A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached.... | Contact experts in RNA to get answers
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Questions related to RNA
I will do a GSIS in min6 cells and normalize the results to total protein, after taking the supernatant for high glucose stimulation do I need to wash the cell with PBS before adding lysis buffer...
03 October 2022 2,931 3 View
I have to extract ARN viral from aerosoles, this aerosoles are grown in agar. I tried to solve this with ultrapure water and temperature, but I don't have good results in PCR. Somebody can help me?
27 September 2022 6,352 0 View
I am working with ligand conjugation with proteins. I want to conjugate my protein with ligand via cysteine residue. I have computed the surface area accessibility score of all cysteines. How can...
21 September 2022 8,152 1 View
I've been having a bit of trouble with yeast contamination for DU145 cells. I purchased nystatin to rid the contam but nystatin will not be able to help with any potential bacterial contam. Is it...
12 September 2022 1,542 5 View
There are incidences of Falciparum malaria cases in my working place who have been de-inducted recently from South Sudan. According to the patients they were using Mefloquine prophylaxis weekly ,...
09 September 2022 7,451 4 View
Hello, Currently, I am conducting an experiment using a reporter cell (a mammalian cell expressing target protein in cytosol). The experiment is not going well. So I would like to check whether...
07 September 2022 4,397 3 View
Hi Folks, I'm looking for in-silico methods or tools to assess the effect of mutations on bacterial transcription factors and membrane proteins. What approach (methodology) can someone take while...
07 September 2022 5,205 2 View
I performed RNA isolation from B. subtilis (lysozyme combined with Trizol reagent method) and RNA samples were treated with DNase I. I had samples for running an agorose gel both before and after...
02 September 2022 786 1 View
I did DNA extraction using Qiagen DNeasy Plant Mini Kit. I ran out of RNaseA and I did not add RNaseA in couple of samples. Nanodrop reading is very different for samples with and without...
02 September 2022 1,831 3 View
I want to diagnose my mutant plants. So I am looking for an easy method for diagnosing the chromosome.
29 August 2022 8,769 0 View
I am working with Drosophila embryos and I want to sort GFP positive nuclei by FACS, to analyze them by bulk RNA-seq. To nuclei extraction I am following Fly cell atlas protocol. Afterwards I sort...
25 August 2022 7,052 0 View
I'm trying to establish a bovine knock-in cell line using CRISPR-Cas9. I designed a donor DNA containing the transgene, a GFP gene and the homology arms. Should the donor DNA have a antibiotic...
25 August 2022 4,611 1 View
I am trying to see if DNA purification step will increase nematode detection in soil DNA. Soil DNA was extracted and half of the volume was used as it is (raw DNA extract) whereas remaining half...
24 August 2022 5,847 2 View
hi, I am performing DNA extraction from cervical swab using QIAGEN QIAAMP DNA MINI KIT and I am incubating my samples at 56c for 1 hour but the thing I am noticing is that I am not getting enough...
20 August 2022 4,100 1 View
I am doing total RNA extraction from PAXgene blood RNA tubes (6.9 ml of storage buffer + 2.5ml of collected blood in each tube) using the PAXgene blood RNA kit. I just want to extract the total...
16 August 2022 4,832 1 View
Chloroform saturated PBS
16 August 2022 6,298 0 View
Does anyone know any online open source or free sites where I can search or check on gene variant classification eg. ACMG (Benign , pathogenic, etc) besides ClinVar? Thanks
05 August 2022 7,760 0 View
Hello every one We've done an experiment on As toxicity in 2 different tissues. we've evaluated oxidative stress, biochemical and other parameters. Now, we want to know, in which tissue, As has...
01 August 2022 9,524 0 View
Microbiome is full collection of genes of all the microbes in a community. The Microbiome consists of microbes that are both helpful and potentially harmful. How does this microbiome effects the...
31 July 2022 259 1 View
I have Autodock 4.2.
22 July 2022 4,571 2 View
Dear all, I was wondering if someone has advise for me in the following: I am trying to extract from stomach content (degraded DNA, lots of blood/non-target tissue in the sample) with the Qiagen...
19 July 2022 1,071 0 View
I am isolating and culturing the monocytes derived from patient blood samples. The following step followed- 1. Blood + PBS (1:1) mixed and topped up on the histopaque layer. Centrifuged at 400 g,...
13 July 2022 5,696 7 View
Hi everybody, I am extracting DNA from FFPE samples. This samples will be used to measure copy number variation in several cases of neuroblastoma through Oncoscan technology. Due to a mistake, I...
12 July 2022 8,772 0 View
Hi there. I have some data from RNA-seq experiments. In PCA, we observe that the dominating patterns in our data are explained by other variables, rather than by those we used to classify our...
12 July 2022 3,530 1 View