Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
03 February 2021 8,986 2 View
Hi all, hope you will be able to help me. My protocol requires me to use 100-200 fmole amplicon DNA (let's assume I need 200 fmoles). The amplicon DNA concentration I have is 5.54 ng/ul and the...
21 January 2021 8,333 2 View
I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...
17 January 2021 3,921 4 View
I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...
10 January 2021 2,454 3 View
Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...
01 January 2021 7,568 5 View
I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...
27 December 2020 10,067 2 View
Hello, I am doing SNP analysis for my gene. I have calculated SIFT and Polyphen score but I cannot find way to calculate REVEL score. Can someone guide me, how can I get this score? Thank you.
12 December 2020 2,221 7 View
I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...
08 December 2020 7,818 3 View
I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...
07 December 2020 8,599 3 View
Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...
07 December 2020 1,869 3 View
For example, can we use the microRNAs involved in specific stress in a plant submitted on mirbase.org as a source for analysis of those microRNAs in other stresses? Thank you in advance for your...
01 December 2020 5,416 3 View
Performing pulsed Doppler ultrasound on a single thyroid artery (upper or lower; right or left) is it sufficient for the study of the mean sytolic peak in order to differentiate Graves' disease...
01 December 2020 4,139 3 View
I'm looking at wild horse populations and trying to map their relations. Ultimately I'd like to assess their relatedness and inbreeding coefficients. However, the most important component right...
30 November 2020 5,406 3 View
I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...
27 November 2020 9,597 5 View
I have no prior knowledge of microRNAs and a member of my lab just started her project working on it. ive done primer designing before for my genes but it cant be applied to designing primers for...
24 November 2020 3,053 4 View
For example for an interaction study between a protein target.
23 November 2020 4,105 4 View
Hi! I would like to know what tools I could use to analyze interactions between a new lncRNA and possible protein/miRNA targets. The biggest problem I had to find such tools is that the RNA we...
21 November 2020 8,482 3 View
Hello everyone, I'm looking for miRNA expression level data (as measured by RNA-seq) in a specific tissue, but all papers I have consulted (and their relative supplemental material) do not report...
15 November 2020 9,877 2 View
Suppose we have given miRNA (that according to in silico analysis should bind to a specific sequence in RNA) that is labeled with a fluorescent dye (or is labeled otherwise) and we have isolated...
13 November 2020 5,303 1 View
I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...
12 November 2020 5,480 1 View
I am going to buy GoTaq® 2-Step RT-qPCR System(Promega). This kit uses oligo(dT)15 primer for first-strand cDNA synthesis. I have to design primers for qPCR for 4 mature microRNAs(miR-21, miR-155,...
10 November 2020 9,348 3 View
Electrochemical microrna biosensor
02 November 2020 5,369 1 View
Please suggest portals to retrieve normalized-quantile expressions of micro-RNAs (in RPM).My aim is to construct a correlation plot between the gene of interest and microRNA. I searched in human...
29 October 2020 6,511 3 View
Hello! I'm new to bioinformatics and cancer databases. I was exploring cbioportal and analyzing coexpression of different genes through scatter plots. I noticed that the axis are labeled as " RSEM...
22 October 2020 7,401 2 View