How to preserve best PBMC samples for performing FACS staining later ON?

31 December 2017 7,664 8 View

How to remove fat from blood to get clean plasma for cfDNA analysis?

Blood from some donors are fatty, after centrifugation an upper layer forms which is difficult to remove, in order to get clean plasma for cfDNA analysis. Any advice? Thanks!

01 February 2017 5,796 5 View

Can you please recommend me any free software for pie charts?

Any free software for pie charts for quality images, thank you!

11 December 2016 2,572 10 View

Christmas gift!!! Can you recommend me any free software for pie charts?

Any free software for pie charts for quality images, thank you!

10 November 2015 256 1 View

How can I improve ethanol removal when precipiating DNA?

I want to remove most of ethanol following DNA precipitation, thanks!!!

06 July 2015 4,322 6 View

How can I improve DNA solubilization when using DNAzol?

How can I improve ethanol removal from final suspension?

06 July 2015 8,128 2 View

When performing immunohistochemistry and revealing with Novared, signal goes away when I dehydrate the slides. Any advice?

Data sheet from Novared (Vector) says "Prolonged incubation in alcohol or use of alcohol-based differentiating solutions may decreasesensitivity" and "Do not use recycled alcohol with this product".

31 December 2014 3,270 2 View

Can anyone recommend me an easy software for drawing?

I am preparing some figures that require more elaborate schemes & drawings

05 June 2014 4,780 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

No title

02 March 2021 3,060 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View