Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
Imagine that we have point interacting particles, that moving according law like x(i, t+dt)=x(i,t)+f(i,j,t)*dt. Or also velocities can be included. In the limit dt ->...
23 October 2019 9,760 5 View
I was wondering if I can get some ‘hands on experience’ help with my Southern Blot experiments. I have been failing in getting anything other than a high background blot image (see picture) at the...
22 October 2019 6,071 3 View
1. O.D 0.1strain C58C1 2. Antibiotics used: Cefo. 500mg/l, Carbencillin 500mg/l, Timentin 500mg/l, amoxycillin 500mg/l + all possible combinations. 3. While making selection medium filter...
19 October 2019 1,995 1 View
I'm trying to isolate tomato protoplasts for gene editing, and most references that I've found suggested to keep the plants grown the culture room to be kept in the dark for 3-4 days before being...
14 October 2019 6,092 4 View
We are analzying changes in miRNA expression as a result of virus infection using Nanostring. While I have a lot of interesting hits in my data, I do not know the best way to determine the...
10 October 2019 8,528 3 View
I used Qiagen miRNEasy micro kit, miScript RT II kit and miScript SYBR greenPCR kit with Qiagen custon primers assays for mir-499 and mir-208. No-matrix PCR showed one-peak melting curve and the...
10 October 2019 9,039 3 View
Failure of immune response to the fungal infection is the major cause of invasive fungal infections (IFIs). MiRNAs are the master tuners of immune system.
10 October 2019 7,890 4 View
I'm more focused on the miRNA than RISC. Does the miRNA degrade after cleaving mRNA?
10 October 2019 9,337 1 View
I want to perform miRNA qPCR for several micro-RNAs from Gallus gallus. Which genes should I use to normalize the results?
10 October 2019 2,831 2 View
I am looking to understand the mechanisms by which siRNA works to inhibit RNA production versus microRNA does anyone know the average time these molecules stay active in the body? My preconception...
10 October 2019 551 3 View
In the article "MicroRNA miR-320a and miR-140 inhibit mink enteritis virus infection by repression of its receptor, feline transferrin receptor", the part of " Argonaute 2 (Ago2)...
10 October 2019 4,504 0 View
I am looking for databases that contain microRNA-drug interactions. Any suggestions or recommendations?
10 October 2019 5,339 4 View
Hello, I am a PhD student and during the analysis of sequencing data of miRNAs in the Galaxy project online tool an error occurred in the final stage of the analysis, when using the DESeq2 tool...
10 October 2019 8,911 2 View
08 October 2019 3,726 4 View
I am looking for some help in deciding on endogenous control genes for my Taqman assays? I would also value any input with regard working with fluids typically containing very low levels of...
08 October 2019 4,876 2 View
I am currently working on INS-1E cell line to study pro-inflammatory molecules as well as PDX-1 expression under high glucose. May I know what are the protein isolation protocols for these target...
23 September 2019 2,609 0 View
I am analyzing a data generated from an RT-qPCR experiment where different treatments, each with 3 different concentrations are applied (See attached snapshot). The CT value for around 50...
20 September 2019 8,314 5 View
To stain TMRM in the whole brain of adult Drosophila, I referenced several methods, ultimately following the method below: 1. Dissect 3 adult brains in cold Drosophila Schneider media (DSM) 2....
19 September 2019 8,140 0 View
I'm looking for a pET28 vector with cleavable N-terminal His tag. I noticed there are these two variants (pET28-a and pET-28b). May I know what the difference is between these two?
10 September 2019 7,268 6 View
Greetings, I am performing a reverse transcription using the taqman microRNA assay protocol. Parameters of thermocycler for this step are as follows: 30 min/16°C, then 30 min/42°C, then 5...
09 September 2019 9,456 3 View
We are looking for microRNA pull down assy for human cell lines.
09 September 2019 8,810 4 View
Dear Friends, I need guidance in initiating work on circulating miRNA - whole blood in PE patients. What are the requirements and how to collect the blood Required Protocols...
09 September 2019 7,893 4 View
09 September 2019 6,927 7 View
I conducted a miRNA PCR and I was wondering if miRNA that is bound to a mRNA is expressed in the results from my PCR. Thank you!
09 September 2019 7,499 7 View