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Questions related to Nucleic Acids
I plan to use next generation sequencing of RNA and DNA on clinical samples of whole blood (EDTA), after storing them for weeks-months at 0-80C what is the best method to store whole blood to...
10 October 2015 7,391 4 View
Does anyone have experience using Trizol or Trizol LS to extract total nucleic acid from stool? I need to use these reagents in order to remove samples from a BSL-4 containment laboratory. Would I...
10 October 2015 4,476 6 View
In the isolation of genomic DNA by silica gel membrane column method why only nucleic acids bound to the silica gel ,Can u tell me why not amino acids attach to the silica gel membrane ?
09 September 2015 4,428 2 View
I am doing some research on acridine orange (AO). I've found out that the emission wavelength of AO depends on whether it binds to double-stranded or single-stranded nucleic acids (DNA/RNA), due...
09 September 2015 6,283 2 View
02 September 2015 3,560 2 View
is there any software to calculate the Kd value of nucleic acid - nuclei acid complex in a set condition. i want to compare it with the Kd value of aptamer-nucleic acid complex.
08 August 2015 2,568 2 View
Hi everyone, in most of my lab experiments during RNA isolation we use animal tissues rather than plant tissues. Generally we know animal tissues have more protein production, high amount of...
08 August 2015 7,281 3 View
I saw a protocol for the extraction of Nucleic acid directly from agarose gel by phenol digested electrophoresis, the procedure involves: cut the band you want, put it in a EP tube, crush by a...
08 August 2015 9,785 7 View
what is the use of chaotropic agents in spin column-based nucleic acid purification?
08 August 2015 1,126 4 View
In our setting, we have only one set of equipment's for molecular work which was given for viral gene detection; shall I use the same equipment's in same molecular biology laboratory for the...
08 August 2015 5,919 4 View
My objective is to have a reading of DNA content in my RNA samples before and after DNase treatment....
08 August 2015 362 5 View
Please provide evidence for your answer.
07 July 2015 5,504 0 View
I have read one journal "Characteristics and Applications of Nucleic Acid Sequence-Based Amplification" by Deiman et al., 2002. Is it possible to "upgrade" NASBA so it can be used to amplify...
07 July 2015 6,363 2 View
Can I perform SDS-PAGE for seperation of Nucleic acids instead of Agarose Gel Electrophoresis?
06 June 2015 2,140 3 View
Na/k acetate is used to neutralize the nucleic acid following alkaline lysis where Na/K binds with the nucleic acid followed by alcohol precipitation. Na/k exist with DNA following elution or we...
16 May 2015 3,000 3 View
05 May 2015 6,624 4 View
Hello, I have been using Polyethylene Imine (PEI) on a regular to precipitate DNA from crude bacterial extracts in order to get rid of the DNA on my way to purify proteins of interest. I am...
05 May 2015 8,010 4 View
I am interested in getting your feedback on how this new emerging technology will disrupt current approaches to getting nucleic acids proteins and large plasmids like those in Cas9 into primary...
05 May 2015 10,004 0 View
Hi everybody, I am wondering whether anyone is aware about the intracellular levels (molarity) of guanine in normal, healthy cells. I would be particularly interested in the levels measured in...
23 April 2015 3,022 1 View
After the dsDNA nucleic acid enter the nucleus of the host cell it is transcribed from host DdRp II to form 2 strands of mRNA complementary to the 2 DNA strands of the virus nucleic acid. Does...
12 April 2015 613 2 View
04 April 2015 395 1 View
Why Proteins are mostly separated by Polyacrylamide gel electrophoresis and nucleic acids in agarose gel electrophoresis.?
04 April 2015 1,838 4 View
Hello, Please anyone explain to me, how I may use the probes information available on probebase online database and how I may use the probe sequences (like primers on probebase) and amplify the...
04 April 2015 9,722 3 View
For proteins vertical gel electrophoresis is preferred while for nucleic acids horizontal electrophoresis is preferred, Why ?
04 April 2015 8,167 3 View