How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Should I test for HWE in dataset of inbred animals without random mating?

Hi, I am doing a GWAS analysis of european bison's a dog's SNPs. Right now I am doing multiple data clean up, according to previous studies and what I have learned. I was thinking, if it is ok to...

18 March 2020 3,103 3 View

How to insert Cre into an outbred strain?

I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...

02 February 2020 4,847 4 View

How to insert Cre into an outbred strain?

I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...

01 February 2020 5,895 4 View

Liometopum microcephalum mating behavior?

22 June 2019 1,545 3 View

Liometopum microcephalum mating behavior?

05 June 2019 3,191 4 View

My donor and recipint parents are showing amplification with same specific primers. please suggest why?

I am working on marker assisted backcross breeding in rice and during the foreground selection I can see the band in donor as well as recipient parent although it is of different band size. The...

24 April 2019 6,946 3 View

My donor and recipint parents are showing amplification with same specific primers. please suggest why?

I am working on marker assisted backcross breeding in rice and during the foreground selection I can see the band in donor as well as recipient parent although it is of different band size. The...

03 April 2019 713 3 View

Please could anyone guide me on how to edit (width/color/lines etc) a pedigree using kinship2 in R?

I am working with an extended family, I already have the pedigree but I want to edit the figure because it is huge, so the lines are very thin and the squares and circles are very small. I thought...

01 February 2019 4,829 3 View

What is role of "Genetic Counselor" in ophthalmology clinics?

Genetics seems to have generated immense knowledge about various eye diseases. These findings include gene mutations, their effects, pattern of inheritancel etc. So, Is this the time to have...

04 May 2018 2,127 10 View

How to design primers for validation of alleles in RIL population ?

Hello dears I want to design primers for validation of alleles in RIL population of two inbreed lines one is sensitive and second is resistance. I have run the PCR of two lines and got the...

01 February 2018 6,119 2 View