Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...
06 June 2019 2,467 10 View
I am asking for help in identifying known miRNAs in plants. After bioinformatic analyzes, I have a list of miRNAs identified and I do not know what kind of criteria adopt in order to find among...
06 June 2019 869 11 View
I forgot to put my taqman miRNA assay(thermofisher) at -20degree. It was at 4degree for 48hours. Will it be working?
06 June 2019 6,571 6 View
I have prepared miRNA cDNA using Taqman advanced miRNA cDNA synthesis kit and used them with Taqman qPCR Assay. Now i want to assess expression level of more miRNAs in same samples using SYBR...
06 June 2019 6,917 6 View
We are trying to find the biological correlation between miRNA and certain genes. We found the general correlation through IPA (Ingenuity Pathway Analysis). Thank you for your inputs!
06 June 2019 6,985 6 View
I transfected miR-301a inhibitor in a cell line @ 20nM-40nM using miRVANA inhbitor. In qPCR of miR-301a expression showed 2-3 times fold difference comparing to the control (scr tranfection)....
06 June 2019 802 4 View
Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...
06 June 2019 6,017 7 View
Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.
06 June 2019 9,814 3 View
Since I'm working with circular RNA, I routinely want to check a custom RNA sequence (not a standard 3' UTR) for binding sites for miRNA. I want to check a given sequence against all known human...
06 June 2019 3,597 3 View
I'm looking for a tool that predicts potential miRNA targets. I can find several tools that predict this for WT sequences (DIANA tools and others let you introduce gene ID for this), but I'm...
06 June 2019 241 4 View
I have previously used U6snRNA in case of cell specific miRNA quantification. This time in case of plasma I am using this and getting the relevant CT value.I was just wondering if U6 is a reliable...
06 June 2019 715 4 View
06 June 2019 8,878 13 View
I wanna see the expression of human micro rna let 7c in HPV positive cervical swab sample. But i couldn't find any validated primer sequence of this micro rna. If anyone have any idea regarding...
29 May 2019 2,320 1 View
I want to evaluate the ionizing radiation effects on miRNA expression level in mice. Some paper used PBMCs and others used whole blood ( WBC ) for RNA extraction . is there difference between...
26 May 2019 4,902 4 View
Hey everybody, I would like to isolate exosomes out of plasma. I‘m wondering if somebody has used the ExoQuick (BIC) or the Total exosome isolation reagent (from plasma, ThermoFisher) and can...
20 May 2019 279 3 View
hi guys suggest me the method or database from where I can download all the target genes present in Drosophila for it's each miRNA.
17 May 2019 7,798 3 View
I am a ph.D student. I am going to work on some microRNAs related to breast cancer. I would like to design an interaction between selected the microRNAs and their targets
05 May 2019 4,138 2 View
05 May 2019 7,728 1 View
05 May 2019 8,085 5 View
Suggest me database that will provide the sequence of target gene of miRNA in Drosophila melanogaster
05 May 2019 3,861 1 View
I'm trying to use circRNA Viewer to build a network of interaction with miRNA. But right now I wasn't able to do anything except load the database that came with the program, have you got any...
05 May 2019 5,608 1 View
05 May 2019 2,398 4 View
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
22 April 2019 350 4 View
I am trying to overexpress a target gene in bovine adipocytes, i have clonned my target gene to pcDNA3.1 vector. There is 3 fold increase in the expression of target gene in the cells transfected...
10 April 2019 3,855 4 View