What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

Western blots on tissue which have been stored in RNAlater?

I have used up my snap frozen tissue from an experiment but have some left in RNAlater (invitrogen). I was wondering if anyone had any experience with running western blots using tissue that has...

28 August 2019 7,930 3 View

What are the best conditions for transferring a >200kDa and ~40kDa protein?

I am having issues transferring my large protein (220kDa) at 30V for 2 hours (RT). It is often patchy with incomplete bands (annoyingly I occasionally I get a clear transfer!). For my other...

22 August 2019 1,081 7 View

Whats the best way to extract and save venom from a live lionfish?

I'd like to pull venom from my pet lionfish. I can imagine that pressure and puncture of a basic seal on a beaker or something will work, but is there a good type of seal to use? and how should I...

21 March 2017 9,231 5 View

Does anybody know what the protein turnover/resynthesis rate/lifetime is for RORgt?

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05 December 2013 9,870 2 View

Any tips or help with Matrigel in vivo?

I plan to subcutaneously implant cells into mice using Matrigel, however this is my first time doing so. Does anyone have any guidance regarding the concentration on Matrigel to be used with...

02 July 2013 7,857 47 View

Adherence problems with the hOST TE85 cell line after adaptation with knock out serum.

I have adapted my hOST TE85 cells to a media with no FBS in just a knock out serum replacer. They adapted well and grew steadily. I then froze them down in 10% DMSO, 10% FBS and 10% DMSO and 10%...

13 January 2013 6,901 14 View

Nicholas have you read the paper by Max Kurz et al 2014 in DMCN?

It is regarding brain image of self in CP. It suggests a mechanism which may explain why the use of DEFOs can have an effect on the management and development of childhood sitting and movement...

01 January 1970 4,665 1 View

Have there been studies linking spatial reasoning skills with numeric cognition in unschooled/illiterate adults?

01 January 1970 1,944 4 View

What is your strategy to complete?

Hi Keith, I am working similar goal, but more in knowledge management training. Hope to compare strategies sometime. Cheers Karl

01 January 1970 2,759 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View