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Questions related to Next Generation Sequencing
GBS
21 July 2022 6,035 0 View
Dear Academician and researchers I am writing to ask for favor. I am stuck in computation of population counts and night lights of each grid cell (30 arc second)? Anyone who can help me in this...
15 July 2022 3,723 0 View
I was wondering if anyone had issues with their MiniAmp. Overall, I'm quite happy with the instrument but I find the lid heats/cools extremely slowly (0.3 degrees/sec) and I cannot find any way to...
08 July 2022 7,512 0 View
I want to know how no template control looks with single probe mix. I am seeing a prominent band just above 50 bases when tried with a single probe mix (targeted to one gene) and multiple faint...
19 April 2022 3,875 0 View
I want to prepare an isotonic solution of calcium lactate with respect to the meat sample. In that scenario, I have seen in papers that I have to take the concentration of Calcium lactate with the...
22 March 2022 3,838 7 View
I want to know the concentration of calcium lactate required to store meat to make the solution isotonic. Please share any links or references
02 February 2022 7,870 3 View
In certain industry, there is a huge amount of Calcium Sulphate produced annually as a subproduct. Is there any idea for how to use them or it will be sent to the landfill???
05 January 2022 4,778 6 View
Dear Colleagues, I would need to know your experience when several concentration of ANG II are applied on HUVECs in vitro, in terms of number of viable cells after incubation. We see about a 30%...
30 November 2021 861 2 View
I have been searching the publications reporting STR markers in the genomic neighborhood of my interest, but I wonder if there might be a more straightforward approach to it. Also within the UCSC...
24 November 2021 8,269 4 View
Hi, I want to do GO and KEGG analysis using DEGs/Gene IDs to do GSEA and ORA by ClusterProfiler pipeline in R. As my organism (Brassica napus) is not a model plant, so I cant use R supported...
16 November 2021 1,397 1 View
Hello, I am currently preparing cDNA Library with Nebnext Ultra II Directional RNA Library Prep Kit from exosomal RNA (patients with multiple myeloma). My research is centered around lncRNA. After...
31 October 2021 1,346 0 View
I am testing out 4 different genes (coding for completely different proteins). When I ran an RT-qPCR assay with these genes (all on the same plate, on the same day), I noticed that a couple of the...
31 August 2021 9,869 4 View
In our laboratory, different results were obtained in the MLPA test performed for a gametic mutation on the peripheral blood samples belonging to the same patient with an interval of one year. We...
18 August 2021 506 3 View
I analyzed the neuroblastoma gene on the R2 (https://r2.amc.nl/), and i did Kaplan- Meier. That time KOCAK says the high is worse, and SEQC says low is worse, but is there a case like this? Can...
02 August 2021 6,777 1 View
I'm about to analyze the intracellular Ca+ concentration in HT29 and SW480 cell lines using flow cytometry. I am thinking of using Fluo-4 AM (invitrogen) but I am not sure which would be a good...
14 July 2021 1,438 2 View
Osmoregulatory responses mitigate the passive adjustments in cell structure and the growth inhibition that may ensue. In fungi, the HOG pathway through MAPK is linked to both, osmosensing and...
02 July 2021 9,309 2 View
Hello to you all, I want to perform DNA seq and I am having issues in the step of ligating the adaptors to the sequences. To give you more details: We want to find the different virus detected...
01 July 2021 5,712 0 View
I am having an assembled fasta file (~3.7 GB in size). I tried using the web based MISA v2.1, but it accepts fasta upto 2Mb only. Is there any other alternate software which can be used for a...
13 June 2021 5,826 5 View
Also can anyone tell me, how to interpret incase germline mutations in oncogene like ALK OR MET genes were found??
26 April 2021 891 0 View
If any body used this for splice site mutation analysis i want to ask how long fasta sequence we will paste.sequence of whole gene or just flanking sequences of mutation if a mutation at the end...
21 April 2021 1,824 0 View
1. Can the DNA mutations that do not affect the phenotype be used as the markers to develop human genetic maps? 2. Can the human genetic maps be developed based on DNA mutations that affect phenotype?
04 April 2021 4,429 3 View
We normally use an agarose overlay but would like to test avicel. We carried out the plaque assay as normal and infected half of the wells and left the other half as a control. Upon removal by...
05 March 2021 7,383 1 View
I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...
18 February 2021 7,964 2 View
We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...
17 February 2021 5,997 4 View