Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I transduced my B cells with GFP containing plasmids using lentivirus and achieved 60% positivity. However, when I checked my cells under the confocal microscope, I can not see any GFP signals....
13 September 2022 9,041 4 View
I know that miRNAs are stable up to 3 to 5 years in plasma/serum but I want to include CSF samples to my study. The problem is that these samples are difficult to come by. I already have some in...
20 August 2022 2,554 0 View
I have been performing qPCR but i am getting zigzag pattern for my amplification plot. What could be the reason?
18 August 2022 3,739 0 View
We want to prepare sophorose in our laboratory and we have beta-glucosidase enzyme. We want to use sophorose in a liquid culture media for the increment of enzyme production.
07 August 2022 5,150 0 View
Hello, Recently I’ve been working on periplasmic expression of his-tag fusion protein. I’ve been struggling with 2 different manual purification that I’ve been found. Which protocol is better to...
19 July 2022 7,725 6 View
I am doing pathway enrichment analysis, the significant level is expressed by "-log(p-value)". What is it, and how it is different from FDR?
27 June 2022 5,643 0 View
Hi, Our wet-lab results inhibit bacterial growth against certain compounds. We are interested to predict its possible targets from bacteria. I came to know about Inverse docking but I did not find...
20 April 2022 6,536 2 View
Regarding genetic testing
29 October 2021 6,497 1 View
I am running and experiment where I use cryopreserved PBMCs as the following: 1) thawing and incubation on RPMI-1640 in CO2 incubator overnight 2) activation with IFN-alpha for 15 min 3) fixation...
15 October 2021 6,966 3 View
We have a problem with MIN6. I don't know certainly the number of passage that they are. The first passage that we have has an appropriate reaction on adding glucose, but the fourth does not...
13 October 2021 9,017 0 View
Hello. I am working on differential expression of 5 miRNAs in 3 different accessions of tomatoes named Solanum pimpinellifolium, Solanum lycopersicum, and Solanum habrochaites under abiotic...
10 October 2021 1,138 2 View
I am performing magnify chromatin immunoprecipitation on thalamic tissue samples to determine if the primers we have for VGAT work via q-PCR. Before I ask my question, I want to put out there...
26 September 2021 7,376 0 View
Hi, I am interested in a chromatin state over one of the promoters in different cell lines. I would like to check it by ChIP-qPCR. The question is how to show it? Is a one set of primers...
26 August 2021 2,554 1 View
Hello all, I am fairly new to the world of RNA and sncRNA, so I appreciate any input. I am currently trying to amplify miRNA sequences with primers that I generated using the miRprimer algorithm...
16 August 2021 1,308 2 View
I am performing miRNA loss-of-function study in cells and using a reporter assay to validate miRNA inhibition. So, I am planning on cloning the entire 3’-UTR sequence of our miRNA target...
09 August 2021 7,217 3 View
I am looking for host genes coding these lncRNAs and mRNAs.
18 July 2021 6,313 2 View
Human RNA extraction from serum: Can someone please help me with the protocol?
07 July 2021 5,588 6 View
hello everyone, I have a results for interaction between miRNA – lncRNAs and interaction between mRNA- lncRNA. I want to draw network in cytosape but faced error such as (Gene identifier Error,...
23 June 2021 7,922 0 View
I am seeking to isolate miRNA from serum using Qiagen serum/plasma advanced kit - after running the smRNA bioanalyzer QC on the RNA only 60mer and higher are represented with no 20mer. Why is this...
28 May 2021 5,773 1 View
I want to visualize EOMES, a transcription factor, in CD8 T cells. I am planning to perform an immunofluorescence test and I was wondering what kind of stain or chemicals I can use to enable the...
18 May 2021 9,877 7 View
Hey everyone, I am trying to do a miRNA-Seq experiment. I have prepared the libraries and before sequencing, I did QC measurements on the Agilent Bioanalyzer 2100, HS DNA Kit. I have some weird...
13 April 2021 8,134 1 View
Hi there! we are trying to measure the concentration of miRNA, after using miRNA extraction plasm/serum kit (Qiagen), by nano drop but the concentration is very low, so any one has an experience...
21 March 2021 9,030 5 View
I want to know about the advantages of TCEP (tris(2-carboxyethyl)phosphine) over other reducing agents such as dithiothreitol (DTT) and β-mercaptoethanol.
09 March 2021 1,384 6 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
25 February 2021 7,314 3 View