Other than the usual method of adding divalent cations of small ionic radii, is there any better method? I want very clean background.
I wonder how can a Physicist work with live animals as a PI without collaborating with biologists.
06 July 2018 7,924 8 View
The electrodes are used for voltage measurement in the Brain and needs to be connected to pre-amplifiers. Is there any company or research group that can fabricate transparent microchip for this...
09 October 2017 2,105 8 View
I have different samples with height in the range of about 1.5 to 3 nm; as measured by ScanAsyst mode in air, with same AFM probe and same imaging force. I know that height measured by tapping...
31 December 2016 2,190 5 View
I have colored the molecule with b-factor and I don't want any lighting or shadows to influence this coloring.
03 April 2016 1,252 3 View
I am trying to estimate the width of DNA from its crystal structure.
08 September 2014 9,897 6 View
For force spectroscopy experiments with AFM, it is needed to calibrate the deflection sensitivity and spring constant of the cantilever. First I determine the deflection sensitivity very...
04 May 2014 4,770 17 View
I am imaging DNA using AFM. I extracted the gray scale intensity values from the image into a matrix of integer elements. Then I wrote a code in C++ to count pixels falling within a certain...
02 March 2014 5,079 28 View
Are the isolation tables using nitrogen or compressed air supply the best? What do you think about the active isolation platforms available from Herzan and Accurion that runs simply on electricity?
09 October 2013 4,461 13 View
Two special cases: 1. Both the promoter & terminator sequence is present. 2. Only the promoter sequence is present.
01 January 1970 6,670 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View