Gene regulation with miRNA: mechanisms, implications, techniques | Contact experts in microRNA to get answers
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Questions related to microRNA
Hi, I am looking for an anti-P-src antibody which interact with human CD44. Tyr 418 was used in the literature but only Tyr 416 on Cellsignaling website. Are they the same?
17 February 2020 6,304 4 View
Has anyone tried it before? if yes kindly provide a protocol for the same. Thanks in advance.
08 February 2020 6,391 2 View
We are studying on miRNA RT-QPCR. How can we detect mature miRNA quality without bioanalyzer? İs there any cheaper way to assess miRNA quality for miRNA enrichment protocol (this protocol enable to
02 February 2020 7,551 3 View
I am not sure for what time miRNAs remain stable in and what are the storage conditions and best method for high yeild
02 February 2020 3,757 1 View
Anyone has been able to purify miRNAs from EBCs (exhalate breath condensate)?
02 February 2020 2,668 0 View
Hi, I need help from someone who has worked with exosome RNA (miRNAs) isolation. I'm working with MDA231 secreted exosomes but I have failed in isolate RNA from these vesicles. Usually I obtained...
02 February 2020 6,769 2 View
This is based on RNA-Seq data, where I have compared WT and KO samples from a microRNA knockout. Many thanks!
02 February 2020 5,745 2 View
Dear Researcher I'm going to work on miRNAs in Cystic Echinococcus (germinal layer and protoscolex). What are the most important target genes for miR-71,miR-9,miR-10 and Let-7 in this...
02 February 2020 8,916 1 View
Potential of miRNA and svRNA-N antagomirs as antivirals Missed in Chinese (ethnicity) This is engineered virus, Will stop after 35 days. Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1,...
26 January 2020 9,509 2 View
I am a starting PhD student with basic programming skills in python and rather good data-analytical skills in R. I plan to perform RNA sequencing analysis in the near future. To get a better grasp...
20 January 2020 322 3 View
I am looking for a database or a software that helps me to draw miRNA network (miRNA and their Targets). Thank you.
18 January 2020 479 4 View
Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...
18 January 2020 7,558 3 View
I have a list of six miRNAs and I am searching for a tool that help me to test the interaction between them. Any suggestions please! Thanks.
10 January 2020 7,573 3 View
In Beta-glucosidase assay, after translating the amount of p-nitrophenol release from the standard curve, How to calculate the enzyme activity?
03 January 2020 443 3 View
Dear Researchers, 1- I am working on RNA seq, and now I have transcriptome data and I have the different genes of interest from different resources, but I am looking for their full-length CDs...
02 January 2020 9,491 4 View
Hi, I need to have a dataset including the expression level of a particular miRNA and a particular mRNA in tumor (digestive cancer :Colorectal+gastric)+ its matched normal tissu across samples...
01 January 2020 3,031 3 View
I am working with circulatory miRNAs derived from plasma. My study involves 4 case groups and 1 control group. I have tested the utility of 3 miRNAs as endogenous controls and none of them have...
01 January 2020 8,970 4 View
I want to encapsulate miRNAs into liposomes, and it doesn't seem to work with my mini-extruder, so I am ready to use the service of a competent company. Any suggestions?
01 January 2020 8,860 2 View
01 January 2020 2,846 3 View
can someone please help me with choosing the best way to search for miRNA and ncRNA changes in bone marrow transplant patients before and after surgery? which system do I look for changes? which...
01 January 2020 6,851 8 View
Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.
01 January 2020 7,252 2 View
Hello Dears, I am going to analyse selected miRNA in schizophrenia patients samples (plasma, serum, PMNCs). is the good and stable internal control can be used. pilot studies showed that U6 is not...
01 January 2020 8,456 2 View
01 January 2020 2,661 4 View
01 January 2020 9,153 4 View