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Questions related to Next Generation Sequencing
Hi, I am working with NGS data and I want to run my tools and pipelines on Ubuntu 20.04.1. Is anybody know how much disk space I need for working with following scenarios? 1. Processing the FASTQ...
15 February 2021 8,955 4 View
I would like to barcode fungal caps from the field for two main research goals (1) molecular iD of the fungal specimen and (2) to identify any invertebrate DNA within the cap using CO1...
09 February 2021 4,764 1 View
I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.
03 February 2021 8,698 3 View
I want to mainly identify long nc RNA.
26 January 2021 6,095 2 View
I want to compare OR of responses to the same question from two different time periods. the data source is a longitudinal study, which will have some participants included in both time periods,...
18 January 2021 5,502 5 View
I am culturing SVG p12 cells in %10 FBS, %1 Pen/Strep DMEM medium. But whenever I passage them into a much larger flasks they are began to de-attached and started to die. I couldn't find any...
17 January 2021 4,891 4 View
To check whether my desired proteins are on the same chromosomes or different chromosome, Whether they are near by in location or far. Kindly suggest me some databases that can give me these...
08 December 2020 2,536 4 View
I have some sequences of Spike protein of some Covid 19 samples that have been sequenced in my laboratory using NGS. For analyses of those, I need to convert those sequences into PDB format. I am...
02 December 2020 1,039 3 View
Hi Everyone, I have a list of 2000 SNPs (chicken)and require to find close genes to all these 2000 SNPs. I used to do that one by one in a small set of SNPs by using org.Gg.eg.DB, BiocManager,...
30 November 2020 2,146 8 View
Hi there, I am working on airborne microbes. I want to know the concentration of bacteria in my sample before I go for NGS. Since it is an environmental sample it might have a lot of mixed...
18 November 2020 764 3 View
I have about 30 blood samples from people with inherited fructose intolerance who need to use the next generation sequencing technique. Is there a research center or person doing this test to work...
03 November 2020 3,829 3 View
I would like to isolate T cells from mice with inflammatory disease and perform single cell RNA sequencing and detect the cytokine mRNA profiles. do I need to stimulate T cells with PMA and...
31 October 2020 6,155 2 View
I am doing an analysis with transcriptome data and comparing the same with the Genomic data. More precisely, I am looking at mRNA results of a tumor sample without having the normal tumor samples...
27 October 2020 4,087 3 View
Dear all. We need to sample insects, store them upon collection and ship them internationally. Sampled will later be processed for DNA extraction and NGS sequencing. Keeping all this in mind, can...
26 October 2020 7,699 2 View
I am looking for opinions/recommendations of NGS Platforms (Illumina vs. BGI/MGI) from people that had opportunity to work on both hardware: MGISEQ-T7 and NovaSeq 6000 or analyze data from both....
05 October 2020 8,395 1 View
Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...
29 September 2020 2,694 1 View
Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.
28 September 2020 1,191 2 View
I am analyzing a fish gut (16S rRNA gene) microbiome dataset. I have some continuous variables (e.g. weight, blood cortisol levels) and want to see if there is any correlation between bacterial...
03 September 2020 7,090 3 View
Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...
26 August 2020 8,753 1 View
I ordered 16S primers according to the earth microbiome website, however, the researchers added GCT to the end of the Forward primer Illumina 5' Adapter to affect the melting temperature of the...
14 August 2020 1,946 1 View
Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...
12 August 2020 786 3 View
I wanted to learn R and Python for genomics work and i have experience in using GUI platforms like Galaxy and CLC for NGS analysis. I am now looking to expand my knowledge in coding based NGS...
01 August 2020 6,947 9 View
Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance
28 July 2020 10,135 5 View
what companies can provide Next generation sequencing services for free or at the lowest cost?
26 July 2020 6,864 3 View