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Questions related to Next Generation Sequencing
the standard graph of p-nitrophenyl acetate, how we can make it, which concentrations can be used?
05 August 2022 789 0 View
I want to know how no template control looks with single probe mix. I am seeing a prominent band just above 50 bases when tried with a single probe mix (targeted to one gene) and multiple faint...
19 April 2022 3,811 0 View
In certain industry, there is a huge amount of Calcium Sulphate produced annually as a subproduct. Is there any idea for how to use them or it will be sent to the landfill???
05 January 2022 4,710 6 View
Hi, I want to do GO and KEGG analysis using DEGs/Gene IDs to do GSEA and ORA by ClusterProfiler pipeline in R. As my organism (Brassica napus) is not a model plant, so I cant use R supported...
16 November 2021 1,272 1 View
In our laboratory, different results were obtained in the MLPA test performed for a gametic mutation on the peripheral blood samples belonging to the same patient with an interval of one year. We...
18 August 2021 445 3 View
I'm about to analyze the intracellular Ca+ concentration in HT29 and SW480 cell lines using flow cytometry. I am thinking of using Fluo-4 AM (invitrogen) but I am not sure which would be a good...
14 July 2021 1,383 2 View
Hello to you all, I want to perform DNA seq and I am having issues in the step of ligating the adaptors to the sequences. To give you more details: We want to find the different virus detected...
01 July 2021 5,626 0 View
I am having an assembled fasta file (~3.7 GB in size). I tried using the web based MISA v2.1, but it accepts fasta upto 2Mb only. Is there any other alternate software which can be used for a...
13 June 2021 5,746 5 View
Also can anyone tell me, how to interpret incase germline mutations in oncogene like ALK OR MET genes were found??
26 April 2021 823 0 View
1. Can the DNA mutations that do not affect the phenotype be used as the markers to develop human genetic maps? 2. Can the human genetic maps be developed based on DNA mutations that affect phenotype?
04 April 2021 4,323 3 View
We normally use an agarose overlay but would like to test avicel. We carried out the plaque assay as normal and infected half of the wells and left the other half as a control. Upon removal by...
05 March 2021 7,322 1 View
I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...
18 February 2021 7,738 2 View
We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...
17 February 2021 5,681 4 View
I would like to barcode fungal caps from the field for two main research goals (1) molecular iD of the fungal specimen and (2) to identify any invertebrate DNA within the cap using CO1...
09 February 2021 4,580 1 View
I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.
03 February 2021 8,488 3 View
I want to mainly identify long nc RNA.
26 January 2021 5,970 2 View
I am culturing SVG p12 cells in %10 FBS, %1 Pen/Strep DMEM medium. But whenever I passage them into a much larger flasks they are began to de-attached and started to die. I couldn't find any...
17 January 2021 4,741 4 View
I have some sequences of Spike protein of some Covid 19 samples that have been sequenced in my laboratory using NGS. For analyses of those, I need to convert those sequences into PDB format. I am...
02 December 2020 794 3 View
Hi Everyone, I have a list of 2000 SNPs (chicken)and require to find close genes to all these 2000 SNPs. I used to do that one by one in a small set of SNPs by using org.Gg.eg.DB, BiocManager,...
30 November 2020 1,978 8 View
Hi there, I am working on airborne microbes. I want to know the concentration of bacteria in my sample before I go for NGS. Since it is an environmental sample it might have a lot of mixed...
18 November 2020 460 3 View
I have about 30 blood samples from people with inherited fructose intolerance who need to use the next generation sequencing technique. Is there a research center or person doing this test to work...
03 November 2020 3,594 3 View
I would like to isolate T cells from mice with inflammatory disease and perform single cell RNA sequencing and detect the cytokine mRNA profiles. do I need to stimulate T cells with PMA and...
31 October 2020 6,069 2 View
I am doing an analysis with transcriptome data and comparing the same with the Genomic data. More precisely, I am looking at mRNA results of a tumor sample without having the normal tumor samples...
27 October 2020 3,910 3 View
Dear all. We need to sample insects, store them upon collection and ship them internationally. Sampled will later be processed for DNA extraction and NGS sequencing. Keeping all this in mind, can...
26 October 2020 7,508 2 View