Contact experts in Nucleic Acids to get answers
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Questions related to Nucleic Acids
Hello, can i ask a question that can any one explain me the difference between nucleic acids and amino acids, in the sense of which one is lighter or heavier,? next is then which one is easier to...
06 June 2016 518 0 View
I'm planning to investigate cell free circulating nucleic acids in phloem sap in higher plant species under different physiological states and\or stressful conditions. Sure, Arabidopsis thaliana...
05 May 2016 5,161 6 View
ligands (picture) Kq(103 M-1) 11,27,418,46,911,3 thank you
05 May 2016 3,595 0 View
HIV structure is composed of two copies of positive single stranded RNA that codes for the virus's nine genes enclosed by a conical capsid composed of 2,000 copies of the viral protein.
04 April 2016 8,339 0 View
Ok, it is clear for us the Gustavo Acevedo-Hernández´s answer, but Reading some publicactions many people has doubts about the three concepts. Ex: "Modules can be understood as a separated...
04 April 2016 2,083 2 View
Is there any reference article for this? Cts < 29 are strong positive reactions indicative of abundant target nucleic acid in the sample Cts of 30-37 are positive reactions indicative of...
23 March 2016 3,172 3 View
Can someone tell me if epperndorf biospectrophotometer with micro cuvette is really better than Nanodrop in nucleic acids quantification, even for small concentrations?
03 March 2016 1,732 6 View
How can I concentrate extracted nuclic acid after extraction?
03 March 2016 3,290 6 View
I'm looking for a polymer that is selectively biodegradable in a cells nucleus and is not toxic for the cell. Also, it should be able to encapsulate nucleic acids without degrading them.
03 March 2016 8,266 0 View
Currently, we are really confused by a new function of Bst DNA ploymerase that has been commonly used for isothermal nucleic acid amplification. The phenomenon caused by the funtion seems like the...
02 February 2016 6,569 1 View
Polycations are necessary to escort nucleic acid in order to be uptake into the cells. How that polyplex could escape from the endosome?
02 February 2016 3,123 1 View
I need to determine if a gel running area has been successfully decontaminated from EtBr and another area successfully decontaminated SybrSafe. I have checked both areas with a handheld lamp and I...
02 February 2016 2,398 3 View
1. How to digest plasmid completely, without even one copy of intact circular plasmid? 2. Is it possible to insert this DNA fragment into the genome of TG1 cells randomly? It looks possible from...
02 February 2016 8,468 3 View
Hello, everyone. I am working with HEK 293 cells. I have been transiently transfecting them with 8mg DNA in 500micL serum free media. I use 1:10 DNA/PEI ratio, such that I have been adding...
30 January 2016 5,546 3 View
I would like to quantify the total amount of nucleic acids in a sample, including human nucleic acids and bacterial nucleic acids. I don't care whether they are in double-strand, single-strand, or...
01 January 2016 8,602 3 View
hello everyone, I am using RexMD method for molecular simulations of a nucleic acid, using AMBER 14. I am following the AMBER TUTORIAL page where i have built the structure and started the...
01 January 2016 2,774 0 View
01 January 2016 9,084 4 View
I am doing a Megascript RNA precipitation reaction using Sp6 polymerase. After the reaction, I add 1uL DNase for 15mins, then 1uL EDTA to quench. After adjusting volume to 50uL, I add 5uL 5M...
12 December 2015 1,577 2 View
as the combination of these agents ( chaotropic and non-chaotropic )can give different features then using each one of theme alone, so what results and purity, what kind of nucleic acids can be...
12 December 2015 1,687 0 View
Dear experts, I've got some problems during extracting urine nucleic acid using carrier RNA to get more products. But the results showed that in the lasts products there are much more carrier RNAs...
12 November 2015 9,530 1 View
11 November 2015 3,401 1 View
How can treatment of DNA samples to remove contamination protein? Can i use PCR in tissue samples if protein found about 260/280 0.77 and concentration of nucleic acid about 75 ng/ml?
11 November 2015 8,293 7 View
absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm, does it mean the protein solution contain dimers/oligomers or does it contain nucleic acid contamination?
29 October 2015 383 2 View
10 October 2015 10,000 3 View