Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I am using QIAGEN's AllPrep DNA/RNA/miRNA Universal Kit. The handbook doesn’t mention the possibility of additional DNase digestion post-elution, i.e. directly in the RNA solution. Can this...
17 September 2020 7,227 3 View
I am trying to recreate a protocol that has PCR tubes with a total volume of 50 µL. Now in the paper some oligonucleotides are added in different molarities; Enrichment oligo 400 nM Coupling...
16 September 2020 2,283 3 View
Dear researchers, I am facing some issues with Sybre green qPCR results. I have isolated total RNA from different organs of ticks using the Trizol RNA isolation kit. After cDNA preparation with a...
15 September 2020 5,211 5 View
For a novel circRNA identified, is there a web tool that can predict the miRNA (from miRBase) binding sites contained in this circRNA? Most tools I found can only input circRNAs from...
02 September 2020 3,075 4 View
I have extracted RNA from serum samples (using Qiagen miRNeasy serum/plasma), with the goal of performing qRT-PCR and/or RNA-seq to determine differential expression of miRNA in the serum samples....
31 August 2020 3,125 3 View
I have a very basic question as mentioned in the question when RNAseq technology measures the mRNA abundance if it is acquired after degradation of dsRNA by miRNA or before? I would like to...
26 August 2020 5,176 4 View
Different miRNA target prediction algorithms use different scores which is not comparable directly. So what is the most reliable way to get the consensus of results produced by different miRNA...
25 August 2020 9,804 4 View
RNA22, miRanda, TargetFinder, and psRNATarget are four different algorithms aiming at finding miRNA targets. Many research-work claims at using a consensus of the result produced by these...
24 August 2020 5,223 5 View
I want to know if a sequence is related to a pseudo microRNA precursor or not. I tried to use the Mipred database but it seems to be out of reach. I'm eager to know if there are any other...
21 August 2020 676 2 View
Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...
20 August 2020 5,317 7 View
Can I perform ceRNA network analysis when I have only lncRNA and mRNA data? My RNA-seq data only contains lncRNA and mRNA expression profile; I see many package support ceRNA analysis but require...
19 August 2020 980 6 View
Exosome isolation was performed from serum (Exosome precipitation reagent, İnvitrogen), and then when RNA was isolated from exosomes with a total RNA isolation kit(Magmax, miRVana) and measured...
18 August 2020 5,727 3 View
Hello. I am performing the analysis of some lncRNAs, and many of them present their structure only in the NONCODE or RNAcentral database. When I try to open the genomic position of these lncRNAs...
18 August 2020 7,594 0 View
Hello, I'm at the stage of optimizing my qRT-PCR protocol. Can anyone tell me why my plot looks like this? I'm using Taqman advanced microRNA assay and universal PCR mastermix no amperase...
15 August 2020 5,692 3 View
On the basis of in-silico analysis we hypothesized that a long non-coding (lncRNAs) interacts with miRNA thus inhibiting that miRNA function in bread wheat. Now I want to isolate this lncRNA-miRNA...
13 August 2020 7,709 2 View
I have tested mirDB and target scan, but they seem to be predicted.
12 August 2020 6,476 3 View
I want to understand what kind of variations occur on long ncRNA. Like for example missense mutations, point mutations, frameshift mutations etc. Similarly, for pseudogenes what are the variant...
10 August 2020 4,491 1 View
I wanna do miRNA profiling in serum and plasma using SYBR green and I wanna know the best and cheap kit to extract miRNA. Which way is better: Extract miRNA directly using available kits followed...
06 August 2020 5,332 6 View
I have been running a small test experiment, extracting RNA from FFPE DLBCL blocks, and analysing miRs in the samples. So far I have been using the TaqMan™ MicroRNA Assay, which require a...
05 August 2020 1,340 1 View
Hello, As the question shows, I'm looking for methods, preferably inexpensive and nontoxic, to remove leaf trichomes without damaging the leaf stomata. I am working with leaves from the woody...
02 August 2020 1,024 4 View
Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...
31 July 2020 9,860 3 View
Dear all, I´m experienced in analyzing datasets of gene expression, but for my new project I don´t seem to find a dataset with different time points that is fundamental for my objective. I did...
30 July 2020 7,455 2 View
We know very well that RNA silencing is a potential ways by which small plants such as tomato, tobacco etc protect themselves against viruses. I was just curious to know if RNA silencing is also...
21 July 2020 363 2 View
I am working on a project to look for valuable miRNAs for cancer diagnosis. I need to get the miRNA from exosomes. Has anyone ever done this before and how did you do that? Thank you!
15 July 2020 9,434 6 View