New network testing models for deep learning in biological testing ?

We tested four models: logistic regression (LogReg), multilayer perceptron (MLP), graph convolutional network (GCN), and edge-attention graph convolutional network (EAGCN). Are there other models...

08 November 2020 2,347 4 View

Where to find cancer datasets composed by clinical markers and gene expression levels?

Currently, I am validating a new machine learning approach. But, I need to find different cancer datasets composed by clinical markers and gene expressions. For now, a just found MMRF data set...

11 October 2020 9,012 2 View

Can I use a Glass electrode (standard used for pH measurement) for measuring ORP?

I have made a test to investigated: I measured the potential in mV of a standard +220mV solution using my pHmeter which gives a mV value with the pH. The result was +9mV. But this was the result...

06 October 2020 7,220 3 View

Does anyone have a Bragg Grating simulation example in comsol?

I was searching for a bragg Grating simulation example in comsol, but I did not find any. The only one that I found was incomplete. Does anyone have a Bragg Grating simulation example in comsol?...

03 October 2020 4,337 6 View

How to do validation on a new instrument?

Halloo scientists, please help me the procedure s on doing validation on new bought instrument/equipment before starting doing analysis

07 September 2020 1,590 3 View

Can my RNA interference have any influence in my endougenos control gene?

Good night all. I'm using RNAi to knockdown a transcription factor from a primary cell and I need to validate by westernblot. With Ponceau marking i can see that i'm putting the same amount of...

25 August 2020 263 3 View

Is there any method for tuning Subspace Predictive Controllers (SPC)?

My current research is about SPC and I'm interested in tuning strategies for these types of predictive control approaches. The cost function is on the photo and the tuning parameters are Np...

23 July 2020 1,176 1 View

How can I hide nodes with posterior probability under 0.5 in BEAST phylogenetic tree visualization?

06 July 2020 1,376 6 View

How do I stored nano particles in my workplace and how long?

I am looking for a technique which is used to store the nano particles without any property change.

23 June 2020 2,473 15 View

List of environmental journals with no publishing fees?

I'm looking for a well-indexed Environmental/ocean science journal to publish my research papers without any publication fee, could you help me?

20 May 2020 2,233 14 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View