Using a thermal melting study, is it possible to evaluate thermodynamic parameters easily?
… not clear what “CD melting study” means. Do you want to melt a sample while it is inside a CD spectrometer? Is it a pure substance or a mixture?
Thank you for the clarification! I am not a biochemist, so I am not sure that I can give you good advice in this matter. However, to me this looks like a case not of “melting”, but a chemical reaction. If the circular dichroism changes during the reaction, you should be able to follow the reaction. If the separation of the DNA strands is a reversible chemical reaction with no kinetic hindrance, you should be able to obtain something like a reaction enthalpy; if the reaction is significantly controlled by kinetics, you would extract the activation energy.
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