Contact experts in Nucleic Acids to get answers
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Questions related to Nucleic Acids
Is there any simple method/ test to check this?
20 October 2017 1,444 3 View
I'm looking to the best method to extract both RNA and DNA from the same sample (mice brain tissues). Sometimes we just separate the tissue in the 2 hemisphere, but this is not the case. I tryed...
10 October 2017 5,288 3 View
10 October 2017 9,491 4 View
I have induced dsRNA in bacteria following most reported protocol in literature, but I am presently stock at cleaning the nucleic acid I want to eliminated RNA and DNA so I would be left with...
10 October 2017 8,576 1 View
As routine usage of ethidium bromide in lab for staining the nucleic acids from decades ago,why we are not getting the stained nucleic acids could not get modify by the dye. We have frequently...
12 August 2017 9,920 6 View
A phage is not a cell. Normally it just has a protein shell encapsulates nucleic acid. So what will happen, after a phage 'dies' or 'is inactive'? Will the particle still keep its intact...
08 August 2017 8,126 7 View
xylene cyanol blue is a dye for nucleic acids
09 June 2017 7,392 2 View
Is it possible to cut a band from an agarose gel and purify it for subsequently creating a stock solution for qPCR? I use "gelgreen nucleic acid gel stain" (Biotium), for the agarose gel. Now I'm...
10 May 2017 3,669 2 View
I am working on nucleic acid (NA). I have to add K(+) ions to study its behaviour. I want to keep the coordinates of NA molecule and K(+) fixed & allow water molecules to equilibrate. This...
04 April 2017 1,115 2 View
04 April 2017 8,796 2 View
Some proteins have secondary, tertiary or primary structures. Even some of the nucleic acids have the same phenomenon. what is the importance of these structures in strength and flexibility? Which...
11 November 2016 577 5 View
My protein (overexpressed in E. coli) is co-purified bound to nucleic acids (High A260/A280 rate). I want to get rid off DNA adding DNAse to the lysis buffer prior to chromatography purification...
11 November 2016 9,877 2 View
10 November 2016 1,562 2 View
I am wondering whether I can modify any step to increase yield, thanks a lot!!!
10 October 2016 2,707 0 View
I want to study the change in profile of nucleic acid associated proteins from bacteria.
23 September 2016 4,003 2 View
I have been struggling with purification of a highly basic protein. This protein is His-tagged. We get pretty good purity from Ni column. The pooled Ni column fractions is in 500 mM NaCl, ~ 200 mM...
18 August 2016 2,961 8 View
Read 3 answers by scientists with 1 recommendation from their colleagues to the question asked by Rama Rao Damerla on Aug 22, 2016
08 August 2016 3,637 3 View
So this may be a dumb question, and I apologize in advance. So I was asked today by my principal investigator "what do you call the base immediately next to the terminal base in...
08 August 2016 4,342 2 View
During a viral diagnosis for a complete virus particle, its antigen, or its nucleic acid, what portion of the patient sample being detected is the complete virus vs. pieces of its antigens or...
22 July 2016 6,672 2 View
I am interested to know the chemical composition of ExoFect. Whether this can be used to load small molecules too? Any patent or detailed sheet about the composition?
14 July 2016 9,945 3 View
07 July 2016 3,340 4 View
I was referring to SYBR Green I (SG), which is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology during the performance of Restriction enzyme digestion.
07 July 2016 7,665 4 View
07 July 2016 7,505 2 View
How to preserve the nucleic acid for long-term?
07 July 2016 5,791 2 View