Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
We are using the Endogro-SCME004 medium for cultivation of hCMEC/D3 cells, but since it is out of stock for a while, we decided to switch to Lonza. However, we just realized that there are stark...
22 June 2023 5,062 0 View
Population, Sampling Size and Techniques
27 May 2023 9,634 6 View
it is a question of solving an equation by the method of the elements. I was able to find the solution on pdeMatlab. but the one if does not coincide with the one generated by my script.
22 May 2023 7,990 1 View
I'm trying to do transient transfection on snu638 cell line. I'm using flag tag plasmid for transfection , initially it got worked. But if I try to repeat it it is not working I tried different...
12 April 2023 9,298 0 View
please help me! I'm staining splenocytes with mitotracker deep red. Is it possible that I get some unstained cells also in the stained sample?
07 April 2023 8,457 0 View
Why mRNA and protein levels do not correlate?
06 April 2023 6,472 3 View
I want to extract plasmid DNA by Alkaline lysis method
05 April 2023 786 2 View
Quantum physics is the study of matter and energy at its most fundamental level. A central tenet of quantum physics is that energy comes in indivisible packets called quanta. Quanta behave very...
11 March 2023 6,037 7 View
I transfected 293T cells with a flag tagged protein (40kDa). After performing the westernblot and Flag antibody treatment I was supposed to see a band on 41 kDa. Instead,that I see clear band on...
06 March 2023 5,612 3 View
In B.subtilis spore surface display cot partner with native promoter is fused in frame with a passenger protein via a linker like GGGGS. The assumption here is that a fusion mRNA is formed, and...
01 March 2023 2,854 1 View
Sex in hard ticks (Ixodidae) is very easy to determine, but does anyone have a reliable method to determine the sex in soft ticks (Argasidae)? Thanks in advance. Manuel
17 February 2023 5,831 2 View
Physics incests billions in theory development and experiments to the problem of quantum theory-GR (general relativity) unification. Some hypotheses like holographic principle emerged born out of...
02 January 2023 5,882 4 View
I would like to get a script written in biopython that creates a list of genome accession numbers for the genus Pseudomonas. But I don't know enough about biopython to achieve this. The goal is to...
29 December 2022 4,395 5 View
Classical physics has always distinguished particles from waves. However, at the beginning of the 20th century, the newly discovered phenomena of atomic and subatomic particles as well as the...
23 December 2022 9,376 6 View
I am working with Pyromark Q24 protocol on bisulfite converted DNA that has been through a PCR and verified by gel. I have a very high substrate peak (~200-250) that is resulting in low nucleotide...
19 December 2022 8,712 1 View
I am doing fourplex mqPCR panel for pathogens by Taqman probe . I did well choose for different dyes option (reporting dyes). real time PCR is five channel 7500 applied biosystem. For primary...
12 December 2022 810 4 View
The abstract is as follows: Title: Quality of life of Iranian adults with neurofibromatosis 1: validity and reliability study Abstract Aim: Evaluate the quality of life of Iranian patients with...
29 November 2022 7,145 2 View
Does anyone have experience working with Total RNA spike - ins for total RNA extraction & miRNA quantification with rt - qPCR? I have been made aware of the c.elegans spike in however i would...
22 November 2022 9,181 0 View
I need a detailed explanation of haploview use to study haplotypes association with the outcome. I have read many resources, but did not manage to change my excel data of genotypes into ped and...
05 October 2022 2,200 0 View
Hello i have been calculate some parameters of ROH in the summary List, like percentage, mean and count in Plink,...
20 September 2022 1,159 1 View
I transduced my B cells with GFP containing plasmids using lentivirus and achieved 60% positivity. However, when I checked my cells under the confocal microscope, I can not see any GFP signals....
13 September 2022 9,050 4 View
I know that miRNAs are stable up to 3 to 5 years in plasma/serum but I want to include CSF samples to my study. The problem is that these samples are difficult to come by. I already have some in...
20 August 2022 2,566 0 View
I have been performing qPCR but i am getting zigzag pattern for my amplification plot. What could be the reason?
18 August 2022 3,748 0 View
We want to prepare sophorose in our laboratory and we have beta-glucosidase enzyme. We want to use sophorose in a liquid culture media for the increment of enzyme production.
07 August 2022 5,158 0 View