I'm about to analyze the intracellular Ca+ concentration in HT29 and SW480 cell lines using flow cytometry. I am thinking of using Fluo-4 AM (invitrogen) but I am not sure which would be a good positive control for this assessment. I've read that Thapsigargin could be used for this purpose but any other recommendation, general tip etc are more than welcome.

Thank you in advance!

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