Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
2,271 views 2,540 posts
Questions related to microRNA
Interesting research on coping strategies of adolescents with cerebral palsy
08 January 2021 5,990 2 View
Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...
01 January 2021 7,731 5 View
I have screaming data, and I have to analyze it with PIC(Phylogenetic independent contrasts). I used logistic regression to see the relationship with other variable, but it cannot be applied...
29 December 2020 8,612 0 View
I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...
27 December 2020 10,313 2 View
Can I ask the expert in this page: do we have any studies about the COVID Vaccines for those with AutoImmune diseases like SLE or RA, and also for those taking Immunosuppressant drugs ?
23 December 2020 9,000 6 View
Hello, I am doing SNP analysis for my gene. I have calculated SIFT and Polyphen score but I cannot find way to calculate REVEL score. Can someone guide me, how can I get this score? Thank you.
12 December 2020 2,333 7 View
I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...
08 December 2020 7,919 3 View
I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...
07 December 2020 8,714 3 View
Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...
07 December 2020 2,018 3 View
My research question is about a protein that is a homo dimer (Two monomer units having chain A and B respectively) Now, we are trying to see the mutational effects in both monomers (same...
03 December 2020 2,163 3 View
For example, can we use the microRNAs involved in specific stress in a plant submitted on mirbase.org as a source for analysis of those microRNAs in other stresses? Thank you in advance for your...
01 December 2020 5,500 3 View
Performing pulsed Doppler ultrasound on a single thyroid artery (upper or lower; right or left) is it sufficient for the study of the mean sytolic peak in order to differentiate Graves' disease...
01 December 2020 4,208 3 View
I'm looking at wild horse populations and trying to map their relations. Ultimately I'd like to assess their relatedness and inbreeding coefficients. However, the most important component right...
30 November 2020 5,502 3 View
I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...
27 November 2020 9,716 5 View
We Want to do CRISPRa of a long noncoding RNA. But after going through the literature I am still not sure how to identify the promoter to design the sgRNA. How to approach the problem? Thank you.
26 November 2020 180 2 View
I have no prior knowledge of microRNAs and a member of my lab just started her project working on it. ive done primer designing before for my genes but it cant be applied to designing primers for...
24 November 2020 3,152 4 View
I'm trying to determine amount of RNA extracted from zebrafish embryos (using Qiagen micro kit) and whether it is sufficient for cDNA/qPCR. Based on nanodrop output I appear to have an ongoing...
24 November 2020 8,999 2 View
For example for an interaction study between a protein target.
23 November 2020 4,330 4 View
Hi! I would like to know what tools I could use to analyze interactions between a new lncRNA and possible protein/miRNA targets. The biggest problem I had to find such tools is that the RNA we...
21 November 2020 8,636 3 View
I am going to do field research on amaranthus. If anybody could help me with some ideas about the probable study to include in research work.
17 November 2020 3,843 3 View
Hello everyone, I'm looking for miRNA expression level data (as measured by RNA-seq) in a specific tissue, but all papers I have consulted (and their relative supplemental material) do not report...
15 November 2020 10,016 2 View
Suppose we have given miRNA (that according to in silico analysis should bind to a specific sequence in RNA) that is labeled with a fluorescent dye (or is labeled otherwise) and we have isolated...
13 November 2020 5,457 1 View
I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...
12 November 2020 5,695 1 View
Hi Chandni Bansal, first congratulations by your work. I have 3 questions: Have your checked its target AP2 for MIR172 in tomato plants as positive control of the technique Is the double mutant...
11 November 2020 1,368 0 View