Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
In B.subtilis spore surface display cot partner with native promoter is fused in frame with a passenger protein via a linker like GGGGS. The assumption here is that a fusion mRNA is formed, and...
01 March 2023 2,878 1 View
Ex. Shannon's Index is 2.45, is it high or low?
28 February 2023 1,243 1 View
Sex in hard ticks (Ixodidae) is very easy to determine, but does anyone have a reliable method to determine the sex in soft ticks (Argasidae)? Thanks in advance. Manuel
17 February 2023 5,899 2 View
Physics incests billions in theory development and experiments to the problem of quantum theory-GR (general relativity) unification. Some hypotheses like holographic principle emerged born out of...
02 January 2023 5,939 4 View
I would like to get a script written in biopython that creates a list of genome accession numbers for the genus Pseudomonas. But I don't know enough about biopython to achieve this. The goal is to...
29 December 2022 4,468 5 View
Classical physics has always distinguished particles from waves. However, at the beginning of the 20th century, the newly discovered phenomena of atomic and subatomic particles as well as the...
23 December 2022 9,493 6 View
I am working with Pyromark Q24 protocol on bisulfite converted DNA that has been through a PCR and verified by gel. I have a very high substrate peak (~200-250) that is resulting in low nucleotide...
19 December 2022 8,757 1 View
I am doing fourplex mqPCR panel for pathogens by Taqman probe . I did well choose for different dyes option (reporting dyes). real time PCR is five channel 7500 applied biosystem. For primary...
12 December 2022 877 4 View
Hi everyone, Im quite new to co-expression network analysis and I've been wondering what would be the minimum time points I could use to create a meaningful co-expression network to analyze? I...
07 December 2022 6,077 1 View
The abstract is as follows: Title: Quality of life of Iranian adults with neurofibromatosis 1: validity and reliability study Abstract Aim: Evaluate the quality of life of Iranian patients with...
29 November 2022 7,194 2 View
Does anyone have experience working with Total RNA spike - ins for total RNA extraction & miRNA quantification with rt - qPCR? I have been made aware of the c.elegans spike in however i would...
22 November 2022 9,246 0 View
I need a detailed explanation of haploview use to study haplotypes association with the outcome. I have read many resources, but did not manage to change my excel data of genotypes into ped and...
05 October 2022 2,254 0 View
Hello i have been calculate some parameters of ROH in the summary List, like percentage, mean and count in Plink,...
20 September 2022 1,247 1 View
I transduced my B cells with GFP containing plasmids using lentivirus and achieved 60% positivity. However, when I checked my cells under the confocal microscope, I can not see any GFP signals....
13 September 2022 9,094 4 View
I know that miRNAs are stable up to 3 to 5 years in plasma/serum but I want to include CSF samples to my study. The problem is that these samples are difficult to come by. I already have some in...
20 August 2022 2,610 0 View
I have been performing qPCR but i am getting zigzag pattern for my amplification plot. What could be the reason?
18 August 2022 3,804 0 View
We want to prepare sophorose in our laboratory and we have beta-glucosidase enzyme. We want to use sophorose in a liquid culture media for the increment of enzyme production.
07 August 2022 5,193 0 View
Hello, Recently I’ve been working on periplasmic expression of his-tag fusion protein. I’ve been struggling with 2 different manual purification that I’ve been found. Which protocol is better to...
19 July 2022 7,785 6 View
Hi, is hyperammonemia associated with worse outcomes of acute myocaridal infarction yet decreased risks of coronary and/or cerebrovascular events?
18 July 2022 3,718 5 View
Let me know, if someone have free sources (scripts) to run the analysis using R or MATLAB. Therby you can send me (if it is private) in my email: [email protected]. Thanks
01 July 2022 3,755 0 View
I am doing pathway enrichment analysis, the significant level is expressed by "-log(p-value)". What is it, and how it is different from FDR?
27 June 2022 5,738 0 View
I am conducting complement-dependent cytotoxicity assay using an IgG1 complement activating antibody and AML cells. The assay is working fine with rabbit complement, however, with normal human...
24 May 2022 9,184 0 View
I have done the extraction of chitosan from the shells of the shrimps, in the last step the deacytilization I used NaOH 50%, I have recovered the mass of chitosan ,filtered it and when I added...
11 May 2022 7,755 1 View
According to the literature review published in April 2022 by Walters et al (1) in the Cochrane Library, repeated doses of prenatal corticosteroids promote lung maturation in the baby and reduce...
08 May 2022 3,494 0 View