Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I obtained mouse serum samples. My initial plan is to check some proteins using ELISA. However, plans have changed and now I need to check some gene expressions. My questions are: 1) Can I...
09 June 2021 866 3 View
any mechanism indicating the role of storage proteins in salt stress tolerance?
01 June 2021 4,463 3 View
Hi All, I am doing a research on Regression Test Cases Prioritization with Neuro Fuzzy System. Is there any suggestions How can I implement this on MATLAB or any other Platform? Thanks
31 May 2021 1,629 2 View
I am seeking to isolate miRNA from serum using Qiagen serum/plasma advanced kit - after running the smRNA bioanalyzer QC on the RNA only 60mer and higher are represented with no 20mer. Why is this...
28 May 2021 5,822 1 View
I want to visualize EOMES, a transcription factor, in CD8 T cells. I am planning to perform an immunofluorescence test and I was wondering what kind of stain or chemicals I can use to enable the...
18 May 2021 9,940 7 View
I tried to synthesize samarium nanoparticles using curcumin. I used samarium nitrate as a processor and curcumin as a reducing agent. I put the solution of samarium nitrate and curcumin on a...
18 May 2021 3,887 1 View
I have been trying to reduce the background signal in southern blot. I always get a dark stain (not resembling bands) in random places of my membrane. I do the hybridisation step in a...
11 May 2021 7,846 0 View
We are planning a study to assess awareness and attitude regarding genetic testing among general population.
07 May 2021 5,073 2 View
Usually, overexpression of a gene rescues the cells from the phenotypes it gives due to ablation of the same gene.
01 May 2021 3,597 2 View
Hey everyone, I am trying to do a miRNA-Seq experiment. I have prepared the libraries and before sequencing, I did QC measurements on the Agilent Bioanalyzer 2100, HS DNA Kit. I have some weird...
13 April 2021 8,217 1 View
Dear all, I have a set of genes retrieved from mass spec data, where I have the level of abundance and phosphorylation expressed in Log2FC. I would like to sign up and downregulated genes and...
13 April 2021 686 1 View
Hello everyone and thanks in advance for taking the time to read through my question! I am doing some Seahorse experiments on macrophages (not an immortalized cell line, in case that it makes any...
13 April 2021 4,179 0 View
Hi there! we are trying to measure the concentration of miRNA, after using miRNA extraction plasm/serum kit (Qiagen), by nano drop but the concentration is very low, so any one has an experience...
21 March 2021 9,094 5 View
I have mouse testicular cells, from which I isolate spermatogonia (non-stem) using MACS. The buffer I use in MACS is 1xPBS supplemented with 0.5% BSA (bovine serum albumin) and 2mM EDTA. After...
10 March 2021 2,282 1 View
I want to know about the advantages of TCEP (tris(2-carboxyethyl)phosphine) over other reducing agents such as dithiothreitol (DTT) and β-mercaptoethanol.
09 March 2021 1,428 6 View
How would expression of a gene targeted by thyroid hormone be affected by deletion of thyroid hormone receptor in the absence of hormone? How about in thyroid hormone-treated cells? Please,...
07 March 2021 2,440 4 View
Myasthenia Gravis is a potentially fatal auto-immune disease. Its symptoms (progressive and then recovering muscle fatigue) and their cause are well understood. The adaptive immune system goes...
04 March 2021 6,958 9 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
25 February 2021 7,540 3 View
Hi, I want to extract DNA from ticks (to detect Haemoparasite like Babesia, Theileria, ). What is the best method to crush ticks? Which kit gives a better result for isolation and identification...
12 February 2021 2,994 4 View
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
03 February 2021 9,181 2 View
Hi all, hope you will be able to help me. My protocol requires me to use 100-200 fmole amplicon DNA (let's assume I need 200 fmoles). The amplicon DNA concentration I have is 5.54 ng/ul and the...
21 January 2021 8,371 2 View
I am looking for a webbased mouse lncRNA database for function predictions. All the websites i found are human based
21 January 2021 738 0 View
I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...
17 January 2021 4,054 4 View
I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...
10 January 2021 2,575 3 View