Contact experts in Next Generation Sequencing to get answers
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Questions related to Next Generation Sequencing
I am planning to do NGS on human blood samples and my targets are 3 known mutations. The next step would be whole exome sequencing to look for any new variants. My question is how does NGS help in...
16 April 2019 1,164 5 View
Our Group is focused on the study of genetic disorders by NGS of rare haemolytic anaemias due to Red Blood Cell defects.
11 April 2019 4,326 2 View
After assembling a virome data obtained from illumina miseq, how we can detect viral quasispecies.
28 March 2019 8,192 3 View
I heard that extracted cfDNA using carrier RNA is not suitable for NGS libaray prep. is that right? I want to extracted high concentration cfDNA using carrier RNA but also worried about failed...
27 March 2019 3,853 1 View
Can someone recommend and method/way to calculate a proper number of cells for NGS? E.g. let's say we know that in the experiment we have 1 out of 2000 with a variant, and it is not present in the...
18 March 2019 9,090 2 View
I want to go for whole genome sequencing using next gen Illumina but i have some problems. Firstly, the organism is a mollicute with a small genome and is an obligate plant parasite with low...
18 March 2019 8,329 4 View
What type of result can be predicted by NGS Data anlysis?
11 March 2019 6,198 3 View
Hi everyone, What is meaning of Read Group (RG) in NGS data? as you can heck in header lines of BAM/SAM files, some of them contain a single RG, while another ones contain several read groups....
10 March 2019 6,505 3 View
Dear all, i need help in finding ssr from my rnaseq data. I already download the MISA program but have difficulities in executing it. can anybody teach me how to run it? anybody have the tutorial?...
10 March 2019 3,758 2 View
We are on the path of developing Genotype panels for QC evaluation of molecular diagnostic kits (HIV, HBV, HCV). What would be a better method to approach - Sanger sequencing method or NGS...
03 March 2019 2,720 8 View
Does any body know roughly estimation of the # of molecules for detection in capillary electrophoresis of fluorescently labeled DNA? There must be information in the literature. For Illumina...
28 February 2019 8,254 1 View
I have blood samples stored on FTA cards for around 5 years and it would be great if someone could share his or her experience with such samples for NGS.
26 February 2019 2,849 1 View
Can anyone suggest me how much would a MeDIP - seq (Methylated DNA immunoprecipitation) cost for one sample, for sequencing Plant sample including NGS and Bioinformatics analysis
16 February 2019 7,693 3 View
It is my first time to analyze sequence data of 16s and CO1 for my population genetics studies. I want to know if there is genetic differentiation of samples among locations. I am planning to do...
13 February 2019 4,045 1 View
Hello, I am trying to increase penetration of my LC3B antibody on 30um brain tissue cryosections. I currently use 10% NGS and 0.1% TX-100 for blocking and then 2% NGS and 0.3% TX-100 for primary...
11 February 2019 4,776 1 View
With 10M testers with atDNA and public access via GEDMATCH, dozens of law enforcement agencies in the US are using this source with amazing results for cold cases and unidentified remains. YDNA...
24 January 2019 1,352 5 View
Hi everyone, I would like to use the CADD Score to determine the deleteriousness of novel genetic variants. The web interface requests a VCF file as input file type, unfortunately, I do not know...
23 January 2019 668 0 View
Hello everyone I'm working on the genome processing of a fish species based on Next Generation Sequencing method. There's not been previous reports of this species sequence and now I have also raw...
18 January 2019 3,927 3 View
I injected my rat via intravenous tail vein injection with an AAV2-CBA-GFP virus... waited 14 days for viral infection and then perfused my rat with PBS to wash out the blood and 4% PFA to fix all...
17 January 2019 9,211 4 View
I want to extract DNA from buffy coat (human whole blood). I would like to know that which DNA extraction kit gives the best purity and concentration for NGS analysis? Thank you in advance.
15 January 2019 6,473 7 View
Has anyone had luck with NGS library prep for DNA/RNA samples that had low A260/230 ratio due to guanidine thiocyanate? Our samples are made using the AllPrep Qiagen kit without Trizol (used other...
10 January 2019 6,516 4 View
I am aware that blocking using hydrogen peroxide is needed to prevent endogenous peroxidase activity on paraffin embedded tissue sections to perform immunoflourescence / immunohistochemistry. But...
09 January 2019 4,203 5 View
Hi everyone, I am new to the NGS data analysis. I want to trim my raw ngs data to remove junk adapters using Cutadapt package. Now I have two questions. 1.How can I obtain the sequences of the...
02 January 2019 8,265 7 View
when I constructed library for Next-generation sequencing, the efficience of adapter ligation was low, How can I improve it? chage the enzyme or the condition of reaction.
28 December 2018 7,853 3 View