Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
Hi, Our wet-lab results inhibit bacterial growth against certain compounds. We are interested to predict its possible targets from bacteria. I came to know about Inverse docking but I did not find...
20 April 2022 6,622 2 View
Hello i want to do some analysis in Plink like Run Of Homozygosis and look for some scrpits to start, thanks for help i have a data base in vcf format
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I am coating the well with blank 1X PBS and then Block it with complete RPMI media and after blocking give these well PBS wash and seed the stimulated PBMCs which have Antigen Secreting cells.
27 February 2022 1,048 3 View
I want to know about different in-silico methods or tools through which I can predict the target protein of any cell for the designed drug or peptide.
21 February 2022 7,157 1 View
Hello, I intend to analyze my DGGE gels and need a good software to do the same. Specifically, I want to check the band densities and construct dendograms. Can anyone suggest any good software...
09 February 2022 10,051 3 View
Something about our protocol or reagents is wrong because we couldn't manage to succeed.
09 February 2022 7,739 0 View
Hello I am working on viral infection in PK-15 cells and then IFA in 96 well plates. After fixing the cells with 50%methanol and acetone for overnight at 40C and IFA, the wells turn completely...
22 December 2021 8,485 2 View
Dear All, i came across different papers (mostly published between 2000 and 2010, i.e. "Predicting Arterial Stiffness From the Digital Volume Pulse Waveform") in which formulas and associations...
09 November 2021 6,902 3 View
Regarding genetic testing
29 October 2021 6,571 1 View
Dear all, I am working on differential expression of 5 miRNAs in 3 different accessions of tomatoes named Solanum pimpinellifolium, Solanum lycopersicum, and Solanum habrochaites under abiotic...
23 October 2021 6,835 3 View
I am running and experiment where I use cryopreserved PBMCs as the following: 1) thawing and incubation on RPMI-1640 in CO2 incubator overnight 2) activation with IFN-alpha for 15 min 3) fixation...
15 October 2021 7,013 3 View
We have a problem with MIN6. I don't know certainly the number of passage that they are. The first passage that we have has an appropriate reaction on adding glucose, but the fourth does not...
13 October 2021 9,073 0 View
Hello. I am working on differential expression of 5 miRNAs in 3 different accessions of tomatoes named Solanum pimpinellifolium, Solanum lycopersicum, and Solanum habrochaites under abiotic...
10 October 2021 1,199 2 View
I am performing magnify chromatin immunoprecipitation on thalamic tissue samples to determine if the primers we have for VGAT work via q-PCR. Before I ask my question, I want to put out there...
26 September 2021 7,444 0 View
Hi, I am interested in a chromatin state over one of the promoters in different cell lines. I would like to check it by ChIP-qPCR. The question is how to show it? Is a one set of primers...
26 August 2021 2,623 1 View
Hello all, I am fairly new to the world of RNA and sncRNA, so I appreciate any input. I am currently trying to amplify miRNA sequences with primers that I generated using the miRprimer algorithm...
16 August 2021 1,356 2 View
I am performing miRNA loss-of-function study in cells and using a reporter assay to validate miRNA inhibition. So, I am planning on cloning the entire 3’-UTR sequence of our miRNA target...
09 August 2021 7,287 3 View
Dear ResearchGate community, I am analyzing data from SSR molecular markers (codominant), in plants of Rubus spp. I need to calculate the polymorphic information content (PIC) of my data. Could...
07 August 2021 9,514 0 View
I am planning to perform EMSA for protein-DNA interactions. In order to do so, I need something that blocks the non-specific DNA-protein interactions. People have used Poly(dI.dC) in their assays....
22 July 2021 4,626 0 View
I am looking for host genes coding these lncRNAs and mRNAs.
18 July 2021 6,389 2 View
Human RNA extraction from serum: Can someone please help me with the protocol?
07 July 2021 5,638 6 View
hello everyone, I have a results for interaction between miRNA – lncRNAs and interaction between mRNA- lncRNA. I want to draw network in cytosape but faced error such as (Gene identifier Error,...
23 June 2021 7,980 0 View
Does anyone have suggestions for linking a list of lncRNAs to their target mRNAs? I am looking to generate networks consisting of lncRNA-mRNAs that are activated or downregulated in response to a...
21 June 2021 2,266 1 View
Dear all, I found many different result for response time of ionic gated transistors, from second to microsecond, and I think we do not have a unique definition or method for measuring response...
16 June 2021 3,127 0 View