Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I have used iTRAQ for analysis of the arabidopsis secretome, however, I am now hoping to apply this method to the whole proteome of various crop species. Advice on sample preparation or...
01 January 2020 6,222 3 View
Can anyone help me to identify miRNAs from Assembled Transcriptome (multi fasta contig files around 45,000). I checked with mirBase but it has some limitations. I did a local BLAST using mature...
01 January 2020 8,342 3 View
I am going to collect venous blood samples for the thesis project. and my inquiry is, does fixed-angle centrifuge suitable to be used for plasma fractioning for miRNA extraction? especially, K2...
01 January 2020 2,077 2 View
Potential of miRNA and svRNA-N antagomirs as antivirals Missed in Chinese (ethnicity) This is engineered virus, Will stop after 35 days. Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1,...
01 January 2020 6,432 2 View
For miRNA delivery studies if conjugating and loading is required what temperatures and pH will it be stable at and what conditions should be avoided to prevent its degradation?
12 December 2019 7,808 6 View
I wnat to develop electrochemical biosensor to detect miRNA. I have probe miRNA Target miRNA single mimatch mirna
12 December 2019 6,530 1 View
articles showed that blocking lncRNA causes increase in miRNA detected in reat-time PCR
12 December 2019 6,244 2 View
why the RNA is very instable and because of its OH group is very degradable and the lncRNA is very stable and can inhibit or active the gene for rest of the life?
12 December 2019 8,738 0 View
I am trying to isolate pancreatic islets by infusing 1mg/ml collagenase solution but digestion was not perfect. Any suggestion?
03 December 2019 4,013 1 View
Hi all, I am producing AAV vector-containing shRNA to knockdown my target protein. Three pDNAs (transfer plasmid, pHelper, RepCap) are transfected into HEK293A (80% confluency). I can get a very...
28 November 2019 2,319 6 View
Hi! It is very similar to Nanodrop. It worked very well, however, the display doesn't turn on today. Do you know this equipament? Anyone can help me, please? Thanks!
21 November 2019 4,015 3 View
Have heard there is alot of contamination via TRIZOLE and may affect results therefore i want to extract miRNA from tissues and plasma..For Plasma i am using miRNA PAris VAna Kit but for tissues i...
20 November 2019 7,268 2 View
We have a bag of filter tips in our lab from some time ago and we are not sure about its sterility and how it could have been manipulated before. Since I have to do ARN extraction and qPCR, I want...
12 November 2019 4,839 2 View
11 November 2019 886 3 View
Hi everyone! I want to study some microRNAs expression in autoimmune diseases such as Hashimoto and graves diseases. I would like to help me with how can I select them? Is a certain database or...
11 November 2019 2,047 1 View
Hi, I am interested in understanding the interaction between a specific miRNA and a polyphenol. Are there any in silico approaches that I could use ?
11 November 2019 3,187 3 View
Plasma is required for miRNA analysis but in our settings we have to take sample from long distant place sometime from outskirts so that the sample(plasma) seperated cant be stored straight away...
11 November 2019 9,408 3 View
Hello there. I will try to transient transfect the U-2 OS cell line (an osteosarcoma cell line) with miRNA mimics using Lipofectamine RNAiMAX in a 6-well plate format. Does anyone have any...
11 November 2019 3,049 3 View
I want to analyse text file of miRNA in following format and identify differentially expressed miRNA. How to do this using any available tools?
11 November 2019 7,849 3 View
10 November 2019 2,217 1 View
09 November 2019 1,223 3 View
06 November 2019 9,485 3 View
05 November 2019 1,604 3 View
I am looking to understand the mechanisms by which siRNA works to inhibit RNA production versus microRNA does anyone know the average time these molecules stay active in the body? My preconception...
24 October 2019 4,465 3 View