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Questions related to Next Generation Sequencing
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
22 July 2020 2,113 4 View
Hi. I received the fastq files for my ChIP-seq studies. We did 50 bp paired-end. I am new to the NGS. I used paired-end data from public files and usually, there were only two fastq files for...
14 July 2020 4,243 0 View
I have mutated a gene in the zebrafish and believe there is a phenotype in homozygous mutants, albeit a subtle one. I am able to pick out mutants at ~4--5 dpf based on a slight bend in their...
03 July 2020 1,845 3 View
Last night, the fridge (-20°C) in our laboratory didn't work well. It was not cooling and the temperature rose up to room temperature. The DNA ligase kept in that fridge. Nobody notices until next...
02 July 2020 3,430 0 View
Hi Dear colleagues I've got a FASTQ file that is produced using Thermofisher Ion S5 machine, I want to analyze this raw data using NIPTMer software: Main article: NIPTmer: Rapid k-mer-based...
28 June 2020 3,038 0 View
With the criterion, eigenvalue greater than one I have run factor analysis in SPSS for six items. KMO and Bartlett's tests are significant. First component is 2.988 with 49.795 % variance. Second...
26 June 2020 2,986 1 View
What I read form various literatures that development of tools happen in 3 stages 1) planning & deciding objectives 2) drafting pre tryout - apply on small small ~20 here one looks for...
18 June 2020 3,627 0 View
i have 8 factors. I would like to factor analysis using samrtpls. I need guidance in drawing model path daigram for doing factor analysis for 8 factors
12 June 2020 6,217 4 View
I am interested in sequencing the transcription start sites in human cells in order to see mutations under a certain condition. I know that exome sequencing is able to get a few basepair reads...
11 June 2020 3,224 0 View
I am conducting a factor analysis for different items under one variable. Theoretically, they should into three categories (substantial, participation and accountability). The factor analysis that...
11 June 2020 517 0 View
Hi We've got an Ion S5 System (Thermofisher NGS System) at our lab, but suddenly we lost the SSD's (for electrical problems) and now we need to re-install the ubutnu (server and client) and all...
10 June 2020 2,858 1 View
My ancestors originated in Lorrha and my father was born in Ballymacegan in 1900 and died in 2000. I am interested in the ruins of the old McAoghain Law School which was adjacent to the...
08 June 2020 7,955 0 View
Dear All, I am investigating a new mutagen and its effect on C. elegans DNA. Is it any strains of C. elegans that can help me with fast screening of C. elegans SNPs? Can anyone suggest another...
05 June 2020 5,647 0 View
Is there any treatments for this disease? A 11 months old child was just diagnosed with Schindler disease type 1. The parents are really concerned. There are only 20 cases in the world with this...
02 June 2020 741 0 View
I want to sequence the amplicons with two different library prep kits. 1. Nextera DNA Flex Library Prep. kit 2. QIAseq Ultralow Input Library Kit Can I use the libraries from both preparation...
01 June 2020 1,896 3 View
I am looking for explanation in the context of NGS variant calling pipeline
28 May 2020 1,164 1 View
We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...
25 May 2020 8,305 3 View
Hi, I am trying to align two protein sequences using MEGA so that I can then use the alignment for homology modelling. This is a new area to me however a bit of reading tells me that a good...
22 May 2020 4,023 5 View
Greetings! I am dealing with NGS data. I have a vcf file of the called SNPs. I want to do population diversity and differentiation analysis with it but unfortunately, I unable to do so though I...
13 May 2020 5,992 3 View
My group would like to perform sequencing through Next-Generation Sequencing (NGS) in SARS-COV-2 isolates. However, although many groups have used this technique recently in their research, the...
29 April 2020 4,644 3 View
My team and I are currently trying to develop primers and probes for a multiplexed RT-qPCR assay. We were contemplating using the TaqMan QSY probes for quenching purposes, but are unsure if there...
24 April 2020 5,498 0 View
we are generating a proposal for detection of covid in our state, we need a quote and they have not answered me from NEB
19 April 2020 2,480 0 View
Yesterday I published "GCBS Bibliographies: Sources and Applications" on ResearchGate. However, I failed to request assistance in expanding the bibliography of applications. If you know of other...
16 April 2020 5,155 0 View
I saw this cross in an NGS video and photographed the screen, but I did not learn where this cross was found. Seems to me that it was found in some island. In Cuba there is one very much similar...
26 March 2020 2,468 2 View