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Questions related to Next Generation Sequencing
I am looking for opinions/recommendations of NGS Platforms (Illumina vs. BGI/MGI) from people that had opportunity to work on both hardware: MGISEQ-T7 and NovaSeq 6000 or analyze data from both....
05 October 2020 8,201 1 View
Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...
29 September 2020 2,467 1 View
Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.
28 September 2020 966 2 View
I am analyzing a fish gut (16S rRNA gene) microbiome dataset. I have some continuous variables (e.g. weight, blood cortisol levels) and want to see if there is any correlation between bacterial...
03 September 2020 7,026 3 View
Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...
26 August 2020 8,615 1 View
I ordered 16S primers according to the earth microbiome website, however, the researchers added GCT to the end of the Forward primer Illumina 5' Adapter to affect the melting temperature of the...
14 August 2020 1,784 1 View
Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...
12 August 2020 525 3 View
I wanted to learn R and Python for genomics work and i have experience in using GUI platforms like Galaxy and CLC for NGS analysis. I am now looking to expand my knowledge in coding based NGS...
01 August 2020 6,765 9 View
Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance
28 July 2020 9,923 5 View
what companies can provide Next generation sequencing services for free or at the lowest cost?
26 July 2020 6,644 3 View
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
22 July 2020 1,961 4 View
Hi Dear colleagues I've got a FASTQ file that is produced using Thermofisher Ion S5 machine, I want to analyze this raw data using NIPTMer software: Main article: NIPTmer: Rapid k-mer-based...
28 June 2020 2,942 0 View
Hi We've got an Ion S5 System (Thermofisher NGS System) at our lab, but suddenly we lost the SSD's (for electrical problems) and now we need to re-install the ubutnu (server and client) and all...
10 June 2020 2,732 1 View
Is there any treatments for this disease? A 11 months old child was just diagnosed with Schindler disease type 1. The parents are really concerned. There are only 20 cases in the world with this...
02 June 2020 609 0 View
I want to sequence the amplicons with two different library prep kits. 1. Nextera DNA Flex Library Prep. kit 2. QIAseq Ultralow Input Library Kit Can I use the libraries from both preparation...
01 June 2020 1,826 3 View
I am looking for explanation in the context of NGS variant calling pipeline
28 May 2020 1,058 1 View
We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...
25 May 2020 8,162 3 View
Greetings! I am dealing with NGS data. I have a vcf file of the called SNPs. I want to do population diversity and differentiation analysis with it but unfortunately, I unable to do so though I...
13 May 2020 5,859 3 View
My group would like to perform sequencing through Next-Generation Sequencing (NGS) in SARS-COV-2 isolates. However, although many groups have used this technique recently in their research, the...
29 April 2020 4,555 3 View
Yesterday I published "GCBS Bibliographies: Sources and Applications" on ResearchGate. However, I failed to request assistance in expanding the bibliography of applications. If you know of other...
16 April 2020 5,088 0 View
I saw this cross in an NGS video and photographed the screen, but I did not learn where this cross was found. Seems to me that it was found in some island. In Cuba there is one very much similar...
26 March 2020 2,377 2 View
I have been using a set of PaxGene tubes to collect blood samples from which I have been isolating cfDNA for NGS analysis. I need to collect more blood samples, but I noticed that the expiration...
10 March 2020 4,084 1 View
NGS data and I want to have multiple consensci based on those reads.
04 March 2020 6,504 1 View
what is the longest deletion or insertion that can be detected in next generation sequencing data analysis
27 February 2020 2,937 3 View