How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Transmembrane Protein 3-D Coordinate File Preparation Question?

Hello I hope you are all doing well.:) Several transmembrane Pannexin1 3-D coordinate files have been submitted to the PDB, however, I believe all are missing coordinates in some of the Pannexin1...

11 August 2020 4,875 3 View

What are the best metrics for evaluating the quality of non-spherical clusters?

Hi. I'm dealing with clustering of data were the resulting clusters are, in general, non-spherical. Some of them are not convex. What are the best internal metrics for evaluating these kind of...

27 March 2020 5,076 2 View

How can I separate hematite from bornite in a powder?

17 March 2020 570 3 View

How can I access the total number of integration points present in my model using a vumat in Abaqus ?

Hello, I'm using a vumat in Abaqus and i'm storing data associated to each Integration Point present in my model. For the time being, I scale my array to a given large size, say M(10000). I...

08 January 2020 9,622 3 View

I have tried to clone dcas9 into pET22b vector for the past 1month now. And I do'nt always have any colonies. Anyone cloned dcas9 into pET vectors?

I always have pure insert and vector DNA in the region of 1.7-2.0. I use restriction enzymes sall HF and xhol and perform ligation overnight for 16hrs and then do transformation in 10 beta...

30 September 2019 7,178 2 View

Please, has anyone worked on sgRNA labelling? I am finding it difficult to get protocols to label with alexa dye and Qds.?

How can I label single guided RNA (sgRNA) with Alexa 488 dye and quantum dots (QDs)? I will appreciate any paper recommended or detailed protocols.

30 September 2019 6,873 1 View

How do I stop TEM samples being destroyed under electron beam?

The samples that I make for TEM contain cells. I need them to be 70-90nm (no higher) to be able to see them clearly and do EDX as well. However, They begin to rip and flex under the beam even at...

19 August 2019 2,339 1 View

Correct statistical analysis for intervention study pre and post test?

I have ran a study comparing the Daily Mile and HIIT in children to see which is best for improving cardiovascular endurance (Beep test) and anaerobic power (20m sprint). Scores on both test...

29 April 2019 7,457 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View