A platform to discuss the methods and technologies involved in DNA sequencing. | Contact experts in DNA Sequencing to get answers
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Questions related to DNA Sequencing
Dear All, I would like to make my own native gels. Have recipes on Tris-Glycine running buffer and TRIS Glycine Native sample buffer. Would like to make TRIS Acetate gels, discontinous, 7%. Cannot...
17 August 2023 5,158 0 View
Does anyone have a recipe to make a non-denaturing gel buffer containing typical gel dyes plus glycerol. I'm specifically looking to use Bromo phenol blue or xylene cyanol blue but anywhere to...
29 November 2021 3,964 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
04 March 2021 4,246 1 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
25 February 2021 4,810 3 View
I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...
30 January 2021 6,417 4 View
Recently I faced a problem while separating 3 species of genus Lethe butterflies (group Woodbrowns -Satyrinae) working along an elevation gradient and different forest habitats in Kedarnath Musk...
16 October 2020 3,111 3 View
I have mitochondrial DNA sequences to perform analysis of the genetic diversity and population structure of a fish species. What packages can I use on R? I estimate that I need to generate...
02 October 2020 1,025 5 View
I would like to design primers that are complimentary or begin in the UTRs (5' and the 3') of a gene? Where exactly and how do I find the coding sequence that includes the UTRS ?
29 September 2020 4,972 4 View
I am very new to this field and I hope you could share with me if there are any instance that this actually happens or has been reported in the past? If so, what are the usual interpretations or...
29 September 2020 1,456 5 View
I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.
26 September 2020 8,257 5 View
Hi, After comparing multiple (2+) DNA sequences, I want to identify ONLY the restriction sites that changed due to the detected polymorphism. I tried several things by hand, but it's WAY too...
24 September 2020 6,621 4 View
I wonder if it is possible to infer the origin of invasive lineages based on genetic data for many native and invasive populations. I first planned to use DIYABC v2.1.0, but I am not used to...
23 September 2020 7,993 3 View
Hello. I am planning an RNAi experiment. I want to silence my gene of interest using RNAi. My gene of interest is crucial for plant development. Also, my gene of interest is known to function even...
22 September 2020 3,383 3 View
Hello ! How can I eliminate primer dimers accumulation in pcr? Thanks in advance.
14 September 2020 2,132 8 View
Hello, I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end) Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1) I have done...
11 September 2020 8,307 3 View
Oligonucleotide primers are used to generate bands corresponding to their own gene in PCR assay.
07 September 2020 2,283 7 View
When analysis bacterial DNA sequences the consensus doesnt match the reference sequence, when blasting them. Many bad psectra are viewed in the analysis software. Please what to do in this case?
29 August 2020 2,238 9 View
Hello, I extracted the DNA of my each 13 inbred mice of C57Bl/6J strain, before and after the behavior experiment, 26 samples in total. During shipping the mice I have lost their ID given before...
26 August 2020 5,793 4 View
I want to perform some statistics on the lengths of DNA-Sequences inside NCBI Genbank, for example, I want to draw a histogram of these lengths. So I am asking is there a way to retrieve the...
22 August 2020 8,708 3 View
Hi, I need Seqscape software 2.7 to analyze DNA sequences because this version supports 3500 sequencers. Please help me how can I access this version of the software? Thanks
18 August 2020 4,920 3 View
Hello everyone, is there a tool that could allow me to align protein sequences to genomes and works also well with shotgun genomes? I have Geneious prime, that allows me to align scaffolds from...
06 August 2020 2,676 4 View
Can I digest a specific DNA sequence without a known restriction enzyme site in vitro? I am trying to digest a fragment with CGTCAT but not CATCAT. I tried cas9 with a sgRNA containing the target...
21 July 2020 9,573 4 View
I have the Illumina reads and assembly of my bacteria from pure culture DNA sequencing and the nanopore sequencing data from the metagenome (DNA of co-culture). Does anyone know the best way to...
15 July 2020 6,724 3 View
When i submit dna sequences to NCBI, they ask for clarification saying "Some or all of the protein coding sequences contain internal stop codons, reading frame shifts (insertions/deletions...
04 July 2020 6,445 1 View