Virus particle, its antigen, or its nucleic acid when in the blood stream precentages?

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What are the research gaps for telematic technologies for road pavements optimization?

what can be the undiscovered benefits of applying telematic technologies for road pavement maintenance, incident reduction and environmental protection?

29 June 2024 7,507 1 View

Why am i getting the human verification when i search for certain topic?

most of the time when i search fo certain topic i found the human verification respond and nothing change there after

19 March 2024 6,870 0 View

How to handle twins in birth order analysis?

Hello! I have a methodological question and I hope someone can help me. I collected data via an online survey with a sample size of 2600 (N = 2600). Initially, I aimed to examine the effect of...

26 February 2024 6,911 4 View

FfTK Calc Bonded Tab ORCA gbw file not recognized INQUIRY?

Hi RG Community I had input all the appropriate files, however, received an Action halted on error! My ORCA generated .gbw QM Output file was not recognized (please see screenshot attached). I...

15 February 2024 2,553 1 View

How long would it take a biofiltratration sysytem in fish culture to complete the nitrogen cycle?

Typically, it is said that biofilter by their nature allow the settling of bacteria beneficial to the treatment of fishwaste. Fish culture though is known for producing high amounts of ammonia in...

28 January 2024 2,398 3 View

Why do eastern European nations seem to have a significantly lower all-cause mortality rate than the UK, Canada, and the U.S.?

From what I can tell by searching the "Your World in Data" website, eastern Europe seems to have an expected lower than average all-cause mortality rate than the western first world nations, who...

04 January 2024 7,066 0 View

Topology Validation Inquiry within an .str File via CGenFF Inquiry?

Hello RG Community:), I have a ligand exhibiting penalties between 10-50 within a .str file generated via a CGenFF, the generated .prm file does not exactly match the ligand atoms nor the closest...

25 December 2023 4,364 1 View

Alphafold Monomer versus MODELLER Oligomer Loop Modeling Inquiry?

Hi I had modelled a heptamer transmembrane protein missing 10-residues within the extracellular loops via MODELLER constraining the known residues as a heptamer (when MODELLER modeled as a monomer...

08 December 2023 4,512 0 View

AlphaFold Multimer Loop Modeling Inquiry?

Hello towards the above topic, I successfully loop-modeled a transmembrane heptamer protein (426-residues per monomer) exhibiting 7-extracellular loops each missing 11-residues via MODELLER via...

03 December 2023 9,528 2 View

Need a simulation tool to generate synthetic data 🙏?

Need a simulation tool to generate synthetic data Specialized engine simulation software (for control and diagnosis) how and what to do 🙏?

16 November 2023 4,128 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View