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Questions related to Next Generation Sequencing
Hello! I have tried to optimise my IHC protocol, but I still have positive cells in secondary antibody control sample (see picture attached) and now I'm thinking will anything help with that....
19 March 2020 7,089 5 View
I have been using a set of PaxGene tubes to collect blood samples from which I have been isolating cfDNA for NGS analysis. I need to collect more blood samples, but I noticed that the expiration...
10 March 2020 4,289 1 View
hi, i have developed a reading test with 24 items, how can i calculate its construct validity through factor analysis? i mean how can i enter its items into the spss , while each item has a...
06 March 2020 5,103 3 View
NGS data and I want to have multiple consensci based on those reads.
04 March 2020 6,607 1 View
what is the longest deletion or insertion that can be detected in next generation sequencing data analysis
27 February 2020 3,295 3 View
Hi all, I am using IDT capture probe to enrich the target region of tumor samples for sequencing. However, I found Skewed GC bias in NGS data, which resulted in poor capture uniformity and lower...
10 February 2020 3,731 2 View
I've completed a genomic DNA extraction using the ThermoFisher Yeast DNA extraction kit on some Basidiobolus ranarum fungal cells. Of note, before performing the ethanol wash for this protocol the...
10 February 2020 7,262 2 View
Our project objective is to investigate the genetic diversity of Palestinian date palm, cultivars. 6 primers were ordered, these primers were designed specifically for NGS analysis. DNA was...
07 February 2020 1,074 2 View
A NGS panel of truseq was performed, the SNP expected of CSN1S2, Ch 6, Position 87266177 are C/T, but we received A/G. There is an explain about that?
29 January 2020 1,402 2 View
I am planning to use a qualitative approach for it because of its linkage with contextual matters. However, people's perceptions (satisfaction) may be assessed by the closed questionnaire i.e....
18 January 2020 7,674 15 View
Dear all, Currently, I am processing a NGS data using cutadapt to remove 3p and 5p adapter sequences from reads. Normally, as I notice, the percentage of trimmed reads (reads with removed...
10 January 2020 8,501 3 View
Hi All, we have a bioinformatics challenge and we would love any help this community can offer. We have data from a target-enrichment experiment that was supposed to capture certain...
10 January 2020 1,217 3 View
I'm using Ambion's TURBO rDNase and I think I'm seeing degradation of my RNA. Why is that? Lane 1 = untreated RNA extract. 23s/16s intensity ratio = ~1.8 Lane 2 = ~0.5 ug of RNA, 37C for 30 min....
01 January 2020 4,090 2 View
I have localised a protein in Endoplasmic reticulum of Saccharomyces cerevisiae. I have tagged this protein with HA tag. Now i want to label this protein with anti-HA fluorescent antibody for FACS...
23 December 2019 5,945 2 View
I have a problem with adapters dimmers to perform an NGS. I would like to know how the best way to separate my library from the contaminant dimer. My library has approximately 200 bp and the...
22 November 2019 2,642 4 View
Species of the sea cucumbers belonging to the genus Thyone are notorious in eviciscerating their internal organs through the mouth when traumatised thereby losing their tentacles, calcareous ring...
19 November 2019 4,081 0 View
Hi All I start sing the software STAMP, My project is to compare two gut microbiota groups. I need to know what is the significant different between this tow group in family level. could you...
18 November 2019 2,869 2 View
Hello everybody! I need to perform new experiment. I want to stain collagen fibers in porcine tissue by means of mouse primary antibody. Then to visualize it I am going to use anti-mouse goat...
10 November 2019 2,631 2 View
I want to carried out a study of karyology in cuban cactus and I would like to know advice from experienced people in this field
01 November 2019 6,305 3 View
There is a task to identify rapidly current variants of potyviruses in potato and tomato plants. What would yo recommend - NGS or Sanger method to make it faster? If NGS, what method shell we use...
07 October 2019 9,778 2 View
Can factor analysis be used for categoring varaibles based on the factors scores in a scernerio where all the varaibles are important and researchers wants to know the degree of influence on a...
02 October 2019 9,062 5 View
Dear all, I want to use cutadapt when processing NGS data in order to remove 4 nucleotides at both 3-end and 5-end of each read. Could you suggest a command that I can use to achieve this purpose?...
24 September 2019 4,722 2 View
I want to isolate DNA and RNA from feline faecal samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. The isolation will not be...
23 September 2019 3,329 4 View
Total whole genome size is 17 Gb but how much is for total exonic region?
17 September 2019 4,696 5 View