Contact experts in Next Generation Sequencing to get answers
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Questions related to Next Generation Sequencing
Where can we look for normative values on Pressure Pain Thresholds (Somedic algometer, suboccipital & tibialis anterior muscles) in asymptomatic adults?
06 February 2023 7,688 0 View
I have recently been attempting to isolate RNA from polymer scaffolds using the Dynabeads mRNA DIRECT Purification Kit. When I measure using a nanodrop like I would for other extraction methods...
03 January 2023 2,457 3 View
Hello, I am researching the phylogenetic tree of snakes. (bayesian Inference tree) My sequences are 1300 bp, but sequences of outgroups are about 600 bp. Can I change the difference of about 700bp...
12 December 2022 9,280 1 View
Hello! I am currently conducting experiments in which I am staining myofibroblasts and myotubes. To distinguish cell types I would prefer to have a multicolour staining comprising of 4...
29 November 2022 9,134 2 View
Hi, I'm interested in learning QSAR and docking but I don't know which software is versatile, and have a good support and community (preferably free). Any good recommendation? I'm working on...
14 November 2022 4,938 3 View
Hello I am trying to find out the exact binding sites of a number of peptides against a specific protein target. I did docking using many different servers and obtained many divergent results. How...
13 November 2022 3,079 3 View
I've started doing sanger sequencing recently and only rarely get good results. Every run I do barely has >100 Q20 and always has weak signal and excessive background noise. I've attached an...
11 November 2022 2,349 2 View
Hi, I 'm new to MD simulation. I was performing replica exchange method from Amber tutorial. In the tutorial developers uses Amber16 and they get the remd.mdcrd* files in human readable format...
09 November 2022 4,589 1 View
Hi everyone, I am looking to get some human insulin for cell culture work I have planned - I've ordered from Sigma but it won't get here till Christmas and I would rather get started sooner if...
02 November 2022 8,781 1 View
the equation is look like the below. Immun Factor =~ L activity + Cel M Im + IgT.2 + IgY.2 + IgM.2 + IgT.1 + IgY.1 + IgM.1
25 October 2022 7,913 6 View
i would like to know what is the meaning of transcriptomic analysis, how it can help in studying the mechanism of action of drugs against resistant microorganisms
23 October 2022 2,302 2 View
Hello I have to enlarge divergence point in phylogenetic tree. because It is difficult to identify the node because there are few mutations and it is too close. (Black dots are nodes)
22 October 2022 5,043 1 View
All the information I know so far is, A relaxed molecular clock is used, the mutation rate is 1.5% per mya, and normal distribution prior and GTR+I+gamma model. how do I setting priors and rate...
17 October 2022 9,091 0 View
Hello everyone. I am trying to do a molecular dynamics simulation on a docked complex of a protein receptor and a peptide ligand. However, I have encountered a major problem in the pressure...
11 October 2022 5,285 0 View
I am beginner in western bloting in our lab there is no gel doc machine so can I keep my membrane in PBST at 4 degree for a week and take image after week going to another lab will it work ?
09 October 2022 2,803 3 View
I am constructing a phylogenetic tree using align sequences of 4 mt-genes [2 coding, 2 noncoding]. Total nucleotide length extends up to about 2500bp. I am stuck in one point, therefore I want to...
23 September 2022 2,114 1 View
The molecular clock will be analyzed using a gene region with little variation between populations. which one I have to use, BEAST or mrbayes? and tell me why. thanks.
16 September 2022 3,439 0 View
We have a GWAS population (genotyped) and we want to select the minimum (an optimal) set of genotype to phenotype in order to detect GWAS signals. Any soft or method available?
09 September 2022 3,639 2 View
if i have the enzyme activity of pectinase (8000-12000 U/g) and cellulase(>= 10 U/mg solid). then how to calculate that particular activity on extraction of lycopene from tomato
01 September 2022 3,494 2 View
the standard graph of p-nitrophenyl acetate, how we can make it, which concentrations can be used?
05 August 2022 828 0 View
GBS
21 July 2022 5,887 0 View
I was wondering if anyone had issues with their MiniAmp. Overall, I'm quite happy with the instrument but I find the lid heats/cools extremely slowly (0.3 degrees/sec) and I cannot find any way to...
08 July 2022 7,467 0 View
I want to know how no template control looks with single probe mix. I am seeing a prominent band just above 50 bases when tried with a single probe mix (targeted to one gene) and multiple faint...
19 April 2022 3,836 0 View
In certain industry, there is a huge amount of Calcium Sulphate produced annually as a subproduct. Is there any idea for how to use them or it will be sent to the landfill???
05 January 2022 4,739 6 View