How to purify all the nucleic acid (DNA and RNA) associated protein from bacteria?

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In deuteration of aryl halides with CD3OD, beta deuteride elemination (C-D bond cleavge)is faster than weaker O-D bond? why ?

Compare to C-D bond O-D bond is weaker. According to catalytic cycle (attached below) O-D bond cleavage is not occur? Could please explain the reason? Reaction condition: Pd(OAc)2/ Catacxium...

23 January 2023 9,718 3 View

Hello Friends, How much % of impurity need to be generated in forced degaradation (FDS) of Biomolecules?

Bio molecules are less stable compared to Pharma drugs. Since proteins are notorious for stability many impurities gets generated on the stability. If impurities are not available, we need to...

22 March 2018 6,649 3 View

How to detect somatic mutation from blood sample? I also wanted to know how blood is collected for normal and disease samples?

I know about collection of tissue samples from normal as well as disease tissues. Here I wanted to know how the same is collected in terms of blood? Also, how detection of somatic mutation...

10 July 2017 1,217 2 View

Availability of Somatic Mutation Gene Panel in Breast Cancer?

Is there any somatic mutation gene panel for detecting breast cancer? I already know there are Germline mutation gene panel for Breast Cancer

09 July 2017 5,513 1 View

How to separate G and D' peaks of highly defective Graphene or vertical graphene nanowalls in Raman spectroscopy.

In Raman spectroscopy of Graphene or vertical graphene nanowalls,with increase in defect density, the D' peak intensity increases, shift to lower wave numbers and finally merges with G peak. I...

21 June 2017 3,779 2 View

Flow sort fixed permeabilized cells for RNAseq - protocol?

Hello fellow researchers, have you ever performed RNAseq from flow sorted fixed permeabilized cells after intracellular staining.

15 May 2017 4,547 1 View

What is the optimum irreproducible retention time of the peak in HPLC?

I am doing reverse phase HPLC using C18. Keeping the flow rate and composition of solvent constant, I should get my target peak at 14 min, but I am getting the peak sometime at 14 minutes sometime...

27 June 2014 4,471 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View