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Questions related to Nucleic Acids
What is the cause of HIV sero-negativity and HIV PCR positivity among some babies born to HIV infected mother while the babies are under ART? Should one continue ATR despite HIV antibody...
13 October 2014 5,005 8 View
I want to perform a molecular dynamics simulation for an unnatural base pair inside DNA. I have done the parameterization of individual base pairs, terminating the pair-sugar junction by methyl...
10 October 2014 8,591 7 View
I am experiencing a contamination problem in my DNA extracts. I did DNA extraction from water samples using phenol/chloroform. However, I do get a shoulder/high peak at 230 nm whereas there is a...
10 October 2014 10,091 6 View
Specifically, does ethidium bromide bind with equal efficiency to single stranded DNA as to double stranded? Is there a good resource for learning about the chemistry behind EB? THANKS!
10 October 2014 2,730 4 View
10 October 2014 7,343 9 View
We have a collection of inoculated MicroGard filters (see link) and we would like to extract DNA from those. Thus, we are looking for a good procedure to do so. We have experience in extracting...
09 September 2014 4,102 0 View
I want to prepare slides of DT-40 cells for a TUNEL assay. My question is, is there a protocol for preparing them and fixing them so they can be stored at -80 degrees for about a week?
09 September 2014 4,937 2 View
Phalloidin staining on the test and control cells show difference in organisation of polymerised filamentous actin. Can we show this difference in the extent of polymerisation or in the amount of...
09 September 2014 7,603 5 View
nucleic acid production in salvage pathway
25 August 2014 1,429 2 View
RNA Analysis
08 August 2014 8,435 4 View
PEG DNA precipitation
08 August 2014 978 2 View
Would it make any trouble if I we use phosphate buffer instead of TE buffer in diluting Nucleic acids? If I am correct, phosphate buffer is used typically to maintain the viability of live cells....
08 August 2014 7,142 9 View
08 August 2014 2,483 2 View
I have recently isolated 8 bacteriophages and I am in the process of characterising them. Does anyone know of a simple method to determine what genome they have?
08 August 2014 2,086 5 View
06 August 2014 4,860 4 View
I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...
18 July 2014 5,589 2 View
what does TCID50 tell us?
17 July 2014 9,160 3 View
Regarding viral nucleic acid maintentance
09 July 2014 2,642 5 View
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07 July 2014 1,835 0 View
07 July 2014 1,501 4 View
07 July 2014 1,755 6 View
07 July 2014 9,806 4 View
My hypothesis: Since nucleic acids has a peak absorbance at 260nm wavelength and proteins at 280nm, the ratio of greater than 2.0 is considered relatively pure RNA. Twist: However, literature...
06 June 2014 1,391 4 View
I've been looking for a way to understand how to determine the molarity of nucleic acids in numbers of nucleotides and in numbers of molecules. For example: if I get the size, in base pairs, of...
05 May 2014 1,005 7 View