Identification of long noncoding RNA Promoter for CRISPRa?

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Does there exist any empirical evidence of government expenditure financed by money collected through lobbying?

Basically, i am trying to understand from the literature whether lobbying can also considered an alternative source of financing fiscal deficit.

26 July 2024 8,692 5 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

RNA isolation from plant roots infected with both bacteria and fungi. How to isolate RNA from all of them simultaneously?

I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...

09 June 2024 9,876 0 View

How can I find capacity of Li-Ion coin cell (CR2023) battery ?

if I want to run my battery cycling test in 1 C rate ,but then I need to know the capacity of my material used in the coin cell (i.e. if my capacity is 20mAh then I will put a constant current of...

05 May 2024 1,364 1 View

Are there any suggestions or insights you can provide to help address this convergence failure in my optimization process?

I'm currently experiencing convergence failure while attempting to optimize a dimeric liquid crystal molecule under the influence of an electric field. The optimization code I'm using is...

10 March 2024 4,737 0 View

Does the dipole moment of a molecule correlate with its hyperpolarizability and polarizability?

implying that a molecule with a lower dipole moment exhibits lower hyperpolarizability and polarizability compared to one with a higher dipole moment?

07 March 2024 572 2 View

What is the script for running protein cluster by using DBSCAN?

Im trying to run dbscan.py by using vmd (dcd and pdb) files but the script is showing error. Its not generating cluster it's only generating noise from the trajectory file. How to solve this issue...

03 March 2024 6,414 0 View

How can I effectively analyze my data using multinomial logistic regression in SPSS?

I am conducting a study that involves analyzing categorical outcome variables with multiple levels and want to employ multinomial logistic regression analysis in SPSS for my data. However, I'm...

29 February 2024 8,533 4 View

Why is my IS high in plasma when measured in GCMS?

Hello everyone, I am currently working on developing a method to quantify butyric acid in plasma on single quad-GCMS using electron impact mode. For this purpose, I am currently following this...

14 February 2024 3,919 0 View

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes?

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?

29 January 2024 3,222 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Mehrdad Mohebi Shahpour Abadi

Dear Sankalpa Chakraborty,

I hope these articles are useful.

https://blog.addgene.org/how-to-design-your-grna-for-crispr-genome-editing

Article Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

Article CRISPRi-based genome-scale identification of functional long...

Article Functional Classification and Experimental Dissection of Lon...

Article When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells

Best Wishes

Sankalpa Chakraborty

Thank you.