Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
I am looking for any user friendly online tools for predicting the secondary structure for 3'UTR.
08 August 2019 8,015 8 View
I am looking to investigate miRNAs to compare expression between patients and healthy controls. The patients are likely to have abnormal lymphocyte counts. Can anyone advise on the preferred...
08 August 2019 4,725 3 View
Hello everybody, I am currently extracting total RNA including microRNA from serum using miRNeasy Serum/ Plasma kit Catalog Number 217184 from Qiagen. Within the procedure, we add a Spike -In...
08 August 2019 5,566 3 View
Hello, I'm wondering how to get a list of microRNA or lncRNA or other types of RNA that is associated with a certain type of cancer such as breast cancer? I see in research paper they often put...
02 August 2019 4,256 4 View
I have downloaded the miRNA-seq data for pancreatic cancer from TCGA. Now, I would like to see the expression of a particular miRNA across all the samples - or at least to classify samples into...
10 July 2019 5,038 1 View
I am working with microRNAs from human plasma samples. I have normalized the initial input concentration of the extracted microRNAs for the reverse-transcription step using Qubit. I would like to...
07 July 2019 5,615 5 View
I have motifs that were obtained methylated genes. I want to check whether there is a miRNA targeting the motifs. Do you know that there is a tool for this?
07 July 2019 4,548 2 View
I am looking for a tool or methods which can help me to choose the stretch of sequence from my target miRNAs which can be used to design siRNA in order to inhibits its expression in cultured cells.
07 July 2019 8,400 3 View
Characteristics U6 snRNA that makes them useful as controls.
07 July 2019 7,053 4 View
I need to analyze pre-miRNA expression using TaqMan predesigned gene expression assays. The company confirmed that all the steps of retro-transcription and qPCR are the same as for long RNAs...
07 July 2019 8,049 4 View
We are interested in AML cell transfections (respectively MOLM14 and MV4-11) by miRNAs or siRNAs. We tried several electroporation or amaxa protocols , but transfection efficiencies were very low...
07 July 2019 8,933 3 View
I'm planning on extracting microRNA's from aged drosophila heads for sequencing but due to the amount of heads I'll need to cover all my experimental groups I may have to raise and collect heads...
07 July 2019 277 4 View
I need to perform miRNA analisys on DAPI stained cells. Have you done something similar? DO you know if DAPI staining will permeabilize cells and cause miRNA loss?
07 July 2019 6,885 6 View
I am doing miRNA RT-qPCR and gel electrophoresis to confirm the nature of the amplicons. The bands in agarose gels do appear in the desired position without non-specific bands, but my concern is...
07 July 2019 210 9 View
I am studying computational methods related to microRNAs to identify their diagnostic and therapeutic ability at the presence of prostate cancer. I have planned to analyse the TCGA PRAD data and...
07 July 2019 7,218 2 View
07 July 2019 1,786 1 View
Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.
18 June 2019 1,202 3 View
Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...
15 June 2019 5,422 6 View
Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...
14 June 2019 2,305 9 View
I am asking for help in identifying known miRNAs in plants. After bioinformatic analyzes, I have a list of miRNAs identified and I do not know what kind of criteria adopt in order to find among...
13 June 2019 3,572 10 View
I have used mircury exosome serum/plasma kit along with trizol for extraction of my miRNAs from human plasma. RNA concentration was 623.8ng on nanodrop with 260/280 ratio of 2.12. For RT-PCR and...
12 June 2019 3,776 4 View
I forgot to put my taqman miRNA assay(thermofisher) at -20degree. It was at 4degree for 48hours. Will it be working?
12 June 2019 6,657 5 View
06 June 2019 3,606 5 View
Hi! I am trying to isolate RNA from human plasma, specifically looking at circulating miRNA. I am uncertain about the best methods for storage of the samples. I have heard different things about...
06 June 2019 9,042 3 View