Contact experts in RNA Extraction to get answers
1,499 views 707 posts
Questions related to RNA Extraction
I use QuiaCube automated RNA extraction from PB MNCs??
27 March 2017 9,720 4 View
I am working on stool samples stored under glycerin , but there is an excess and mixing of glycerin with the stool sample , this lead to lowering the RNA yield due to sample dilution, How can i...
15 March 2017 1,276 1 View
Hi there, I am extracting RNA from environmental samples like soil. I have a three stage protocol. First I use a Chloroform/Phenol based extraction according to Toewe et all, then I am using a...
14 March 2017 795 7 View
I'm extracting total RNA from yeast with MasterPure- Yeast RNA Purification Kit - Epicentre but when I run the sample in a gel I can see plasmidic DNA left even after treatment with DNAse I. Is...
10 March 2017 7,226 3 View
I'm using the Ambion RNAqueous Micro Kit to extract RNA from cells in different pathways. I use laser capture microdissection to extract the cells. From one pathway, I normally collect 8-50 cells....
08 March 2017 8,572 4 View
Hi all, in our lab we would like to switch from Mercaptoethanol to TCEP as a reducing agent for RNA protection during isolation. What concentration do you use? I only found a protocoll from...
02 March 2017 4,491 2 View
Hi, Is there a method to extract RNA of both gram positive and gram negative bacteria in their dual-species culture? I want to perform transcriptomic analysis, so I need RNA sample with high...
02 March 2017 8,047 2 View
Can anyone help me how to increase RNA yield from exosomes ? I was able to get RNA concentrations of only around 30-40ng/ul per sample from my exosomes and I would be happy to hear any suggestions...
28 February 2017 7,674 7 View
RNA extraction
28 February 2017 5,893 2 View
I have RNA samples stored at -70º, that have been extracted from animal blood, excrements, urine and saliva about 3 years ago. I wonder if this RNA samples are stable to use in a RT-PCR assay.
27 February 2017 3,786 6 View
HI. I isolated RNA many times, and suddenly something weird happened: I used TRI-reagent 0.5 ml, and stored the tubes on the tubes rotator for 10 min. I added 50 uL BCP, waited 5 min, and...
23 February 2017 2,276 5 View
Hello, What's the best way to disrupt a tissue without affecting too much to the RNA? I will use RNeasy for RNA extraction, but before I have to homogenize my sample, and I think using a needle...
23 February 2017 982 3 View
I have gotten a low RNA amount from human dendritic cells using RNeasy kit from Qiagen, such as 11ng/ul and I need to perform a qPCR assay from these samples. Or trizol still is the better way to...
22 February 2017 1,039 3 View
I used Qiagen mini kit for isolation of RNA from mouse bone marrow cells and spleen tissue. The samples were in stored 4 days before at -80C. The 260/280 ratio in 4 different samples is 1.532,...
21 February 2017 9,417 2 View
Has anyone isolated tumor RNA from cell lines for use in cell culture? In all the papers I've read I can see no mention of isolating the RNA in any more sterile manner than is required for any RNA...
21 February 2017 8,670 3 View
i want to extract RNA from the cornea. kindly suggest me a simple method for frozen cornea.
19 February 2017 6,517 5 View
Hello everybody. I'm doing DNA extractions from rizobacteria. The problem is the 260/230 ratio. I have both low and high ratios (eg. 0.76 or 3-4.09). When I search information in Thermo Scientific...
16 February 2017 5,393 5 View
To guarantee good results and purified yield
14 February 2017 733 4 View
I am trying to precipitate RNA isolated from Silkworm larval midgut using absolute and ethanol 3M NaOAc (for storage). The protocol for this has been previously standardized in the lab. It...
11 February 2017 1,527 2 View
Hey everyone! I need some advice for the determination of the mutant adult zebrafish I generated (besides DNA Sequencing). I want to use Western blot to check if the protein is really gone. I...
10 February 2017 191 1 View
I would like to keep my RNA and cDNA samples (from blood and chicken tissues) for long time, Could anybody please suggest me about how and how long for their sample preservation? Many thanks in...
08 February 2017 3,920 5 View
Dear fellow scientist, For RNA isolation I use Trizol, however is pH of Trizol between 4- 6 which allow DNA to be retained in the organic phase and interface, leaving the RNA in the aqueous...
26 January 2017 8,339 6 View
I am preparing to do a maxi-prep and have a pellet bacterial cells that have been at -20 for about 2 months.
05 January 2017 2,322 3 View
Hi all, I extracted RNA from bacteria and I run an agarose gel on these samples. How do I recognize if I have RNA and not DNA? I have three bands (in attachment) and I am not sure about what they...
22 December 2016 8,048 13 View