Nucleic Acids

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Questions related to Nucleic Acids

Which nucleases can degrade G-quadruplex ?

I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...

01 November 2018 1,204 2 View

Are nucleic acids present in coconut water?

Coconut water or the liquid endosperm of Cocos nucifera L. is an interesting biological material. It has been widely used in growth media for plant tissue culture (1). TULECKE et al. (2) have made...

10 October 2018 778 0 View

Categories of Ligation Reaction?

ligation is the joining of two nucleic acid fragments through the action of an enzyme

10 October 2018 855 1 View

What are the chemical reagents used in testing Nucleic acids and lipoproteins?

Detail procedures for testing nucleic acids (DNA & RNA) and Lipoproteins(HDL and LDL)

10 October 2018 399 0 View

What is Fermi Paradox?

What are the chances that Alien Life is composed of nucleic acids.

10 October 2018 6,672 0 View

What RPA amplicon to buffer ratio is suitable to detect RPA products using Abingdon Health PCRD Nucleic acid detector sticks?

I am currently developing RPAs for the detection of flaviviruses, however I am struggling a bit with determining the minimum detection limits of my assays. I performed ten-fold dilutions on...

10 October 2018 2,617 1 View

Is it possible to have a simple and unloaded Nucleotide in the Nucleic acid?

first of all I should apologize for this low level question and then: when we say Nucleic acid ,could we contain histones in this structure? if we could,for the answere of the first question,we...

10 October 2018 1,438 3 View

Melting temperature (Tm) of G-quadruplex DNA?

I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...

08 October 2018 4,015 0 View

Wondering how to enrich or concentrate phages or viruses for community phage/virus nucleic acid extraction and that too without using host?

We all know about the specificity of viruses or phages and I guess that would specially limit their enrichment to certain groups. Suppose I have a sediment or sewage sample and I want to enrich...

26 September 2018 1,123 14 View

Why does the ladder band appear in genomic DNA electrophoresis ?

Hi everybody, I am using the High Pure Viral Nucleic Acid Kit (Roche) for extraction genomic DNA from blood (buffy coat). The DNA concentration is quite good (50-100 ng/ul) and the A260/A280 ratio...

09 September 2018 2,464 5 View

Wondering how to enrich or concentrate phages or viruses for community phage/virus nucleic acid extraction and that too without using host?

We all know about the specificity of viruses or phages and I guess that would specially limit their enrichment to certain groups. Suppose I have a sediment or sewage sample and I want to enrich...

09 September 2018 6,580 15 View

How does the AMOEBA polarizable force field for nucleic acids impact protein MD?

Has anyone tried the new AMOEBA force field for nucleic acids (Zhang et al, 2018) on protein-DNA or protein-RNA simulations? I'm curious to see how the torsion parameters manifest in simulating...

09 September 2018 8,956 1 View

Is there innate immunity in the mouse zygote?

RNA can elicit innate immunity in cultured cells during CRISPR editing, a fact that further supports the growing use of Cas9-synthetic gRNA as an RNP for superior editing in mouse embryos. But,...

09 September 2018 9,195 0 View

Why does the ladder band appear in genomic DNA electrophoresis ?

Hi everybody, I am using the High Pure Viral Nucleic Acid Kit (Roche) for extraction genomic DNA from blood (buffy coat). The DNA concentration is quite good (50-100 ng/ul) and the A260/A280 ratio...

04 September 2018 3,552 4 View

MBP fusion protein not binding to amylose beads?

I have a MBP fusion protein that is not binding to amylose beads. I have already tried adding glucose to the media (although I did not add it to my starter culture) and I am not using detergents...

23 August 2018 5,944 7 View

How can we able to discriminate nucleic acids on agarose gel?

Generally agarise gel electrophoresis is a technique used to separate the DNA bands with the help of electricity

08 August 2018 4,890 1 View

Anyone knows a company that performs biomass measurement from plant tissues?

Dear all. I would like to perform a biomass analysis of plant tissues. I need, basically, to determine in a quantitative way the amount of starch, cell wall, nucleic acids, proteins, lipids,...

08 August 2018 6,272 3 View

Consequences of using Tris-EDTA buffer instead of Tris-HCl for DNA elution?

I am trying to isolate isoforms of plasmids from agarose gels using a nucleic acid purification kit, and I have been playing around with the my choice elution buffer in order to see if I can...

19 July 2018 5,905 2 View

Can we attach nucleic acit to aptamers?

as you know aptamer mechanism and i wonder that if we attach any nucleic acids ,that are mRNA, miRNA or any gene..., to aptamer and transfect to bacteria or cell, what is the conclusion or if you...

07 July 2018 8,489 2 View

What is SYBR gold?

SYBR Green (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in RT q PCR in molecular biology.

07 July 2018 8,669 1 View

How to build peptide nucleic acids ?

Hi all, Are there any software which can build peptide nucleic acids with specific sequence ?

02 July 2018 2,829 5 View

How can I get RNA spectra in MALDI ?

I am using AB Sciex 5800 TOF/TOF MALDI platform for analysis of RNA. For that, initially I am trying to standardize with 20-mer DNA primers by using 3-HPA and THAP as matrix but I couldn't get any...

11 June 2018 6,046 7 View

Is agitation necessary during IHC antibody incubation for 400 um hippocampal slices?

I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid...

10 June 2018 9,885 3 View

How can I get RNA spectra in MALDI ?

I am using AB Sciex 5800 TOF/TOF MALDI platform for analysis of RNA. For that, initially I am trying to standardize with 20-mer DNA primers by using 3-HPA and THAP as matrix but I couldn't get any...

06 June 2018 6,054 8 View

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