Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
04 April 2019 7,903 5 View
Hi, I am trying to do a correlation study with miRNAs and its target transcript. The problem lies in the fact that the normalized counts for miRNAs are obtained from DeSeq2 whereas the normalized...
04 April 2019 4,296 1 View
There is a lot of research on using exosomes in nanoplasmonic sensors but could we use microRNA instead and whether the sensitivity would increase or decrease??
04 April 2019 931 2 View
When I studied miRNA expression in normal tissues/cells the control that I used was U6 snRNA. Does someone know if this could be used also with breast cancer cells to estimate miRNA expression in...
04 April 2019 2,033 4 View
I seeded PC3 cells in 2 T75 cm2 flasks (8 x 106 cells/T75). After incubation overnight with 5% FBS-Depleted EVs medium, I isolated exosomes from cell culture with a Qiagen kit and I performed a...
04 April 2019 1,543 1 View
02 April 2019 7,078 1 View
please help me, I am given numerous sequence & I need to extract features out of it, so that I can study the miRNA-mRNA interactions by focusing on miRNA targets.
02 April 2019 521 2 View
Dear Researcher I need some suggestions and literatures about the regulatory role of miRNAs in adipocytes. Most of the literature i searched shows, that if a miRNA inhibit proliferation it will...
09 March 2019 6,808 6 View
I am searching for miRNA database that contains the nucleotide sequences of cancers in the human genome. I have found a database that characterizes the samples by the number of reads_per_millio...
08 March 2019 9,759 3 View
I want to use mimic of miR-124 on the specific vehicle in the mouse model of CRC, I wonder if there is any principal difference between the various forms of this miRNA mimic (LNA and other forms)...
03 March 2019 6,081 3 View
Is there anyone who can share proper method of qPCR especially for microRNAs?? Thanks
03 March 2019 1,213 2 View
I am using miRNA LNA primers with SYBR Green. Melting curves and peaks are shown in Fig1. Do I have to run gel analysis too? If so, what kind of ladder should I use? Any experiences in gel...
03 March 2019 9,284 3 View
03 March 2019 6,903 7 View
03 March 2019 2,255 4 View
So I am performing a biomarker (piRNA based) study in a human solid cancer. Based on the initial analysis, I have shortlisted a few piRNAs - but I am stuck with two issues: 1. A few piRNAs have...
03 March 2019 4,333 1 View
Hi, Does anybody know the Invitrogen miRNA Wash Solution composition? Thanks
03 March 2019 6,183 0 View
Hello! I am a young CONICET researcher at Buenos Aires University, Argentina. My main project is "New drugs against triple negative breast cancer". I am looking for a US laboratory that could...
03 March 2019 2,672 0 View
Hello,I would like to design the mimics and inhibitors of miRNA.According to the mature miRNA sequence ,what should I do ?Thank you very much.
03 March 2019 6,254 1 View
is rabbit no longer use or representative for atherosclerosis model?
03 March 2019 171 1 View
Because of lipids in postprandial blood, I have some troubles to extract microRNA. How can I remove lipids without alter the quality of microRNA?
26 February 2019 2,895 3 View
Hi everyone, I am trying to analyze the miRNA-Targets of the FOSL1 gene but I am facing a particular problem. With Targetscan 7.2: -> 2 different miRNA Targets for FOSL1 got predicted. The...
11 February 2019 2,719 2 View
Hi all, We are planning to see if there are miRNAs that targets 3' UTR of genes and if there are miRNAs coded in intronic regions of these genes of interest in Dicentrarchus labrax. My question...
11 February 2019 8,002 2 View
Recently i was able to introduce a mutation in a protein using CRISPR. sequencing confirmed the mutation. WB showed significant decrease in the mutant protein. in order to determine the protein...
02 February 2019 7,623 5 View
Greetings, We are conducting an experiment after which we will measure the levels of certain microRNAs in the hippocampi of albino rats. What would be the best housekeeping/reference gene to use...
02 February 2019 3,227 7 View