specific gene from a (-)ssRNA virus. I have collected several infected tissues and extracted their Total RNA with RNX-Plus solution. Then...
10 October 2016 5,360 3 View
RNA extraction from low volume of blood samples...
10 October 2016 9,332 3 View
In practice I want to do a Mixed Lymphocyte Colture and after incubation I want to isolate only the MSC to extract their RNA. The problem is that MSC are plastic adherent, but also the monocytes...
07 October 2016 4,659 1 View
I am dealing with Candida RNA isolation from TRI reagent (used qiazen kit for extraction).I am really concerned about the quality of my RNA. According to Nanodrop reading my yields were ~100 to...
04 October 2016 8,223 3 View
Is their any protocol or reagent by which I can store mice colon tumor samples at -20 or 2-4℃ and latter extract intact RNA and protein.
03 October 2016 1,808 3 View
I am trying to find out the protocol to extract RNA from the caecum of the chicken. Thinking logically, the condition of the caecum (or even any parts of the intestine) provides a great source of...
03 October 2016 5,859 1 View