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Questions related to Next Generation Sequencing
on the basis of memories, identities, power relations, and the city's genealogy
08 June 2023 2,194 0 View
Can any one kindly let me know the normal, mild, moderate, and severe levels of lead and arsenic in Urine ?
20 May 2023 5,700 0 View
I stained one cryovial PBMCs according to the CFSE protocol (invitrogen C34570). One day before staining, I thawed the cells and rested them in media (ingredients is below ) for 24 hours. On the...
02 March 2023 4,612 2 View
Does anyone have experience with using Amaxa nuclefactor or Neon for electroporating CRISPR plasmids or any other vectors in primery cells?
01 March 2023 6,778 2 View
I have the whole transcriptome of a non - model fish. I need to identify transcribed pseudogenes from it and do functional analysis of the pseudogenes. How to make the technical programme for this...
25 February 2023 1,148 0 View
I have isolated DNA from FFPE tissue of HCC. The DNA conc. i get is 29.6ng/ul using nanodrop How much DNA should be required to run NGS ? Can anyone pls help or let me know any other...
23 February 2023 6,327 2 View
I need aluminum oxide with a size of 3.8 µm for wear resistance applications. Can someone suggests me a supplier in europe please ?
07 February 2023 9,001 1 View
I have recently been attempting to isolate RNA from polymer scaffolds using the Dynabeads mRNA DIRECT Purification Kit. When I measure using a nanodrop like I would for other extraction methods...
03 January 2023 2,431 3 View
Hello, I am researching the phylogenetic tree of snakes. (bayesian Inference tree) My sequences are 1300 bp, but sequences of outgroups are about 600 bp. Can I change the difference of about 700bp...
12 December 2022 9,251 1 View
Hello! I am currently conducting experiments in which I am staining myofibroblasts and myotubes. To distinguish cell types I would prefer to have a multicolour staining comprising of 4...
29 November 2022 9,107 2 View
Hi, I'm interested in learning QSAR and docking but I don't know which software is versatile, and have a good support and community (preferably free). Any good recommendation? I'm working on...
14 November 2022 4,909 3 View
Hello I am trying to find out the exact binding sites of a number of peptides against a specific protein target. I did docking using many different servers and obtained many divergent results. How...
13 November 2022 3,047 3 View
I've started doing sanger sequencing recently and only rarely get good results. Every run I do barely has >100 Q20 and always has weak signal and excessive background noise. I've attached an...
11 November 2022 2,323 2 View
Hi, I 'm new to MD simulation. I was performing replica exchange method from Amber tutorial. In the tutorial developers uses Amber16 and they get the remd.mdcrd* files in human readable format...
09 November 2022 4,549 1 View
Hi everyone, I am looking to get some human insulin for cell culture work I have planned - I've ordered from Sigma but it won't get here till Christmas and I would rather get started sooner if...
02 November 2022 8,738 1 View
i would like to know what is the meaning of transcriptomic analysis, how it can help in studying the mechanism of action of drugs against resistant microorganisms
23 October 2022 2,266 2 View
Hello I have to enlarge divergence point in phylogenetic tree. because It is difficult to identify the node because there are few mutations and it is too close. (Black dots are nodes)
22 October 2022 5,013 1 View
All the information I know so far is, A relaxed molecular clock is used, the mutation rate is 1.5% per mya, and normal distribution prior and GTR+I+gamma model. how do I setting priors and rate...
17 October 2022 9,058 0 View
Hello everyone. I am trying to do a molecular dynamics simulation on a docked complex of a protein receptor and a peptide ligand. However, I have encountered a major problem in the pressure...
11 October 2022 5,256 0 View
I am beginner in western bloting in our lab there is no gel doc machine so can I keep my membrane in PBST at 4 degree for a week and take image after week going to another lab will it work ?
09 October 2022 2,776 3 View
I am constructing a phylogenetic tree using align sequences of 4 mt-genes [2 coding, 2 noncoding]. Total nucleotide length extends up to about 2500bp. I am stuck in one point, therefore I want to...
23 September 2022 2,077 1 View
The molecular clock will be analyzed using a gene region with little variation between populations. which one I have to use, BEAST or mrbayes? and tell me why. thanks.
16 September 2022 3,415 0 View
We have a GWAS population (genotyped) and we want to select the minimum (an optimal) set of genotype to phenotype in order to detect GWAS signals. Any soft or method available?
09 September 2022 3,598 2 View
if i have the enzyme activity of pectinase (8000-12000 U/g) and cellulase(>= 10 U/mg solid). then how to calculate that particular activity on extraction of lycopene from tomato
01 September 2022 3,461 2 View