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Questions related to Next Generation Sequencing
What might be a cause for this sideway tail? Is my wells leaking? Or is it because I didn’t add enough running buffer?
27 June 2025 9,906 1 View
Hi dear friends; I have encountered a very serious challenge these days about primer designing for mature miRna gene and I'm seeking help from you right now. I have designed a stem loop primer and...
23 May 2025 9,957 5 View
Hi everyone, I recently ran a test with our new, shorter primers for DNA barcoding, but the results have been unexpected. Below is a summary of the experimental setup and observations: Primers...
22 March 2025 2,140 1 View
Hello everyone, I am having an issue with my LAMP reaction for target DNA detection (30ng). I was using the standard NEB protocol using BST 2.0 polymerase, with 1.6 uM FIP/BIP, 0.2 uM F3/B3 and...
07 February 2025 2,243 4 View
can I get an API for researchGate? is there any?
09 January 2025 8,918 2 View
Hello, I'm a Msc student who is working on some Drosophila Melanogaster Bicoid Homedomain mutant lines, I did molecular staining on embryos which is tagged for Bicoid and obtained Confocal images...
03 January 2025 6,163 0 View
Difficulty in Standardizing Long-Range PCR for CYP17A1 Gene in Congenital Adrenal Hyperplasia (CAH) Hello everyone, I am an intern at the Centre for Cellular and Molecular Biology (CCMB) working...
31 December 2024 8,000 4 View
I am using HDOCK to perform molecular docking, but HDOCK has a limit of 500 nucleotides for the receptor. However, we want to perform docking on the entire 3' UTR region. Can anyone suggest a...
16 December 2024 7,627 4 View
Hello dear academics/researchers I want to use a test for robustness after conducting KRLS test. When i was looking for alternative tests i found lpoly and localp (advanced version of lpoly)....
12 December 2024 8,446 1 View
I recently received a comment from one of the reviewers regarding the use of modification indices (MI) so as to improve my model fit. It says that this approach generally lacks interpretability...
09 December 2024 3,586 2 View
Hello! I am a Berkeley undergrad conducting a research project on love at first sight with a group of other cognitive science/psychology students. The survey below takes about 2 minutes, and I'd...
28 November 2024 1,243 2 View
Hello, I am currently seeking a research question for my systematic review in the field of physiotherapy. The search terms must yield no more than ten relevant studies and a minimum of five that...
22 November 2024 4,967 6 View
Hi, I'm a master's student in biotechnology and I'm having trouble visualizing plasmid bands during extraction. I'm working with the plasmid pET-32a in *E. coli* DH5α. I've tried both kit-based...
20 November 2024 6,990 3 View
Hello everyone. I`m getting a not quite good amplification in the LAMP reaction (see pic.1), but I have received change in color of pH-sensitive dyes in both variants (pic.2). 2x buffer (2,8mM...
25 October 2024 3,628 0 View
Background: I conducted PCR on several grids plate isolations to amplify the DNA for gel electrophoresis. Before running the gel I noticed sediment, or precipitant in my PCR tubes. I thought that...
25 October 2024 7,878 1 View
I am a senior medical student in Zagazig University faculty of medicine (Egypt) with a strong interest in neurology, cardiology, and hematology/oncology, and I am eager to expand my research...
19 October 2024 7,969 4 View
I've been working with a lymphoma cell line (Eµ-Myc) for a few years now. I injected a million cells intravenously into recipient mice and usually cells home into peripheral lymph nodes and...
10 October 2024 924 0 View
I am using PCR product as my template for in vitro transcription and then loading the Rna samples on 8M urea gel. I am observing multiple bands of both low and high molecular weight(my intended...
27 September 2024 6,183 6 View
I am conducting a 2% agarose gel electrophoresis to analyze polymeric nanoparticle encapsulation of Cas9 RNP. My sample set has a charge range from 0 to +10 mV, and I am observing that the more...
24 September 2024 4,590 1 View
For the procedure, I prepared the LAMP reagent mix with 1x isothermal buffer, 6 mM MgSO4, 1.4 mM dNTPs, and 9 µL nuclease-free water. I then added a primer mix consisting of FIP and BIP at 1.6 µM...
03 September 2024 7,221 1 View
I have a plasmid (~9.3kb) with a small 20 nt insert, which I created using the Q5 Site-Directed Mutagenesis Kit via PCR. Before transformation, I obtained a clear band at 9.3 kb. I picked up a...
14 August 2024 5,543 3 View
Actually I have collected some cell line from literature review like CaCo2, HUVEC, RAECs, HEK293. So can anyone suggest me more cell line for Angiotensin Converting Enzyme and Carbonic Anhydrase 1...
19 July 2024 3,726 1 View
With a stressful lifestyle, we start experiencing forgetfulness at an early age, which can accelerate the onset of Alzheimer's disease symptoms in young people as early as their 30 years old....
25 June 2024 993 1 View
When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...
20 June 2024 7,180 5 View