A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
I would like to develop LFIA where I am trying to use mobile phone camera or any android software as a detector.
06 June 2016 303 0 View
Hi, I am planning to do some luminescent assay to measure ATP production of my samples. I have some black 96 well plates with clear bottom. I was wondering if I could use them for the assay? Also,...
06 June 2016 1,691 4 View
Dear Colleagues. I need to co-culture two similar cell lines (one of them is manipulated and another one is wild type); Additionally, manipulated cell line with HUVECs cell line.I want to buy...
06 June 2016 3,785 4 View
I am trying to purify FITC primer (small DNA of 24 bp) the first step was dissulting through a column which contain at the last step washing with Buffer that contain methanol 50%...
11 May 2016 2,347 1 View
05 May 2016 4,581 1 View
I'd like to know if it would be better to increase the number of cells plated or increase the peptide concentration? Which one would result in a better increase in spot formation.
04 April 2016 10,265 5 View
I have mouse brain (cortex and hippocampus) which has been been put in mPER and stored at -20. I would like to extract protein to run an immunoassay. Would anyone have a protocol for this. Thanks!
04 April 2016 3,064 2 View
I recently did a protein extraction from differenciated 3T3-L1 cells . The fat form a layer at the top and cell debris form a pellet. At this point,I tried to remove the fat by pipetting or go...
04 April 2016 4,838 6 View
A way to adhere cells in glass covers.
04 April 2016 8,024 6 View
04 April 2016 9,926 5 View
How to immunofluorescence staining of the Kinetoehore on the microtubules of the oocyte
22 March 2016 4,358 1 View
How to avoid precipitation
15 March 2016 9,172 2 View
Rhodopsin in Drosophila is 35kDa membrane protein. Do you know some good homogenization buffer? In the literature I see that most of the people use Laemmli sample buffer and just simply smash the...
10 March 2016 7,481 8 View
The challenging part of this is that once a tumor has collapsed, presumably infiltrating neutrophils will "mop up" and look like granulocytic MDSC's i.e. ly6c mid, ly6g hi, cd11b+.
08 March 2016 3,278 3 View
ChIP or IP
03 March 2016 2,101 1 View
03 March 2016 7,151 4 View
03 March 2016 2,319 1 View
It goes to an extent that while one trial gives good results (positive control coming down with bait while negative control does not), another trial shows me that none of my prey proteins are in...
03 March 2016 8,490 13 View
I need identify foreign body giant cells using immunochemistry or flow cytometry. Is it possible?
03 March 2016 773 0 View
03 March 2016 4,369 2 View
I learned PMA-ionomycin can use this procedure but i don't know whole process. How many days do i incubate jurkat cells and what are other chemichals that i need? Thanks
29 February 2016 5,051 1 View
02 February 2016 2,258 1 View
I am trying to achieve multiplexing in rapid tests..for this to achieve which one will serve as better platform lateral flow or flow through membrane assay..??
02 February 2016 3,412 0 View
I need a to perform an immunoassay of TNF-alpha on human serum. I am looking for advise on brands that can offer a good (the best if possible) balance price/quality/simplicity. If you could share...
12 December 2015 6,848 3 View