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Questions related to Nucleic Acids
About the environment, it refers to ion species and amount, other biopolymers and so on.
05 May 2014 3,594 4 View
I attempted to immobilize a biotinylated nucleic acid polymer on SA sensor chip using a default immobilization method (Biacore). The immobilization resulted in negative (!!) immobilization levels....
05 May 2014 8,195 2 View
Because chromatin dots of malaria parasites contain nucleic acids, my expectation is that they attract the positively charged dye of the stain(MB) and get blue stained. But they normally appear red!
04 April 2014 4,457 7 View
We can make lots of modification on 3' or 5' of the DNA oligonucleoties. Say, I add a modification group to 3' of oligonucleotide (such as thiol, amino, biotin, phosphate, or a fluorophore) ....
04 April 2014 1,185 9 View
There is no PDB file available in the protein data bank, so I have to create the PDB file. Can anyone help me please?
04 April 2014 1,446 2 View
In autodock DNA has much more torsion than a protein. So selection of flexible residue is important. What are the flexible residues for DNA and RNA?
04 April 2014 5,801 4 View
Please share your knowledge on the faith of oxidized nucleic acid bases. Fast yet extensive PubMed browsing gave no positive results. Most papers deal with processes that lead to free oxidized...
03 March 2014 9,989 1 View
I have two complementary oligonucleotides (about 25 nt). How long will they hybridize into dSDNA in a certain buffer at room temperature? Are there some differences between immobilization and...
03 March 2014 1,529 2 View
In an informal discussion on plasmids the above question popped up. Views for and against were put forth. I thought I should seek views of scientific community on the topic of general interest to...
03 March 2014 7,254 7 View
From powder XRD we can measure the value of basal spacing. The value of basal spacing should be increased for the intercalation process. So, for liquid samples is this theory applicable or not?
03 March 2014 1,050 2 View
Can someone tell me if Qubit is really better than Nanodrop in nucleic acids quantification, even for small concentrations?
01 January 2014 9,905 39 View
I would like to understand how artificial DNA differs from natural DNA in the area of combination and replication. Can XNA carry non typical characteristics such as magnetism, photo sensitivity?
12 December 2013 8,327 2 View
I was wondering how to calculate the isoelectric point of a given length of DNA. Let's say a 20 bases DNA single strand. Could anyone help on this? Thanks a lot.
11 November 2013 3,822 2 View
Other than the usual method of adding divalent cations of small ionic radii, is there any better method? I want very clean background.
11 November 2013 7,103 0 View
Can anyone give me some suggestion, what kind of PCR techniques can I use to quantify nucleic acid/ PCR product, if only normal thermal cycler is available in my lab?
11 November 2013 8,894 5 View
Using a thermal melting study, is it possible to evaluate thermodynamic parameters easily?
11 November 2013 3,346 3 View
We have tried some gels with undefined bands, but sometimes the gels are ok. Does anyone have a bad experience with this nucleic acid gel stain?
10 October 2013 1,404 7 View
Universal Nucleic Acid can form hydrogen bonding with all nucleic acid bases, G A C T(U). I want to make it and that is one of my dream. And, how about to use Janus base, which has two faces to...
10 October 2013 3,482 0 View
I am trying to optimize my EMSA conditions. What is the amount of proteins I should try first?
10 October 2013 414 0 View
I want to monitor viral lysis by qRT-PCR, but free RNA in the viral prep prior to lysis inflates the copy number amplified.
10 October 2013 6,854 2 View
10 October 2013 4,908 2 View
Can oligonucleotides be labeld with Cy5 on both ends?
09 September 2013 2,312 4 View
How does DNA polymerase discriminate during replication between dNTPs and NTPs when the presence of NTPs is ten-fold higher in the cell?
09 September 2013 3,936 3 View
09 September 2013 1,912 3 View