I'm looking to the best method to extract both RNA and DNA from the same sample (mice brain tissues). Sometimes we just separate the tissue in the 2 hemisphere, but this is not the case.
I tryed the TriReagent extraction, but it worked only for RNA.
Thus I'm looking to a commercially available kit that give the best results in terms of quantity and quality. I would expect nucleic acids extraction to have lower yield, but enough good quality (compared to TriReagent).
What would you recommend for mice brain tissue based on your experience?
If necessary, nucleic acids clean up (e.g. DNAse digestion of contaminating gDNA in the RNA sample) can be done separately, if good yields are achieved during extraction.
Thanks in advance for any help!!
I would use Qiagen Kits (usually for genotyping from mouse tail tissue) for extracting DNA from mouse tissue
Thank you for the answer, but it does not fit my needs.
I need to extract both RNA and DNA from the same sample. Thus I would loose the RNA with the kit you suggest me.
Qiagen provides AllPrep kit for RNA/DNA extraction. But also other companies have similar kit.
Thus my question is: which company provides the best kit in term of quality and quantity of extracted nucleic acids from brain tissue? Does anyone have some experience with this?
Right, I see. There should be an Ambion kit for simultaneous extraction of RNA and DNA from soft tissue.
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