I am wondering whether I can modify any step to increase yield, thanks a lot!!!
We are receiving blood samples that sometimes we can't make to stain them freshly for FACS. Is it better to keep them ON in RPMI+FBS10%, cold PBS (4ºC), or cold methanol? We perform both...
31 December 2017 7,761 8 View
Blood from some donors are fatty, after centrifugation an upper layer forms which is difficult to remove, in order to get clean plasma for cfDNA analysis. Any advice? Thanks!
01 February 2017 5,871 5 View
Any free software for pie charts for quality images, thank you!
11 December 2016 2,661 10 View
10 November 2015 339 1 View
How can I improve ethanol removal from final suspension?
06 July 2015 8,200 2 View
I want to remove most of ethanol following DNA precipitation, thanks!!!
06 July 2015 4,391 6 View
Data sheet from Novared (Vector) says "Prolonged incubation in alcohol or use of alcohol-based differentiating solutions may decreasesensitivity" and "Do not use recycled alcohol with this product".
31 December 2014 3,341 2 View
I am preparing some figures that require more elaborate schemes & drawings
05 June 2014 4,856 4 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View