A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
2,297 views 358 posts
Questions related to Immunoassay
Dear All, I need to design a sandwich lateral flow immuno-assay to detect two concentration ranges of the same antigen i.e. one test line can detect lower concentration of antigen (
27 September 2018 8,956 1 View
Any ideas about defect detection method/approach as to pre-QC the nitrocellulose membrane used for lateral flow immunoassay, non-destructive way? Many other industries use a hand held device to...
09 September 2018 3,041 4 View
I'm developing specific IgE detection kits for food, inhalant and others with immunoassay system(line blot). It is difficut to choose membrane, allergens, the components of sample diluent,...
09 September 2018 9,759 0 View
I will perform an immunoassay to detect the production of iNOS in macrophages by flow cytometry. I need to know what concentration of surfactant I should use to permeate the plasma membrane. Does...
19 August 2018 4,950 3 View
08 August 2018 5,613 4 View
i obtained absorption values after immunoassay of irisin now i want to make graph for finding of irisin concentration. Kindly elaborate method for this
01 August 2018 7,779 2 View
My School and Department are in the process of purchasing a Luminex 200 system for detecting and quantifying targets from plasma/serum and cell culture. I would very much appreciate any...
23 July 2018 8,931 4 View
why immunoassay and immunosensor are different?
06 June 2018 5,154 0 View
I'm interested in modifying a polyacrylamide gel-based immunoassay to use aptamers in place of antibodies. In this assay, the proteins are covalently immobilized within the polyacrylamide gel via...
16 May 2018 5,630 2 View
05 May 2018 3,655 2 View
Hi i'm performing dot blot immunoassay, using nylon membrane Becaus my antibodies are anti a short peptide and not the whole native protein(and not from terminal areas) I'm afraid the native...
05 May 2018 785 5 View
I am doing some immunoassays and cell-based assays against a common human pathogen (high prevalence, routine vaccination in many countries). Most commercial sources of "normal" human serum and IgG...
18 April 2018 8,680 1 View
The first method is silanization of PDMS surface with 50% v/v in ethanol followed by treatment with Glutaraldehyde which presents a site for antibody binding. In the second method BSA is passively...
05 April 2018 526 4 View
04 April 2018 3,627 1 View
After log-in I don's find Roggenbuck's paper at Antiphospholipid antibodies detected by line immunoassay dif... What's to do? Best regards Martin
04 April 2018 5,069 2 View
We are making microchannels by machining in Plexiglas (PMMA). We want to cover the same with Pressure sensitive adhesive tape (PSA tape) to close the channels to improve turnaround time of our...
04 April 2018 9,784 1 View
04 April 2018 6,118 5 View
In the indirect ELISA being performed, when should the stop solution (H2SO4) be added after the addition of substrate (TMB)...? It has been observed that adding H2SO4 after 2 minutes and 5...
05 March 2018 9,425 6 View
..................
02 February 2018 2,597 1 View
What would you recommend? It should be cheap and of constant / defined composition, comparable to defatted milk powder (NFDM). Thanks!
11 January 2018 7,708 6 View
Hello everyone! I am doing half strip test for Lateral low immunoassay. Here is my detail information about the experiment: 1. Size of Gold nanoparticles that I used is 15 nm. 2. GNPs solution...
01 January 2018 8,658 6 View
We have developed a fiber optic sensor and we wish to evaluate them for biosensing. Is there any standard antigen-antibody pair for immunoassay, namely direct and sandwich formats. For example...
19 December 2017 6,737 3 View
I wish to exchange citrate on my 40nm gold particles for a thiol (prochimia.com/products/product/104), however, I always seem to aggregate the gold particles: the initial red colour goes blue, or...
12 December 2017 1,260 5 View
12 December 2017 6,262 4 View