Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
Hello. I moved to a new lab. My IP solution, which I used at previous labs, stopped working. I can not hold a cell for more than 5-7 minutes, then leak current increases dramatically and I lose...
30 May 2024 2,240 4 View
If so do you have to crosslink the compound to the oligo? If you have done this kind of experiment I would really appreciate any guidance/protocols. Thanks, Katie
22 May 2024 277 1 View
I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? Targeted gene part is 18bp long But...
11 May 2024 3,064 4 View
Hi, My research will consist in examining the effect of compounds at concentrations of 10(-4)M, 10(-5)M, 10(-6)M, 10(-7)M, 10(-8)M on cells. I prepared 100 mM stocks of compounds in 100% DMSO....
22 April 2024 3,002 0 View
I am going to extract total RNA from rat brains for RNA-seq. Does anyone have any experience with using TriZol + RNeasy kit (Qiagen) or Direct-zol kit (Zymo)? Which method is better? Thanks
19 April 2024 9,781 0 View
I just want to know as there is pedagogical leadership and pedagogy is much explained, however the relationship between assessment of students' performance and teacher's employed pedagogy.
03 April 2024 1,566 7 View
Maybe someone knows why this happens. The situation is that after PCR purification of gel products (cut one band), on the next electrophoresis, instead of one band, two bands appeared, how could...
19 March 2024 4,223 2 View
Please can anyone help me that from where I can find the chain identifier where the mutation occurs in my protein. I need to enter the chain in DynaMut2 for my mutation.
05 February 2024 4,043 0 View
I am trying to use DynaMut2 to predict a change in protein TBX4 after a mutation. please can someone help me that from where I can take information about the chain of my protein? i need to enter...
05 February 2024 6,476 0 View
Stage Temp Time Temp/time 1 95°C 5min - 2 95°C-85°C -...
01 February 2024 1,888 0 View
I am currently doing researching on a novel technique to detect antibiotic resistance genes by doing multiplex realtime PCR melting curve analysis. It has been working so far, however, I am...
26 January 2024 9,844 0 View
We assume that this is absolutely true because they tried to introduce and adjust matrix mechanics only to quantum particles when it should be applied to both classical physics situations as well...
18 January 2024 7,561 4 View
Hello, The reviewer raised a question about our paper, stating that the "MTT assay (cytotoxic assay) is not equal to anti-cancer". However, I've come across papers with similar titles. Can you...
15 January 2024 7,107 7 View
Hi all, I'll be explaining the situation briefly. I'm working with two cancer cell lines: T24 and UMUC-3 cells. I'm trying to do a knockdown for the SLC38A2 gene using siRNA and Hiperfect...
19 December 2023 4,235 0 View
Hi, Would anyone know which generation of lentiviral vectors were used to generate hCMEC/d3 cell line? Thank you, Ecem
01 December 2023 7,459 2 View
I have read a research paper Ageratum Yellow Vein Virus isolated from a tomato plant with leaf curl on Ishigaki Island, Okinawa, Japan. According to this research, the paper confirmed that AYVV is...
06 November 2023 3,499 0 View
Safety of blood donations could be affected due to a donor's previous exposure to transfusion-transmissible infections by a number of various routes. A donor selection criteria must be established...
03 September 2023 3,069 1 View
Hi there, I am comparing the abundance of a protein in hypoxia (5% oxygen) versus normoxia (21% oxygen). I am assessing changes in my protein via western blot, and I used GAPDH as a loading...
30 August 2023 3,239 3 View
I did CHIP qPCR for transcription factors on a promoter, I got fold enrichments (compared to control IgG) as 2.54, 2.75, 3.45 and 21 for diffferent transciption factors in different sites. Can...
29 August 2023 4,744 0 View
Is anyone can help me in the determination of enzyme activity for papain and bromelain enzymes in a mixture ?
28 August 2023 3,527 3 View
Has anyone used yeast nitrogen base (YNB) to make broth media at alkaline pH of 9-10? I tried buffering with HEPES, Tris, and Glycine but precipitation occurred all the time when the pH increased...
17 August 2023 769 0 View
I am looking to mix immunoprecipitation reactions in a 96-well block and trying to find a rotator that can handle this requirement. Appreciate any insight beyond modifying an existing rotator.
14 August 2023 3,451 0 View
I am current doing with drug analysis and for my opium and heroin samples, they shows a deep green colour with Froehde reagent? The results are constant with many samples and in fact it should...
09 August 2023 7,122 0 View
Dear all, I'm having problems determining the proper erastin dose in the SH-SY5Y cell line. Erastin activates ferroptosis and results in ferroptotic cell death. I used a 96-well plate with 4000...
28 July 2023 4,752 1 View