Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I want to perform miRNA qPCR for several micro-RNAs from Gallus gallus. Which genes should I use to normalize the results?
10 October 2019 2,827 2 View
I am looking to understand the mechanisms by which siRNA works to inhibit RNA production versus microRNA does anyone know the average time these molecules stay active in the body? My preconception...
10 October 2019 547 3 View
In the article "MicroRNA miR-320a and miR-140 inhibit mink enteritis virus infection by repression of its receptor, feline transferrin receptor", the part of " Argonaute 2 (Ago2)...
10 October 2019 4,500 0 View
I am looking for databases that contain microRNA-drug interactions. Any suggestions or recommendations?
10 October 2019 5,329 4 View
Hello, I am a PhD student and during the analysis of sequencing data of miRNAs in the Galaxy project online tool an error occurred in the final stage of the analysis, when using the DESeq2 tool...
10 October 2019 8,908 2 View
I used Qiagen miRNEasy micro kit, miScript RT II kit and miScript SYBR greenPCR kit with Qiagen custon primers assays for mir-499 and mir-208. No-matrix PCR showed one-peak melting curve and the...
08 October 2019 3,721 4 View
I am looking for some help in deciding on endogenous control genes for my Taqman assays? I would also value any input with regard working with fluids typically containing very low levels of...
08 October 2019 4,873 2 View
I am analyzing a data generated from an RT-qPCR experiment where different treatments, each with 3 different concentrations are applied (See attached snapshot). The CT value for around 50...
20 September 2019 8,305 5 View
To stain TMRM in the whole brain of adult Drosophila, I referenced several methods, ultimately following the method below: 1. Dissect 3 adult brains in cold Drosophila Schneider media (DSM) 2....
19 September 2019 8,136 0 View
I'm looking for a pET28 vector with cleavable N-terminal His tag. I noticed there are these two variants (pET28-a and pET-28b). May I know what the difference is between these two?
10 September 2019 7,259 6 View
Greetings, I am performing a reverse transcription using the taqman microRNA assay protocol. Parameters of thermocycler for this step are as follows: 30 min/16°C, then 30 min/42°C, then 5...
09 September 2019 9,450 3 View
We are looking for microRNA pull down assy for human cell lines.
09 September 2019 8,800 4 View
Dear Friends, I need guidance in initiating work on circulating miRNA - whole blood in PE patients. What are the requirements and how to collect the blood Required Protocols...
09 September 2019 7,885 4 View
09 September 2019 6,923 7 View
I conducted a miRNA PCR and I was wondering if miRNA that is bound to a mRNA is expressed in the results from my PCR. Thank you!
09 September 2019 7,493 7 View
How to isolate microRNA from Blood samples? Please suggest an efficient way to do such with reference of a paper or protocol.
09 September 2019 3,240 3 View
I am trying to construct a standard plot for miR21 and miR 200 (Cq vs conc.) using synthetic oligos but even on using miRNA concentrations of upto 100pM I only begin to get any amplification...
09 September 2019 6,034 9 View
I am looking for any user friendly online tools for predicting the secondary structure for 3'UTR.
24 August 2019 2,372 7 View
I am looking to investigate miRNAs to compare expression between patients and healthy controls. The patients are likely to have abnormal lymphocyte counts. Can anyone advise on the preferred...
15 August 2019 8,410 2 View
Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...
14 August 2019 2,299 2 View
Hello, I'm wondering how to get a list of microRNA or lncRNA or other types of RNA that is associated with a certain type of cancer such as breast cancer? I see in research paper they often put...
08 August 2019 8,368 5 View
I have data of mRNA as well as mi-RNA. Can I do integrated analysis of these using SPSS ?
08 August 2019 2,011 3 View
Hi, has anyone experience of miRNA qPCR panels, especially QIAgen miRCURY LNA miRNA PCR panel? I performed a qPCR run of 384 well plate (panel I) from SW480 cell line according to the procedure,...
08 August 2019 5,451 7 View
I am trying to establish any existing relationships, if any, by performing analysis of miRNA mediated gene-drug regulation of diseases in humans. EDIT: By mathematical relationship I want to...
08 August 2019 2,691 5 View