Contact experts in Next Generation Sequencing to get answers
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Questions related to Next Generation Sequencing
Hi everyone, I am new to the NGS data analysis. I want to trim my raw ngs data to remove junk adapters using Cutadapt package. Now I have two questions. 1.How can I obtain the sequences of the...
02 January 2019 8,179 7 View
when I constructed library for Next-generation sequencing, the efficience of adapter ligation was low, How can I improve it? chage the enzyme or the condition of reaction.
28 December 2018 7,774 3 View
Hello, In my pipeline for genealogy analyses (diploid SSR, 9 loci, 3pop, 108 individuals), I used DIYABC ver.2.1. 15 scenarios total; pre-eval rejected 8 of them. I want to chop out those...
20 December 2018 4,002 0 View
i am running factor analysis in r and the factanal function gives me an output with some factor loading entries missing
04 December 2018 4,691 4 View
Please suggest me a pipline along with generation of heat maps and networks.
03 December 2018 7,983 3 View
R producing two different results for factor analysis. Pl have a look and let me know if you can. Thanks in advance.
03 December 2018 7,911 7 View
Hi, we have two Illumina-sequenced plant samples where one is a mutant of the other. Do you know a straightforward method to compare these in order to spot small (SNPs, InDels) or maybe longer...
21 November 2018 5,958 4 View
Hi everyone. I am seeking to validate the effect on splicing of a TP53 somatic mutation identified with NGS. I am going to sequence the cDNA derived from tissue specimen and I was wandering to use...
06 November 2018 3,624 2 View
Several of the genes in our NGS-panels have psuedogenes (some genes have only 1 pseudogene, others have 14). Due to various degee of sequence homology, this will influence the mapping quality and...
26 October 2018 1,665 1 View
I extracted viral RNA samples using Qiagen Viral RNA kit. the samples gave concentrations average 30 ng/ul and purity of average 2 for both 260/280 and 260/230. I shipped the samples using RNA...
21 October 2018 1,998 5 View
454 GS FLX Titanium system which is capable of generating 700 megabase (Mb) of sequence in 700 bp reads. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 ×...
18 October 2018 2,364 3 View
Next generation sequencing (NGS) is a wonderful tool for identifying waterborne pathogens. But I was wondering what are the challenges we face in NGS in terms of identifying the correct pathogen...
11 October 2018 467 2 View
I'll preface this by saying our NGS Core facility handles most of our analysis. So perhaps in my limited understanding of the analysis workflow, I'm not correctly articulating what I'm looking to...
27 September 2018 10,090 4 View
So I am trying to proof that a specific TF binds to my gene. I have done this by ChIP. Worked all fine. Now I wanted to delete the TF binding site via CRISPR/Cas9. Unfortunately (and i have...
24 September 2018 2,486 6 View
If I am interested in a customized mRNA-only Sequencing (RNA-Seq) based on few transcripts (not the whole transcriptome), and after having satisfied & permissible RIN values for my extracted...
22 September 2018 1,908 3 View
Hi everyone, Can I use the NGS method to study the effect of a bacterial secondary metabolites on wheat? Do you have a reference article? Thankful,
22 September 2018 1,148 3 View
Please mention in details the advantage and disadvantage of any particular software if you recommend. Thanks in advance.
12 September 2018 8,492 6 View
Any tool or package to prepare input files for Circos visualization of NGS data? I know of some, but not very user-friendly.
11 September 2018 668 2 View
Hello, I am looking for a company or a lab that creates T-cell lines with specific mutations? Does anyone know where I can find such a thing? I am trying to find ones with mutations in...
05 September 2018 9,114 1 View
Hi We've just started using the Illumina Next-Seq platform and haven't been getting good results sequencing our CEL-Seq2 library (75cycle, R1=15bp, R2=77bp), as opposed to what we've been getting...
24 August 2018 7,609 7 View
Next Generation Sequencing techniques are currently used in microbial profiling and other studies. Between the NGS (specifically Illumina Miseq Technique) and PLFA, which is the best method to...
23 August 2018 2,352 3 View
I worked in Nancy from 1985 to 1988 and from 1990 to 1991-1992. Then I returned to Paris ( LPS Paris ) in a quasi -closet . I survived with a temporary collaboration with G tyuliev .... I...
16 August 2018 3,425 1 View
Is there any other software to plot TADs ( I have chromosome start and end position), normalized tag count from ChIP-Seq data, and log2FC of genes (fold change from RNA seq data) in one graph?
09 August 2018 1,175 1 View
Apart from amplification requirements being mandatory for NGS, what are the other differences or advantages third generation sequencers like Oxford nanopore and PacBio provides? Which one is more...
07 August 2018 6,044 3 View