Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Only one clone of purified protein is active?

Hi! I'm wondering if anyone else has had a situation where they are purifying an enzyme, and only one clone is active? The cloned gene is genetically identical to other clones, but only this one...

18 September 2020 7,984 3 View

How can an enzyme have multiple optimal pH values?

Hi! I've been studying a particular enzyme that in in vitro experiments exhibits two optimal pH's. All other parameters are the same (incubation time, substrate, temperature, salt concentration,...

16 August 2020 6,073 10 View

Best phylogenetic analysis for a small data set?

I have a small data set consisting of 16 sequences, 919bp each. I am trying to determine the phylogenetic relationship among the individuals. I am wondering if the neighbor-joining method, maximum...

15 July 2020 3,240 8 View

(Geneious software) How do you DELETE the 'bad nucleotide' symbol of "?" from genome sequence?

Hi everyone! (I'm hoping you're all safe and healthy) I could really use some help figuring out how to remove the bad nucleotide symbol "???" in my Geneious sequence. (See attached photo) Before...

18 May 2020 7,840 1 View

What are the spectroscopic technics used in biological chemistry or biology ?

I want to kno more about the technicqs people usually use to learn how it works.

21 February 2020 6,917 2 View

How do I interpret interactions with the covariate in a repeated measures ANOVA?

I am running an analysis on repeated measures perceptual data (psychology). There are three categorical factors which specify the conditions participants viewed the stimuli in (e.g mode of...

30 January 2020 2,209 3 View

Can I perform XRD analysis on polished sample?

Dear All, I have to analyze a Co based superally sample where are present two different crystal structures of Co (the cubic and the exagonal one). I'm sure of it because I have analyzed the...

14 November 2018 3,667 3 View

Is there a product that allows mammalian cell lines to be stored at ambient temperature (18-25oC) for 96 h?

Hi I'm looking for a reagent that can be used to maintain cell viability at room temperature for at least 96 h. Does anyone have a recommendation? Thanks.

02 August 2018 8,793 4 View

SPSS:What should I do when my -2 log likelihood is really high, r squared value really low and a 0.000 result for the Hosmer-Lemeshow goodness-of-fit?

I am currently doing my masters thesis which involves data analysis using SPSS, the dataset has over 135000 items and numerous variables. I performed a test of multicollinearity for my...

22 July 2018 2,214 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View