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Questions related to Molecular Biological Techniques
In what ways is conservation agriculture farming different from conventional farming?
03 June 2024 227 6 View
How do you indicate soil fertility and which soil characteristic is an indicator of fertility?
08 May 2024 8,136 9 View
I attempted to tag my RFP to Gram-positive bacteria, but upon checking, the final color appeared green instead of the expected red. I'm seeking guidance to understand what might have gone wrong in...
26 April 2024 7,418 3 View
Based on the article, the analytical performance of DI-60 has been evaluated in line with its capability to recognize various RBC morphology and WBC differentials accurately. I am eager to know if...
11 April 2024 9,498 1 View
Can algae be used as a sustainable alternative to fuels if so, how effective is it compared to other biofuels?
26 March 2024 4,146 3 View
Chlorophyll pigments were extracted according to the Arnon method using 80% acetone. Can anyone suggest me if the same extraction can be used for any other physiological experiments like other...
28 February 2024 3,151 3 View
I have PAR data in W/m^2 but don't know how to convert to molquanta.m^-2.d^-1
18 February 2024 1,978 2 View
I'd like to do a test of human blood hemolysis and I heard that people donate a drop of their own blood to do the test. Is there anyone who can tell me how to do this?
01 February 2024 3,448 2 View
How heat is transferred from one object to another how temperature and pressure can impact the the kinetic energy of particles in a system?
25 January 2024 9,580 5 View
Hi! Does anybody use StepOnePlus™ Real-Time PCR System software on Macbook? I know there is a Windows software which I also use in my lab on the computer that connected to the instrument but I...
11 January 2024 1,821 0 View
We had to put our pJet1.2 plasmid in a XL1-Blue Competent Cells. The result were very small colonies on a LB agar medium with ampicilin and IPTG. bands with a very low concentration were visible...
15 December 2023 378 3 View
what are the benefits of a zero carbon fuel emmissions to the solar company
11 December 2023 6,557 4 View
Hello everyone, I was wondering how long before exosome/EV isolation should I withdraw human platelet lysate (serum) from cell culture media (considering mesenchymal stem cells)? I know that the...
06 November 2023 5,972 2 View
I am using 12% gel. Tissue was lysated using RIPA buffer and doing semidry transfer.
20 October 2023 6,062 2 View
Hi everyone, I am working on microalgae I am finding difficulty in maintaining the cultures. After 20 days microalgal cultures are turning brown. I used antibiotics, garlic extracts but not...
11 October 2023 5,098 5 View
I am looking for suitable protocols.
28 September 2023 2,235 1 View
How heat is transferred inside the Earth through convection currents and type of energy transfer is involved in the ground heating the air above?
24 September 2023 8,055 3 View
Exploring the feasibility and effectiveness of using digital platforms to disseminate agricultural knowledge and resources to remote areas in Bangladesh.
24 September 2023 3,650 1 View
Hi! I will be performing transformation in E.coli Stbl3 strain followed by plasmid extraction. However, the Stbl3 strain is EndA+ strain of E. coli . Can someone please guide me with some...
13 September 2023 8,557 3 View
I am trying to choose a strain for expression of a plasmid. Which one would be better?
07 September 2023 6,621 1 View
After overnight (12-16 hrs) incubation of E. coli in LB, when I attempted to extract DNA using Kit, it yielded a low concentration of DNA (10-20 ng/µL). Can anyone help me out to improve its...
28 August 2023 6,845 5 View
Hi everyone, I am doing auditory brainstem response measurements using powerlab AD instruments, but I am not getting the responses for lowest dB level as they are too weak to be measured by...
14 August 2023 3,242 0 View
I am in isolation process of zymomonas mobilis bacteria. So i need to know that there is any media for selectively isolate the zymomonas sps.
10 June 2023 3,892 0 View
Feel free to help me figuring out.
29 May 2023 4,686 0 View