A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
I´m performing DTH assays in mice. I usually immunize with 100 ug OVA in complete Freund´s adjuvant (1:1 mixture) by a subcutaneous injection, wait for two weeks and then inject 100 ug...
09 September 2013 3,306 5 View
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09 September 2013 1,968 0 View
I want to use an antibody as proteasome marker in IFF assays. This antibody can not be of a mouse or a rabbit.
09 September 2013 8,752 0 View
04 September 2013 1,545 4 View
We are dealing with an unknown/yet to be identified virus suspected to belong to chicken coronavirus [infectious bronchitis virus]. There was a history of high mortality and evidence of kidney...
12 August 2013 7,975 12 View
I isolated cytoplasm and nucleus fractions from human cancer cells. These two fractions were probed with p-STAT3 by typical Western blots using CS primary Mabs. We used alpha-tubulin and laminin...
08 August 2013 9,023 4 View
When I perform immunoassay using a commercial antigen(purchased from abcam) it works but when I use a positive serum sample it does not work. Can any one let me know how to remove these inhibitors...
08 August 2013 613 3 View
I want to check the level of active IL-1beta in media from cultured human macrophages. My problem is I do not get the cleaved form of IL-1beta. I always get the 37 kDa. Usually I use the acetone...
08 August 2013 2,576 26 View
Cytokines of mesenchymal cells
08 August 2013 6,462 6 View
08 August 2013 2,745 13 View
I am trying to raise the polyclonal antibodies against my peptides in mice or rabbit but its size is very small, only 5.7 K Da. But before going through it I want to ask if anyone has experience...
08 August 2013 7,631 4 View
plasma extraction using ETOH
07 July 2013 1,924 0 View
In our experiment, monocyte-derived THP-1 macrophages was used for infection with pathogenic bacteria and after Transmission electron Microscope Image, the main problem is, how do we detach theses...
07 July 2013 3,669 13 View
Flow cytometric analysis for this cancer is usually performed with monoclonal antibodies, which are expensive and difficult to access for a huge part of the population. Any alternative detection...
07 July 2013 5,602 1 View
5ppb of phenobarbital have been spiked into blank blood. This have been replicated by 10 times. However, ELISA results show that all 10 of the replicates contains LESS than 5ppb (average of...
07 July 2013 1,543 1 View
DC 3.2 cell line dissociation for mhcII detection?
07 July 2013 2,840 1 View
I'm planning to do some M1 vs M2 macrophage work and am working out the best way to distinguish these subsets. I know you can try this via flow cytometry, however I'm yet to find a suitable Arg-1...
07 July 2013 4,069 16 View
I did a little test to see if my substrate and conjugated Ig were ok. When I added 1ul of anti Ig-Y (secondary conjugated antibody) directly to 1ml of my TMB working solution it turned orange...
07 July 2013 3,480 5 View
I want to measure Hsp60 in plasma samples but I am using monoclonal capture antibody with ascites fluid and diluted it in PBS but the assay did not work , and the optical density values were very...
07 July 2013 8,499 3 View
I am looking for user experiences with reliability and post-sale service with these company products.
07 July 2013 1,742 0 View
I'm trying to use T regulatory cell for the treatment of Sjogren's Syndrome. I have found several papers using Treg for the treatment of other diseases, but the protocols aren't detailed.
07 July 2013 2,600 3 View
03 July 2013 4,425 1 View
02 July 2013 8,769 3 View
I have been working to develop a microfludic protein analysis system and the current prototype system utilizes eppendorf-type vials to contain samples and protein reagents. The vials are...
17 June 2013 9,948 6 View