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Questions related to Next Generation Sequencing
I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...
17 February 2021 7,669 2 View
We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...
16 February 2021 5,593 4 View
I would like to barcode fungal caps from the field for two main research goals (1) molecular iD of the fungal specimen and (2) to identify any invertebrate DNA within the cap using CO1...
08 February 2021 4,542 1 View
I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.
02 February 2021 8,430 3 View
I have some sequences of Spike protein of some Covid 19 samples that have been sequenced in my laboratory using NGS. For analyses of those, I need to convert those sequences into PDB format. I am...
01 December 2020 739 3 View
Hi there, I am working on airborne microbes. I want to know the concentration of bacteria in my sample before I go for NGS. Since it is an environmental sample it might have a lot of mixed...
17 November 2020 401 3 View
I have about 30 blood samples from people with inherited fructose intolerance who need to use the next generation sequencing technique. Is there a research center or person doing this test to work...
02 November 2020 3,533 3 View
I am doing an analysis with transcriptome data and comparing the same with the Genomic data. More precisely, I am looking at mRNA results of a tumor sample without having the normal tumor samples...
26 October 2020 3,873 3 View
Dear all. We need to sample insects, store them upon collection and ship them internationally. Sampled will later be processed for DNA extraction and NGS sequencing. Keeping all this in mind, can...
25 October 2020 7,462 2 View
I am looking for opinions/recommendations of NGS Platforms (Illumina vs. BGI/MGI) from people that had opportunity to work on both hardware: MGISEQ-T7 and NovaSeq 6000 or analyze data from both....
04 October 2020 8,167 1 View
Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...
28 September 2020 2,389 1 View
Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.
27 September 2020 895 2 View
Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...
25 August 2020 8,539 1 View
I ordered 16S primers according to the earth microbiome website, however, the researchers added GCT to the end of the Forward primer Illumina 5' Adapter to affect the melting temperature of the...
13 August 2020 1,754 1 View
Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...
11 August 2020 460 3 View
I wanted to learn R and Python for genomics work and i have experience in using GUI platforms like Galaxy and CLC for NGS analysis. I am now looking to expand my knowledge in coding based NGS...
31 July 2020 6,698 9 View
Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance
27 July 2020 9,877 5 View
what companies can provide Next generation sequencing services for free or at the lowest cost?
25 July 2020 6,584 3 View
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
21 July 2020 1,895 4 View
Hi We've got an Ion S5 System (Thermofisher NGS System) at our lab, but suddenly we lost the SSD's (for electrical problems) and now we need to re-install the ubutnu (server and client) and all...
09 June 2020 2,674 1 View
I want to sequence the amplicons with two different library prep kits. 1. Nextera DNA Flex Library Prep. kit 2. QIAseq Ultralow Input Library Kit Can I use the libraries from both preparation...
31 May 2020 1,790 3 View
I am looking for explanation in the context of NGS variant calling pipeline
27 May 2020 1,020 1 View
We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...
24 May 2020 8,103 3 View
Greetings! I am dealing with NGS data. I have a vcf file of the called SNPs. I want to do population diversity and differentiation analysis with it but unfortunately, I unable to do so though I...
12 May 2020 5,798 3 View