Solve standard curve equation?

I have a standard curve for an ELISA, and want to find unknown concentrations from absorbances. my polynomial equation is: y= - 1e-06x^2 + 0.0032x + 0.2562 How can I solve this to find analyte...

07 August 2019 3,433 3 View

What is the best method for finding unknown concentrations from optical density?

We have an in house ELISA, how do you calculate unknown concentrations from optical density values? We produced a standard curve but don't know whether to use sigma plot or excel. Also, Which...

07 August 2019 9,881 6 View

By how much is CVD risk reduced for a reduction in TC:HDL ratio?

Does anyone have papers showing statistics of CVD risk reduction with improvements in TC:HDL ratio?

10 November 2018 5,083 1 View

Reduction in fasting Insulin levels and associated CVD/T2D risk?

Does anyone have papers relating fasting insulin levels and associated CVD risks?

10 November 2018 1,898 4 View

What is a clinically relevant reduction in 24-hr Blood Pressure?

Is there a threshold by which 24-hr Blood Pressure needs to be reduced by to be clinically relevant? or is it possible to use the clinic-based blood pressure thresholds? any papers would be...

10 November 2018 9,731 3 View

Statistical difference between correlations?

Which test should be run for determining statistical differences between 2 (r values) correlations (correlated correlations)? I am looking to see if there are any differences between a placebo...

09 October 2018 6,566 5 View

What is the average dropout rate of human RCTs involving dietary supplements?

Any papers would be appreciated.

09 October 2018 2,335 2 View

What are normal serum concentrations of ACE enzyme in humans?

Does anyone have papers showing angiotensin-converting enzyme concentrations in serum in humans, preferably Metabolic Syndrome humans? Thanks

08 September 2018 6,238 3 View

Bland Altman for repeated measures reliability?

Can you use Bland-Altman for more than 2 repeated measures in the same person? I have measured the same dependent variable 5 times in the same person.

06 July 2018 9,472 3 View

Should Partial Eta Squared be used as an effect size?

When conducting a repeated-measures ANOVA between 2 conditions, SPSS outputs report Partial Eta Squared for effect size. Should this be used or is there a better alternative for effect size?

05 June 2018 6,676 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View