Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
1,774 views 2,540 posts
Questions related to microRNA
We assume that this is absolutely true because they tried to introduce and adjust matrix mechanics only to quantum particles when it should be applied to both classical physics situations as well...
18 January 2024 7,665 4 View
Hello, The reviewer raised a question about our paper, stating that the "MTT assay (cytotoxic assay) is not equal to anti-cancer". However, I've come across papers with similar titles. Can you...
15 January 2024 7,195 7 View
Provide answers from meditations published by trinity college foundation
08 January 2024 8,874 0 View
Hi all, I'll be explaining the situation briefly. I'm working with two cancer cell lines: T24 and UMUC-3 cells. I'm trying to do a knockdown for the SLC38A2 gene using siRNA and Hiperfect...
19 December 2023 4,308 0 View
Hi, Would anyone know which generation of lentiviral vectors were used to generate hCMEC/d3 cell line? Thank you, Ecem
01 December 2023 7,538 2 View
Hi, I would like to immobilize an oligo onto a nitrocellulose membrane. Which way is the best? Is it possible to conjugate an oligo with covalent binding? Thanks in advance for your...
30 November 2023 6,300 0 View
I have read a research paper Ageratum Yellow Vein Virus isolated from a tomato plant with leaf curl on Ishigaki Island, Okinawa, Japan. According to this research, the paper confirmed that AYVV is...
06 November 2023 3,592 0 View
Safety of blood donations could be affected due to a donor's previous exposure to transfusion-transmissible infections by a number of various routes. A donor selection criteria must be established...
03 September 2023 3,168 1 View
Hi there, I am comparing the abundance of a protein in hypoxia (5% oxygen) versus normoxia (21% oxygen). I am assessing changes in my protein via western blot, and I used GAPDH as a loading...
30 August 2023 3,317 3 View
I did CHIP qPCR for transcription factors on a promoter, I got fold enrichments (compared to control IgG) as 2.54, 2.75, 3.45 and 21 for diffferent transciption factors in different sites. Can...
29 August 2023 4,846 0 View
Is anyone can help me in the determination of enzyme activity for papain and bromelain enzymes in a mixture ?
28 August 2023 3,591 3 View
Has anyone used yeast nitrogen base (YNB) to make broth media at alkaline pH of 9-10? I tried buffering with HEPES, Tris, and Glycine but precipitation occurred all the time when the pH increased...
17 August 2023 846 0 View
I am looking to mix immunoprecipitation reactions in a 96-well block and trying to find a rotator that can handle this requirement. Appreciate any insight beyond modifying an existing rotator.
14 August 2023 3,544 0 View
I am current doing with drug analysis and for my opium and heroin samples, they shows a deep green colour with Froehde reagent? The results are constant with many samples and in fact it should...
09 August 2023 7,187 0 View
Dear all, I'm having problems determining the proper erastin dose in the SH-SY5Y cell line. Erastin activates ferroptosis and results in ferroptotic cell death. I used a 96-well plate with 4000...
28 July 2023 4,818 1 View
We are using the Endogro-SCME004 medium for cultivation of hCMEC/D3 cells, but since it is out of stock for a while, we decided to switch to Lonza. However, we just realized that there are stark...
22 June 2023 5,140 0 View
Population, Sampling Size and Techniques
27 May 2023 9,735 6 View
it is a question of solving an equation by the method of the elements. I was able to find the solution on pdeMatlab. but the one if does not coincide with the one generated by my script.
22 May 2023 8,054 1 View
I'm trying to do transient transfection on snu638 cell line. I'm using flag tag plasmid for transfection , initially it got worked. But if I try to repeat it it is not working I tried different...
12 April 2023 9,339 0 View
please help me! I'm staining splenocytes with mitotracker deep red. Is it possible that I get some unstained cells also in the stained sample?
07 April 2023 8,516 0 View
Why mRNA and protein levels do not correlate?
06 April 2023 6,542 3 View
I want to extract plasmid DNA by Alkaline lysis method
05 April 2023 855 2 View
Quantum physics is the study of matter and energy at its most fundamental level. A central tenet of quantum physics is that energy comes in indivisible packets called quanta. Quanta behave very...
11 March 2023 6,136 7 View
I transfected 293T cells with a flag tagged protein (40kDa). After performing the westernblot and Flag antibody treatment I was supposed to see a band on 41 kDa. Instead,that I see clear band on...
06 March 2023 5,665 3 View