Dear all,
I am doing a lateral flow immunoassay.
I know many people are suffering non-specific binding on test line, but I am suffering from no color reveals.
As a result, there should have a color but didn't. I am sure the MMP9 can be used in sandwich assay because they are tested by abcam. But in this high concentration analyte still can't be seen. However, control line is okay made by anti-IgG antibody, means that conjugate procedure is okay,
Do anyone have some recommendation??
I still can't figure out the reason.
If any of you need some detail information, i would reply as soon as possible.
Best regard,