A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
I need a solid form of hydrogen peroxide, one that will give a high concentration of hydrogen peroxide when dissolved. Urea peroxide is one example, as is sodium percarbonate. Can you suggest others?
12 December 2017 5,825 4 View
Protein Misfolding leads to amyloid fibril formation through the pathway: Monomer to Oligomer to Protofilament and then Matured Fibril. While investing the cytotoxicity of amyloid fibrils, it has...
12 December 2017 9,264 2 View
I have a limited volume of a polyclonal antibody and I look to use it as efficiently as possible. I have aliquoted the antibody and stored the aliquots at -80°C. Whenever I need to perform an...
29 October 2017 9,737 7 View
How can one quantify capture efficiency of antigens in a microfluidic immunoassay chip ? For example, if we have a anti-IgG coated microfluidic chip and we want to know the capture efficiency of...
18 October 2017 1,742 6 View
How can a protein from any organism (parasite, virus, bacteria...) be identified as a potential antigen and for the detection of that organism presence in another one, via antibody detection, with...
10 October 2017 9,141 4 View
Dear all. We do a lot of cytokine and antibody measurements in our lab using ELISA and Luminex etc. There are often notable differences between serum, EDTA- and heparin plasma, as is well-known...
20 September 2017 2,376 2 View
I´m working in the development of a FPIA to detect antibodies against a viral protein of 24kDa. Acording to the sequence, my protein has 9 lysines and 1 NH2 terminal group, so I calculate 10...
09 September 2017 9,095 7 View
Are the antibodies or the solutions? Or something else
09 September 2017 1,472 0 View
I am working on capturing E.coli in immunoassy with streptavidin nanoparticle. Capture antibody linked beads and detection antibody linked nanoparticle. Through gas generation to read out, it...
06 June 2017 5,042 9 View
Hi there, I'm developing a saliva steroid immunoassay based test. With my reaction conditions (which dont have anything esoteric in it), we see better performance with saliva that has been stored...
04 April 2017 2,452 4 View
04 April 2017 6,120 3 View
I;m working with THP-1 cell line. my problem is that from last ffew weaks cells are getting adhered to well plate after PMA stimulation (50 ng/ml) but didn;t get spread ir they are not showing any...
03 March 2017 7,961 4 View
We want to place a large order, but we are not sure how reliable they are. Our local suppliers costs us a lot more, since they also source it from similar foreign merchants, and we wanted to cut...
24 January 2017 9,236 2 View
I believe they should only have an effect on the diffusion distance and in controlling flow velocity or keeping Reynolds no in the Stokes flow regime. Is there any other significance to this...
01 January 2017 8,124 3 View
I want to measure the uptake of Flagellin after treatment to the cells. Please could anyone having experience for this experiment give me some suggestion. I tried to detect by Intracellular...
12 December 2016 1,825 1 View
For Immunolocalization
02 September 2016 4,619 1 View
We are going to make a LFIA strip with gold nanoparticle conjugation to detect liver enzyme. I need to make a business case and fixed a selling price. If anybody share their idea that would be...
10 August 2016 7,882 3 View
Can anyone recommend a reliable for-hire service for performing assays of multiple immune/endocrine/metabolic biomarkers in rat serum? I have found some companies that probe for a few common...
08 August 2016 4,412 0 View
08 August 2016 9,806 4 View
There are number of methods for surface modification/functionalization with PEI, using N-hydroxysuccinimide (NHS), NHS derivatives, EDC, EDC derivatives etc. but no comparative analysis or...
08 August 2016 8,158 2 View
Either by immunoassay or enzymatic. And where can I purchase this assay from, preferably in the UK. Thanks
07 July 2016 1,686 3 View
We've tried a few protocols on our autostainer, but haven't had got anything working yet. I have heard rumors of people getting it to stain rat tissue . I specifically want to stain vascular...
07 July 2016 8,922 3 View
I need the determination of nitrotyrosine concentrations in arotic homogened by ELISA but I have not protein standard .What will I do? and I can comparison the nitrotyrosine concentrations with...
07 July 2016 7,301 1 View
Dear all, I am doing a lateral flow immunoassay. I know many people are suffering non-specific binding on test line, but I am suffering from no color reveals. I first conjugate my MMP9...
06 June 2016 1,028 8 View