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Questions related to Nucleic Acids
I know that DNA adsorption to plastic container surfaces (like polypropylene) can result in loss of sample, but I wanted to know specifically how much. I know this depends on several factors, like...
08 August 2019 4,069 2 View
I tried extracting DNA from Garcinia fruit and got the following readings with Nanodrop. Nucleic acid: 149.7 ng/µL A260/A280: 1.39 A260/A230: 0.12 A260: 2.99 A280: 2.15 Factor: 50 Baseline...
31 July 2019 5,621 9 View
I am developing nucleic acid based lateral flow immunoassay. I am getting false positive while running buffer (borate, pH 8.5, 1 % BSA, 0.05 % Tween 20) only. My system consists of gold...
30 July 2019 5,020 4 View
How to get the parameter file for GTP or GMP containing nucleic acid in AMBER or NAMD?
21 July 2019 9,646 3 View
My guess is that 20% is strong enough to effectively kill cells and denature proteins + nucleic acids, and that any higher concentration would be overkill. Also 20% is relatively safer for the...
15 July 2019 5,301 2 View
07 July 2019 9,721 2 View
07 July 2019 1,546 10 View
07 July 2019 8,532 4 View
Hello to the scientific community in Research Gate, I got unexpected results during my latest DNA and RNA extraction . I had to perform a DNA precipitation step adding isopropanol and sodium...
06 June 2019 9,233 3 View
I am working with disassociated cells taken from frozen tissue (-20 C) and need to stain their nucleus, but it is not working. I am unable to preserve fresh tissue in PFA or GLA before freezing it...
06 June 2019 9,418 2 View
Good afternoon, Propidium iodide (PI) is a dye use to stain nucleic acid in flow cytometric analyses, particularly in cell cycle analyses. Has anyone dared to try staining a DNA electrophoresis...
30 April 2019 7,331 3 View
Without flow of information from protein to nucleic acid (opposite to the central dogma of life), it is really difficult to describe how our immune system function and long term memory form. This...
07 April 2019 4,236 3 View
I want to infer the ambiguous nucleic acid of genome. I have these data set below. ambiguous data 1 2 3 4 ・・・・・・100 A AorT A T ・・・・・・T A GorT A G ・・・・・・T ・ ・ ・ complete...
04 April 2019 6,163 3 View
04 April 2019 4,611 5 View
04 April 2019 4,103 3 View
150 years ago (more precisely, 150 - 149 years ago) Johann Friedrich Miescher working in Felix Hoppe-Seyler's laboratory at the University of Tübingen discovered DNA (nucleic acids). Around the...
03 March 2019 4,195 1 View
Very hard to avoid over-drying or under-drying just by visual inspection. Also highly subjective and inconsistent between my lab team members.
03 March 2019 7,431 6 View
DNA purification
02 February 2019 6,446 1 View
Dear all, I would like to know if any of you have experience in dephosphorylation of proteins using Shrimp Alkaline Phosphatase. I am currently buying lambda phosphatase, but since I had SAP in...
02 February 2019 8,062 1 View
I am looking for a nucleobase (utmost its derivative) which shows reversible redox activity. I went through some of the literature and found out that the redox features of nucleobases are very...
21 January 2019 3,382 3 View
My lab has employed a FRET-based nucleic acid cleavage assay with a fluorescein labelled RNA bound to a DNA with black hole quencher. Historically, we would always get picture-perfect hyperbolic...
12 December 2018 9,026 2 View
Separation of Bio-molecules by affinity chromatography Most specifically i am in search of DNA and RNA purification by using affinity chromatography.... As DNA affinity chromatography and RNA...
12 December 2018 3,719 4 View
I am wanting to analyse soluble components in a plant tissue extracted into TCA. I would like to be sure that I have extracted all that I can whilst removing high molecular weight materials such...
03 December 2018 6,943 1 View
I noticed we've been using a PCR system in our one-step RT-PCRs that incorporates dUTPs and uracil-DNA glycosylase (UDG). The UDG is not the cod-derived version. Our reverse transcription (RT'n)...
11 November 2018 9,082 0 View