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Questions related to Nucleic Acids
Looking for a way to preserve nucleic acids on DBS. Currently using 3MM filter paper which is great for storing blood samples but we need a way to ensure quality long term.
06 June 2018 610 0 View
Dear All, I am expressing a GST fusion protein in Bacterial cells and on purification of the protein, there are a lot of contaminating nucleic acids. I tried various approaches to get rid off the...
06 June 2018 6,197 5 View
I have 20 fresh frozen brain samples from different patients and I need to cut them using the cryostat (at -20 C), so that 10 slices will go on glass slides for IHC and 15 will go on three tubes...
01 June 2018 3,428 2 View
Hello colleagues, Nucleic acid purification from plants, animals and microbes ... I wonder if this method works in other people's hands, particularly for adherent mammalian cells. I also wonder...
05 May 2018 3,960 0 View
I am working on Gold nanoparticle based Nucleic acid lateral flow assay. I want to know the sensitivity of each label digoxigenin (dig), carboxyfluorescein (FAM), FITC, streptavidin and biotin....
17 April 2018 8,824 2 View
I am trying to label my RNA with cystamine on it's 3' end and I have done periodate oxidation to get an aldehyde from the diols. But the cystamine labeling did not work. I followed the exact...
13 April 2018 9,497 3 View
Hello everyone! this is quite common/rare observation that auto-activation of some genes apart from transcription factors are really a fascinating during Yeast two hybrid assay.But how can we...
04 April 2018 5,555 0 View
04 April 2018 2,158 2 View
DNA quantity (Concentration) is very important step in pre-PCR technique.
04 March 2018 6,116 20 View
I am carrying out molecular dynamics simulation of drug-dna system; after having had removed the nucleic acid from the complex when i proceed to to the MD via AMBER force field in UBUNTU system i...
03 March 2018 1,177 2 View
I'm making nucleic acid extraction with Maxwell RSC equipment. But the purity 260/280 is not too high. I extract the nucleic acids from plasma samples. So I'd like to know if there's anyway to...
03 March 2018 3,134 4 View
We are going to buy a LED transilluminator for our laboratory, but we usually use EtBr, I want to know how accurate these transilluminators are? I mean, can these transilluminators show the...
07 February 2018 1,340 8 View
The aim is to preserve the most of RNA structures/base pairing after extraction of RNA from virus. so the question can really be read as: regarding the common RNA extraction methods, what are...
02 February 2018 7,334 2 View
Is nucleases related to calcium ion? Is there any chemicals that can show the same function ?
02 February 2018 5,858 0 View
Hi all, I looking for literature evidence for this phenomenon. Apparently, RNA (I'm not sure if also DNA) is cleavaged in a non-enzymatic manner by iron. I would need some theory to support...
20 January 2018 3,275 4 View
One of my juniors wants to detect LAMP products by using SYBR Green I but he failed to find any protocol for staining nucleic acid directly. Can anyone please help in this regard?
20 January 2018 5,788 4 View
I'm trying to solve an assignment question where we are given a Amino Acid sequence and was asked to use this a basis to design probes and select clones? The aminoacid sequence is...
01 January 2018 3,549 4 View
I believe that some forms of radiation can denature proteins. But can any sort of electromagnetic radiation actually cause changes in nucleic acid composition? Specifically, can portions of bases...
12 December 2017 6,744 6 View
I have found that the A260/A280 was apparently first introduced by Warburg and Christian in 1942, but I haven’t been able to find the original publication about the A260/A230 ratio. Can anyone help?
12 December 2017 3,448 3 View
Hello Everyone, I face trouble when I align forward and reverse sequences in BioEdit. When I give the command of Pairwise alignment, software creates the alignment, but in aligned sequence, some...
12 December 2017 5,222 5 View
07 December 2017 7,019 5 View
I am intended to sequence (via Illumina) viral nucleic acids, including RNA. I was informed that using RNA carrier is not recommended in this case. However, since my samples will have a low...
30 November 2017 8,507 2 View
11 November 2017 4,762 2 View
I'm looking to the best method to extract both RNA and DNA from the same sample (mice brain tissues). Sometimes we just separate the tissue in the 2 hemisphere, but this is not the case. I tryed...
25 October 2017 959 3 View