How can I increase cDNA concentartion obtained from viral RNA?

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Karen A. Darbinyan

Hello, similar topic discussed here.

https://www.researchgate.net/post/How_to_amplify_a_specific_cDNA_of_a_viral_RNA_from_a_Total_RNA_Sample

good luck!

Abhijeet Singh

First you need to think about the reason why you have low concentration. The solution would be based on that.

But without that information, I can only suggest to concentrate your sample by any methods like speed-vac, binding the nucleic acid on column or magnetic beads and elute with less volume of solvent or just evaporation.

Laurence Stuart Dawkins-Hall

Can you say how you currently make to suggest alternatives?