Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...
21 October 2020 2,448 5 View
We have been assessing miR-155 in RNA recovered from FFPE DLBCL samples. We found that using the TaqMan® standard Taqman miR kit, which require a separate RT reaction for every miR to be studied,...
19 October 2020 10,016 3 View
I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...
16 October 2020 7,731 4 View
Hello to all. I am performing a thesis on Cognitive disorder modeling in rats and the effect of Pharmacological Treatment on the disease and the expression of certain miRNAs. I am seeking...
15 October 2020 6,784 1 View
Dear researchers, I have ordered customized primers for ticks candidate miRNAs as these miRNAs are not available in any company. I have used a miRNA-specific MystiCq cDNA synthesis kit for cDNA...
15 October 2020 5,725 7 View
Because i isolate 5 ml of culture media without serum and i got 1.2 ng miRNA . And i need 70 ng minimum that we can do PCR
29 September 2020 5,985 4 View
I designed Primers for miRNAs using mirPrimer algorithm. However when l analysed them by Oligoanalyzer 3.1, it shows Primer-dimer and secondary structure formation. I request your suggestion...
20 September 2020 4,295 3 View
I am using QIAGEN's AllPrep DNA/RNA/miRNA Universal Kit. The handbook doesn’t mention the possibility of additional DNase digestion post-elution, i.e. directly in the RNA solution. Can this...
17 September 2020 7,207 3 View
Dear researchers, I am facing some issues with Sybre green qPCR results. I have isolated total RNA from different organs of ticks using the Trizol RNA isolation kit. After cDNA preparation with a...
15 September 2020 5,196 5 View
For a novel circRNA identified, is there a web tool that can predict the miRNA (from miRBase) binding sites contained in this circRNA? Most tools I found can only input circRNAs from...
02 September 2020 3,066 4 View
I have extracted RNA from serum samples (using Qiagen miRNeasy serum/plasma), with the goal of performing qRT-PCR and/or RNA-seq to determine differential expression of miRNA in the serum samples....
31 August 2020 3,118 3 View
I have a very basic question as mentioned in the question when RNAseq technology measures the mRNA abundance if it is acquired after degradation of dsRNA by miRNA or before? I would like to...
26 August 2020 5,153 4 View
Different miRNA target prediction algorithms use different scores which is not comparable directly. So what is the most reliable way to get the consensus of results produced by different miRNA...
25 August 2020 9,780 4 View
RNA22, miRanda, TargetFinder, and psRNATarget are four different algorithms aiming at finding miRNA targets. Many research-work claims at using a consensus of the result produced by these...
24 August 2020 5,198 5 View
I want to know if a sequence is related to a pseudo microRNA precursor or not. I tried to use the Mipred database but it seems to be out of reach. I'm eager to know if there are any other...
21 August 2020 647 2 View
Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...
20 August 2020 5,290 7 View
Can I perform ceRNA network analysis when I have only lncRNA and mRNA data? My RNA-seq data only contains lncRNA and mRNA expression profile; I see many package support ceRNA analysis but require...
19 August 2020 966 6 View
Exosome isolation was performed from serum (Exosome precipitation reagent, İnvitrogen), and then when RNA was isolated from exosomes with a total RNA isolation kit(Magmax, miRVana) and measured...
18 August 2020 5,703 3 View
Hello, I'm at the stage of optimizing my qRT-PCR protocol. Can anyone tell me why my plot looks like this? I'm using Taqman advanced microRNA assay and universal PCR mastermix no amperase...
15 August 2020 5,683 3 View
On the basis of in-silico analysis we hypothesized that a long non-coding (lncRNAs) interacts with miRNA thus inhibiting that miRNA function in bread wheat. Now I want to isolate this lncRNA-miRNA...
13 August 2020 7,689 2 View
I have tested mirDB and target scan, but they seem to be predicted.
12 August 2020 6,471 3 View
I wanna do miRNA profiling in serum and plasma using SYBR green and I wanna know the best and cheap kit to extract miRNA. Which way is better: Extract miRNA directly using available kits followed...
06 August 2020 5,328 6 View
I have been running a small test experiment, extracting RNA from FFPE DLBCL blocks, and analysing miRs in the samples. So far I have been using the TaqMan™ MicroRNA Assay, which require a...
05 August 2020 1,311 1 View
Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...
31 July 2020 9,832 3 View