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Questions related to Next Generation Sequencing
Whoever has an electronic version of this book, sell it to me，《potato handbook crop of the future》Thanks
16 June 2024 4,457 0 View
How can I select desired traits in f2 segregated populations by molecular marker (MAS) in canola (Brassica napus)? (for example markers associated with spring or winter type cultivar, yield-...
15 June 2024 9,585 0 View
The sea horse assay for acini or acinar cells, if anybody is having. could you please share it with me? I have one question regarding the same? Should I do treatment on cells isolated or do...
14 June 2024 9,888 1 View
1. I am trying to install GROMACS 2020.1 on my windows 11 OS, after downloading and extracting the gromacs 2020.1 tar file, it does not have an api folder (however has one api folder in the src...
11 May 2024 6,007 0 View
Hello, I'm quite new here. I'm just following a tutorial based on the folding Trp Cage Amber tutorial. When I try to do the minimization with the following code: pmemd -O -i 1min.in -o 1min.out...
09 May 2024 7,751 2 View
I am doing crosslinking between calcium lactate solution and alginate gel. How do I prepare calcium lactate solution. My approach was 2g of calcium lactate powder and 100g of ultrapure water. Can...
25 March 2024 3,282 0 View
I would like to bring our old BioRad 680 Reader back into service but unfortunately have yet to find the key for the Micoplate Manager Software. We had at one time an Excel file that possessed a...
20 March 2024 9,579 0 View
I was able to locate the minima from a 3D FEL plot in origin. On checking the corresponding PC1 and PC2 values in the file containing time frame vs pc1 vs pc2 data, I could not find the respective...
20 March 2024 2,761 0 View
I used the BIO3D package for PCA analysis and RG(rog), but I did not understand what its output was, how can I save the data other than png format? By the way, I made the simulations using VMD,...
09 January 2024 2,868 1 View
I am trying transfection of SiRNA of COPB2 to pancreatic cell lines in 96 well. Bxpc-3 is difficult to transfect SiRNA following the manufacture's protocol (lipofectamine 3000). So, I have...
27 December 2023 4,105 1 View
I am trying to evaluate the genetic homogeneity of wild grown mother plant and tissue culture raised plants of Piper sp. using SCoT and other dominant markers.
07 December 2023 1,879 0 View
Any procedure or video would be helpful.
27 November 2023 7,497 1 View
I'm looking for anti GFP fluorescent nanobodies conjugated with Alexa 647 (or equivalent) for immunostaining of samples with GFP in order to do dSTORM imaging. Does anyone know of any commercially...
21 November 2023 4,272 0 View
I am sure I have added the sample . So what could be the reason. I understand low quality reads but zero reads. Did anybody faced the same situation? Kindly help. Thanking you in advance.
14 November 2023 1,625 0 View
Hello.. I identified SSRs using the web server MISA and now I want to asses whether they are single copy or multi copy loci. I found that read mapping is an insilico method that can be used for...
26 October 2023 8,984 2 View
The genotyping data we have comes from a GBS analysis, I added screenshot from our data as example. We also have our GBS data available in a VCF file. The phenotypical is scoring data from a Bio...
18 October 2023 9,007 0 View
Do I understand correctly that heterozygous Pmel mice have twice as less cells that carry anti-gp100 TCR? This should be right because of the allelic exclusion. If so, can one combine both homo...
06 September 2023 302 1 View
respected sir/madam i am doing research on clostridum difficle. The problem i am faing is sometimes the bacteria grow sometimes it does not grow but i am performing the same procedure. The...
31 August 2023 6,828 2 View
I have a developed a scale. EFA is done. now doing CFA. The scale has 4 sub-scales which are correlated to eachother (ranging from .30 to .70). I need to know should I do first order CFA or second...
19 July 2023 7,745 3 View
on the basis of memories, identities, power relations, and the city's genealogy
08 June 2023 2,304 0 View
Can any one kindly let me know the normal, mild, moderate, and severe levels of lead and arsenic in Urine ?
20 May 2023 5,798 0 View
CNV analysis identifies malignant epithelial cells from the single cell data of cancer tissue. Should CNA_value and the correlation between all cells and the top 5% of epithelial cells with CNV...
22 March 2023 5,929 0 View
At present many scientists are working on plants and their products without isolating them as pure and single molecule. Actually if plant products are consumed by living organisms they may be...
10 March 2023 7,758 0 View
I stained one cryovial PBMCs according to the CFSE protocol (invitrogen C34570). One day before staining, I thawed the cells and rested them in media (ingredients is below ) for 24 hours. On the...
02 March 2023 4,708 2 View