I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label.
I need the fluorescent label that will not affect the length of my miRNA.
I found some kit such as ULYSIS Nucleic acid labelling kit, Label IT, miRVANA, and N Code.
But in many paper, they usually used for microarray. Can they be used in my project?
Can you get the RNA made with any of the fluorescein or ABI fluorescein dyes..Fam,Tet etc already attached to either the 5' end or internal base position?
Paul Rutland Hello Sir. Paul, thankyou very much for the suggestion. So.. from what I get, you suggested that I order the synthetic target miRNA that has already been labelled from company?
Yes Arnawati Jo getting it made guarantees that every molecule has a label and each molecule has only one label. Contact whichever company makes your dna primers to discuss prices and which label to incorporate. It depends a bit on how much rna you need as huge amounts may be expensive but oligo companies almost all make rna and it will most likely be PAGE or column purified and your institute may get price discount
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